Efficient method for determination of deoxynivalenol and nivalenol in cereals by gas chromatography

Abstract

We examined analytical method for rapidly determining deoxynivalenol (DON) and nivalenol (NIV) in cereals. Since we used a solvent of high polarity, water, a column of mixed alumina-charcoal-celite, and a column of amberlite for extraction and clean up, we were able to remove partition and dehydration processes. We determined mycotoxin by ECD-gas chromatography after trimethyl-silylation. We made the limits of detemination of DON and NIV at 12 ng/g. When DON and NIV were added to wheat and barley at 120 ng/g level, the recoveries were 101 and 95%, 93 and 92%, and the coefficients of variation were 2.9 and 3.4%, 3.7 and 2.4%, respectively. This method should also be applicable to the analysis of food and feed.