Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Tang, Yuhan; Zhang, Yue; Rosenberg, Julian N.; Sharif, Nadia; Wang, Fei

doi:10.1039/c5ra23519g

Cited by 36 publications

(18 citation statements)

References 44 publications

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“…The extraction of lipids from the microalgal biomass can be performed using polar organic solvents such as methanol, acetone, and ethanol, as well as low‐polarity organic solvents such as hexane, benzene, toluene, diethyl ether, ethyl acetate, and chloroform. Low‐polarity organic solvents alone are unable to extract NLs effectively as they cannot access those that form strong hydrogen bonds with the polar lipids associated with the cell wall . To access these neutral lipids (NLs), a polar organic solvent must be paired with a low‐polarity organic co‐solvent; the polar organic solvent is intended to disrupt the neutral‐polar lipid complexes and the low‐polarity organic solvent aims to solubilize the intracellular NLs.…”

Section: Lipid Extractionmentioning

confidence: 99%

“…To access these neutral lipids (NLs), a polar organic solvent must be paired with a low‐polarity organic co‐solvent; the polar organic solvent is intended to disrupt the neutral‐polar lipid complexes and the low‐polarity organic solvent aims to solubilize the intracellular NLs. Solvent systems containing a mixture of low‐polarity and polar organic solvents generally maximize the extraction efficiency of NLs …”

Section: Lipid Extractionmentioning

confidence: 99%

“…Lowpolarity organic solvents alone are unable to extract NLs effectively as they cannot access those that form strong hydrogen bonds with the polar lipids associated with the cell wall. 43 To access these neutral lipids (NLs), a polar organic solvent must be paired with a low-polarity organic co-solvent; the polar organic solvent is intended to disrupt the neutral-polar lipid complexes and the low-polarity organic solvent aims to solubilize the intracellular NLs. Solvent systems containing a mixture of low-polarity and polar organic solvents generally maximize the extraction efficiency of NLs.…”

Section: Conventional Organic Solvent Extractionmentioning

confidence: 99%

“…Solvent systems containing a mixture of low-polarity and polar organic solvents generally maximize the extraction efficiency of NLs. 43 and co-solvent selection on the distribution of the FAMEs recovered from freeze-dried N. oculata. 61 They showed that scCO 2 with co-solvents such as methanol or hexane could effectively extract the lipids beneficial in biofuel production without the need for extreme temperatures.…”

Section: Conventional Organic Solvent Extractionmentioning

confidence: 99%

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Advances in microalgal lipid extraction for biofuel production: a review

Harris

Viner

Champagne

et al. 2018

Biofuels Bioprod Bioref

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Algal biomass is an attractive feedstock for sustainable biofuel production because of its high growth rate and the fact that it does not compete with food crops. This review examines progress made in the processing and extraction of microalgal lipids as feedstocks for algae‐derived biofuels. The discussion focuses on lipid extraction processes but also mentions drying, cell disruption, and transesterification processes because of their potential effect on the extraction process, and because of the possibility of performing them simultaneously with extraction. Some of the common themes discussed include the benefits of utilizing wet microalgal biomass, combining process steps (process intensification), and the importance of considering the entire life cycle when assessing the ‘greenness’ of a technology. Lipid extraction technologies will need to be improved for microalgal biofuels to compete effectively with fossil fuels, particularly through the development of energy‐efficient extraction methods and the adaptation of these methods for large‐scale production. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Lipid Extractionmentioning

confidence: 99%

Section: Lipid Extractionmentioning

confidence: 99%

Section: Conventional Organic Solvent Extractionmentioning

confidence: 99%

Section: Conventional Organic Solvent Extractionmentioning

confidence: 99%

See 2 more Smart Citations

Advances in microalgal lipid extraction for biofuel production: a review

Harris

Viner

Champagne

et al. 2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

“…5−9 It is possible to maximize the extraction of lipids by decomposing polar lipid complexes with polar organic solvents and solubilizing intracellular lipids with nonpolar organic solvents. 7 However, solvent extraction is usually uneconomic due to consumption of much energy to dry the microalgae as pretreatment and the need to separate bio-oil from solvent after extraction. Tsutsumi et al 10 reported that many environmental hazards and safety problems remain in bio-oil recovery by solvent extraction because a large amount of organic solvent is needed.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Bio-Oil and Nitrogen Recovery from Microalgae Using Two-Stage Hydrothermal Liquefaction with Solid Carbon and HCl Acid Catalysis

2020

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Bio-oil production from microalgae by using hydrothermal liquefaction (HTL) has been conducted extensively in the last decade. In this work, we conducted two-stage HTL of a microalga (Fistulifera solaris, JPCC DA0580) in the presence of 5.0 g/L carbon solid acid or a 0.02−0.50 M HCl catalyst to increase bio-oil yield and nitrogen recovery into the aqueous phase (AP). The first stage (HTL 1), to hydrolyze proteins, carbohydrates, and lipids and elute nitrogen components into the AP, was conducted at 100−250°C for 30−120 min. The second stage (HTL 2), to produce the bio-oil, was conducted at 280−320°C for 0−30 min. The best conditions to obtain a high bio-oil yield and NH 4 + recovery in the AP were 200°C and 30 min of residence time for HTL 1 and 320°C and 0 min residence time for HTL 2. We found that 0.50 M HCl decreased the bio-oil yield while greatly increasing NH 4 + in the AP and decreasing the nitrogen content in the bio-oil. This was probably due to the catalytic effect of HCl promoting hydrolysis of protein and deamination of amino acids during HTL 1. The fractions of water-soluble products were greatly increased by performing HTL 2 in neutral conditions while this maintained low nitrogen content in the bio-oil. From GC−MS analyses of the bio-oil, it was observed that, by using 0.50 M HCl, peak intensities of all the GC peaks decreased and MS spectra of amines decreased. The carbon solid acid had an insignificant influence on bio-oil and NH 4 + yields.

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Development of Cost-Effective High Yielding Cell Disruption Techniques for Microalgae

Chatterjee,

Das,

Bandyopadhyay

2024

Environmental Science and Engineering

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Efficient lipid extraction and quantification of fatty acids from algal biomass using accelerated solvent extraction (ASE)

Abstract: Accelerated solvent extraction optimized for extraction of algal lipids and recovery of polyunsaturated fatty acids.

Cited by 36 publications

References 44 publications

Advances in microalgal lipid extraction for biofuel production: a review

Advances in microalgal lipid extraction for biofuel production: a review

Improvement of Bio-Oil and Nitrogen Recovery from Microalgae Using Two-Stage Hydrothermal Liquefaction with Solid Carbon and HCl Acid Catalysis

Development of Cost-Effective High Yielding Cell Disruption Techniques for Microalgae

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