Efficient Microspore Embryogenesis and Haploid Induction in Mutant Indica Rice (Oryza sativa L.) Cultivars

Tajedini, Samira; Fakheri, Barat Ali; Niazian, Mohsen; Mahdinezhad, Nafiseh; Ghanim, Abdelbagi Mukhtar Ali; Pour, Ali Kazemi; Ingelbrecht, Ivan; Shariatpanahi, Mehran E.

doi:10.1007/s00344-022-10709-y

Cited by 4 publications

(3 citation statements)

References 59 publications

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“…In the plant regeneration experiments, the genotypes, combination of growth regulators and quality of calli (see above) significantly influenced the plant regeneration efficiency. Zeatin was applied as an exogenous growth regulator in combination with other hormones in rice AC experiments [26,28,35], while added zeatin had an adverse effect on both callus induction and plant regeneration in AC of indica rice genotypes. The different combinations of NAA, BAP and Kinetin were applied for plant regeneration in rice AC [11,12,30].…”

Section: Effect Of Exogenous Growth Regulators On the Regeneration Of...mentioning

confidence: 99%

“…According to published protocols, different combinations of growth regulators (BAP, NAA, kinetin and zeatin) were successfully applied to produce green plantlets and increase plant regeneration efficiency [5,7,8,[10][11][12][13][14][21][22][23][24]26,27,30,31,34,35]. The efficiency of green plantlets production was improved further by the increased quantity of gelling agent and added proline to the regeneration medium in rice AC [30,36].…”

Section: Introductionmentioning

confidence: 99%

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Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Lantos

Jancsó

Székely

et al. 2023

Plants

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Anther culture is an efficient biotechnological tool in modern plant breeding programs to produce new varieties and parental lines in hybrid seed productions. However, some bottlenecks—low induction rate, genotype dependency, albinism—restrict the widespread utilization of in vitro anther culture in rice breeding, especially in Oryza sativa ssp. indica (indica) genotypes, while an improved efficient protocol can shorten the process of breeding. Three different induction media (N6NDK, N6NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested with five indica rice genotypes to increase the efficiency of in vitro androgenesis (number of calli and regenerated green plantlets). The production of calli was more efficient on the N6NDK medium with an average 88.26 calli/100 anthers and N6NDZ medium with an average of 103.88 calli/100 anthers as compared to Ali-1 with an average of 6.96 calli/100 anthers. The production of green plantlets was greater when calli was produced on N6NDK medium (2.15 green plantlets/100 anthers) compared when produced on to N6NDZ medium (1.18 green plantlets/100 anthers). Highest green plantlets production (4.7 green plantlets/100 anthers) was achieved when mMSNBK1 plant regeneration medium was used on calli produced utilizing N6NDK induction medium. In the best overall treatment (N6NDK induction medium and mMSNBK1 plant regeneration medium), four tested genotypes produced green plantlets. However, the genotype influenced the efficiency, and the green plantlets production ranged from 0.4 green plantlets/100 anthers to 8.4 green plantlets/100 anthers. The ploidy level of 106 acclimatized indica rice plantlets were characterized with flow cytometric analyses to calculate the percentage of spontaneous chromosome doubling. Altogether, 48 haploid-, 55 diploid-, 2 tetraploid- and 1 mixoploid plantlets were identified among the regenerant plantlets, and the spontaneous chromosome doubling percentage was 51.89%. Utilization of DH plants have been integrated as a routine method in the Hungarian rice breeding program. The tetraploid lines can be explored for their potential to offer new scopes for rice research and breeding directions in the future.

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Section: Effect Of Exogenous Growth Regulators On the Regeneration Of...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Lantos

Jancsó

Székely

et al. 2023

Plants

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“…Plant regeneration via androgenesis can be obtained in in vitro culture of anthers or microspores by the application of various inducing factors, among which the stress pre-treatment of explants is considered as the most effective (Tajedini et al 2022). It is believed that reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ) and superoxide radical (O 2 −.…”

Section: Introductionmentioning

confidence: 99%

Gum Arabic influences the activity of antioxidant enzymes during androgenesis in barley anthers

Klajmon

Makowska

Zimny

et al. 2023

Plant Cell Tiss Organ Cult

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This study investigated the antioxidant activity of gum Arabic (GA) during androgenesis in barley anthers. After stress pretreatment the anthers were cultured in the presence of 10 mg L−1 GA (Gm) or on control medium (Cm) and compared with respect to activity of selected antioxidant and respiratory enzymes and endogenous hydrogen peroxide (H2O2) content. The anthers from Cm and Gm differed in the strategy against H2O2 overproduction, in that the total peroxidase (POX) activity and the number of POX isoforms were significantly higher in Gm- than in Cm-cultured material. High POX activity on Gm paralleled with H2O2 decrease, suggesting the utilization of this chemical for the POX-mediated cell wall formation and reconstruction during growth of multicellular structures. The total superoxide dismutase (SOD) activity on Cm and Gm were at similar level for most of the culture period but the activity of MnSOD was dozen times higher on Gm and this coincided with high activity of fumarase and cytochrome c oxidase. It indicates close interplay between efficient antioxidative protection and high metabolic rate accompanying efficient androgenesis. Mass spectrometry analysis confirmed the presence of POX and other antioxidative and defense enzymes in protein fraction of GA used in the experiments, however as revealed by 2,2-diphenyl-1-picrylhydrazyl assay, the Gm and Cm displayed similar total antioxidant capacity. Thus, the effect of GA on androgenic cultures of barley can be linked to its influence on the activity of anther antioxidant system, rather than its native antioxidative properties.

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Haploid System in Mutation Breeding

Amirian,

Shariatpanahi,

Taghiabadi

2024

Sustainable Landscape Planning and Natural Resources Management

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Efficient Microspore Embryogenesis and Haploid Induction in Mutant Indica Rice (Oryza sativa L.) Cultivars

Cited by 4 publications

References 59 publications

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Gum Arabic influences the activity of antioxidant enzymes during androgenesis in barley anthers

Haploid System in Mutation Breeding

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