Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye

Saikiran, Maryala; Sato, Daisuke; Pandey, Shyam S.; Hayase, Shuzi; Kato, Tamaki

doi:10.1016/j.bmcl.2017.07.057

Cited by 10 publications

(9 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conjugation to a targeting ligand allows for multiple signaling strategies. Dyes can be attached to enzyme labile quenching groups which are cleaved under particular cellular conditions to restore signal . Similarly, multiple dyes can be attached to a single targeting ligand to utilize dye aggregation as a means of signal generation .…”

Section: Application Of Squaraine Dyesmentioning

confidence: 99%

“…Saikiran et al developed a turn-on sensor for human neutrophil elastase (HNE) detection using a dimerized squaraine dye linked by a B-Ala-Ala-Pro-Ala-Lys-(Obzl) peptide . Specifically, elastase recognizes and hydrolyzes the Ala-Pro-Ala sequence of the peptide.…”

Section: Application Of Squaraine Dyesmentioning

confidence: 99%

“…Fluorescence spectral recovery after incubation with elastase (bottom left) and change in fluorescence intensity over time during incubation with different concentrations of elastase (bottom right). Figure adapted from ref with permission. Copyright 2017 Elsevier.…”

Section: Application Of Squaraine Dyesmentioning

confidence: 99%

“…Dyes can be attached to enzyme labile quenching groups which are cleaved under particular cellular conditions to restore signal. 91 Similarly, multiple dyes can be attached to a single targeting ligand to utilize dye aggregation as a means of signal generation. 92 Encapsulation within nanostructures presents fewer signaling options and largely relies on the enhanced permeability and retention effect to deliver dyes to tumors.…”

Section: Synthesis Of Squaraine Dyesmentioning

confidence: 99%

See 3 more Smart Citations

Squaraine Dyes: Molecular Design for Different Applications and Remaining Challenges

et al. 2019

View full text Add to dashboard Cite

Squaraine dyes are a class of organic dyes with strong and narrow absorption bands in the near-infrared. Despite high molar absorptivities and fluorescence quantum yields, these dyes have been less explored than other dye scaffolds due to their susceptibility to nucleophilic attack. Recent strategies in probe design including encapsulation, conjugation to biomolecules, and new synthetic modifications have seen squaraine dyes emerging into the forefront of biomedical imaging and other applications. Herein, we provide a concise overview of (1) the synthesis of symmetrical and unsymmetrical squaraine dyes, (2) the relationship between structure and photophysical properties of squaraine dyes, and (3) current applications of squaraine dyes in the literature. Given the recent successes at overcoming the limitations of squaraine dyes, they show high potential in biological imaging, in photodynamic and photothermal therapies, and as molecular sensors.

show abstract

Section: Application Of Squaraine Dyesmentioning

confidence: 99%

Section: Application Of Squaraine Dyesmentioning

confidence: 99%

Section: Application Of Squaraine Dyesmentioning

confidence: 99%

Section: Synthesis Of Squaraine Dyesmentioning

confidence: 99%

See 2 more Smart Citations

Squaraine Dyes: Molecular Design for Different Applications and Remaining Challenges

et al. 2019

View full text Add to dashboard Cite

show abstract

“…As such, developing highly sensitive and specific activity-based fluorescent probes for HNE detection is of great interest. In the last two decades, by mimicking the native protein substrate elastin, some chemical probes, either peptide-based probes − or small-molecule based synthetic probes, − were reported as fluorogenic substrates to measure HNE activity and demonstrated applicability for drug discovery and disease diagnosis. However, suffering from the limitation in the limit of detection (LOD), tissue penetration, or biocompatibility, few of them could be applied to HNE detection in vivo , and none of them has been translated into clinical diagnosis.…”

mentioning

confidence: 99%

Human Neutrophil Elastase Activated Fluorescent Probe for Pulmonary Diseases Based on Fluorescence Resonance Energy Transfer Using CdSe/ZnS Quantum Dots

et al. 2020

View full text Add to dashboard Cite

There is an increasing demand for effective noninvasive diagnosis against common pulmonary diseases, which are rising sharply due to the serious air pollution. Human neutrophil elastase (HNE), a typical protease highly involved in pulmonary inflammatory diseases and lung cancer, is a potential predictor for disease progression. Currently, few of the HNE-targeting probes are applicable in vivo due to the limitation in sensitivity and biocompatibility. Herein, we reported the achievement of in vitro detection and in vivo imaging of HNE by incorporating the HNE-specific peptide substrate, quantum dots (QDs), and organic dyes into the fluorescence resonance energy transfer (FRET) system. The refined nanoprobe, termed QDP, could specifically measure the HNE with excellent sensitivity of 7.15 pM in aqueous solution and successfully image the endogenous and exogenous HNE in living cells. In addition, this nanoprobe enabled HNE imaging in mouse models of lung cancer and acute lung injury, and the HNE activity at high temporal and spatial resolution was continuously monitored. Most importantly, QDP successfully discriminated the serums of patients with lung diseases from those of the healthy controls based on the HNE activity determination. Overall, this study demonstrates the advantages of a FRET-system-based nanoprobe in imaging performance and provides an applicable tool for in vivo HNE detection and pulmonary disease diagnosis.

show abstract

Dual emissive bispyrene peptide probes for highly sensitive measurements of trypsin activity and evaluation of trypsin inhibitors

Sato

Kondo

Kato

2018

Bioorganic & Medicinal Chemistry

View full text Add to dashboard Cite

Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye

Cited by 10 publications

References 32 publications

Squaraine Dyes: Molecular Design for Different Applications and Remaining Challenges

Squaraine Dyes: Molecular Design for Different Applications and Remaining Challenges

Human Neutrophil Elastase Activated Fluorescent Probe for Pulmonary Diseases Based on Fluorescence Resonance Energy Transfer Using CdSe/ZnS Quantum Dots

Dual emissive bispyrene peptide probes for highly sensitive measurements of trypsin activity and evaluation of trypsin inhibitors

Contact Info

Product

Resources

About