Efficient isopropanol-butanol (IB) fermentation of rice straw hydrolysate by a newly isolated Clostridium beijerinckii strain C-01

Dalal, Jyotsana; Das, Mriganko; Joy, Sam; Yama, Mohan; Rawat, J. K.

doi:10.1016/j.biombioe.2019.105292

Cited by 12 publications

(10 citation statements)

References 37 publications

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“…Clostridium beijerinckii is a Gram-positive anaerobic solventogenic bacterium that is well known for its ability to produce acetone, butanol, and ethanol (ABE) or isopropanol, butanol, and ethanol (IBE) ( Qureshi and Blaschek 2001 ; Survase et al 2011 ; dos Santos Vieira et al 2020 ) using low-cost carbon sources, such as different straw hydrolysates ( Bellido et al 2014 ; Dalal et al 2019 ), lignocellulosic hydrolysate ( Reddy et al 2020 ), and molasses ( Li et al 2013 ; Fonseca et al 2020 ). In addition, it has been used to produce hydrogen with great success ( Seelert et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Comparative genome analysis of Clostridium beijerinckii strains isolated from pit mud of Chinese strong flavor baijiu ecosystem

Zou

Liu

et al. 2021

G3 Genes|Genomes|Genetics

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Clostridium beijerinckii is a well-known anaerobic solventogenic bacterium which inhabits a wide range of different niches. Previously, we isolated five butyrate-producing C. beijerinckii strains from pit mud (PM) of strong-flavor baijiu (SFB) ecosystems. Genome annotation of the five strains showed that they could assimilate various carbon sources as well as ammonium to produce acetate, butyrate, lactate, hydrogen, and esters but did not produce the undesirable flavours isopropanol and acetone, making them useful for further exploration in SFB production. Our analysis of the genomes of an additional 233 C. beijerinckii strains revealed an open pangenome based on current sampling and will likely change with additional genomes. The core genome, accessory genome, and strain-specific genes comprised 1567, 8851, and 2154 genes, respectively. A total of 298 genes were found only in the five C. beijerinckii strains from PM, among which only 77 genes were assigned to Clusters of Orthologous Genes (COG) categories. In addition, 15 transposase and 12 phage integrase families were found in all five C. beijerinckii strains from PM. Between 18 and 21 genome islands (GIs) were predicted for the five C. beijerinckii genomes. The existence of a large number of MGEs indicated that the genomes of the five C. beijerinckii strains evolved with the loss or insertion of DNA fragments in the PM of SFB ecosystems. This study presents a genomic framework of C. beijerinckii strains from PM that could be used for genetic diversification studies and further exploration of these strains.

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Section: Introductionmentioning

confidence: 99%

Comparative genome analysis of Clostridium beijerinckii strains isolated from pit mud of Chinese strong flavor baijiu ecosystem

Zou

Liu

et al. 2021

G3 Genes|Genomes|Genetics

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“…The isolation method was similar to that described by Weizmann (1935) [13]. Brie y 2% of crushed grains (wheat, gram, barley and millet) were added separately to autoclaved; hot (90-100°C, to counter select against non-spore formers) reinforced clostridial media (RCM) in a 120 mL Wheaton bottle and incubated anaerobically at 35°C for 5 days.…”

Section: Screening and Isolation Of Solvent Producing Bacterial Strainsmentioning

confidence: 99%

“…Batch fermentation was done with the hydrolysate obtained after acid pre-treatment. Sodium acetate and yeast extract was also added to the hydrolysate for fermentation [13]. The hydrolysate was inoculated with 10% (v/v) of actively growing cells.…”

Section: Solvent Production From Hydrolysatementioning

confidence: 99%

“…The use of these unutilized agro-residues can serve as enormous resource for energy generation. Now-a-days, with several bio-re neries being established in the country due to the mandate shift towards cleaner bioenergy systems, the utilization of these agro lignocellulosic substrates can not only provide a sustainable solution for alternate energy resource but also help in better agricultural waste management [3].…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, large amount of research has been carried out to produce bio-butanol [12,13]. Bio-butanol is a superior fuel than bio-ethanol because of higher energy content and better air-to-fuel ratio.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of different lignocellulosic biomass for Solvent production by Clostridium beijerinckii strains

Dalal¹,

Joy²,

Das³

et al. 2021

Preprint

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The aim of the present study was to compare the effect of lignocellulosic sugar hydrolysate, obtained after acid pretreatment of different lignocellulosic biomass on production of solvents by two different Clostridium beijerinckii strains. C. beijerinckii C-01 produced solvents via the isopropanol-butanol-ethanol (IBE) pathway and C. beijerinckii B-17 via the acetone-butanol-ethanol (ABE). The hydrolysate was obtained after acid pretreatment of rice-husk, rice-straw and sugarcane-bagasse. Solvent production was observed from all the three substrates by both the strains. However, the highest solvent production of 9.2 gL− 1 similar to medium supplemented with commercial sugars was observed in sugarcane-bagasse hydrolysate by C. beijerinckii B-17. Degradation products i.e. 5-Hydroxy-methyl-furfural, levulinic acid, furfural and formic acid were found in different concentrations during acid pretreatments. The highest amount of formic acid (3.5 gL− 1), along with significant amounts of levulinic acid and furfural, was found to be present in rice-husk hydrolysate. The study shows that rice-straw and sugarcane-bagasse obtained after acid pretreatment are better substrates in comparison to rice-husk for butanol production by Clostridium beijerinckii strains.

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Co‐production of biobutanol and biomethane from distillers' grain waste by an integrated process

Wang,

Han

et al. 2023

J of Chemical Tech & Biotech

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BackgroundDistillers' grain waste (DGW) is the main by‐product in the Baijiu‐making process and its management remains a great challenge for the Baijiu industry. To address this issue, an integrated biobutanol and biomethane production process was established to obtain valuable products from DGW and reduce environmental pollution.ResultsIn the integrated process, DGW was hydrolyzed and detoxified to produce biobutanol, while the distillery waste derived from solvent extraction process was used for biomethane fermentation. After the optimization of overliming detoxification of DGW hydrolysate by artificial neural network and genetic algorithm, totals of 10.65 ± 0.07 g L−1 butanol and 4.51 ± 0.21 g L−1 isopropanol were attained with negligible amounts of acetone and ethanol (i.e. 0.14 ± 0.00 and 0.52 ± 0.06 g L−1, respectively). Anaerobic digestion of the distillery waste was efficient, and the chemical oxygen demand removal rate approached 92.3 ± 0.1% with a methane yield of 167.8 ± 1.2 mL g−1 TCODremoved. Moreover, the anaerobic digestion effluent could be recycled as a potential substitute for process water in biobutanol fermentation without having any negative effect on enzymatic hydrolysis of DGW, and the butanol production also reached 8.19 ± 0.29 g L−1.ConclusionThe present study provides a new idea for comprehensive utilization of DGW towards energy and resource recovery, without secondary pollution. © 2023 Society of Chemical Industry.

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Efficient isopropanol-butanol (IB) fermentation of rice straw hydrolysate by a newly isolated Clostridium beijerinckii strain C-01

Cited by 12 publications

References 37 publications

Comparative genome analysis of Clostridium beijerinckii strains isolated from pit mud of Chinese strong flavor baijiu ecosystem

Comparative genome analysis of Clostridium beijerinckii strains isolated from pit mud of Chinese strong flavor baijiu ecosystem

Comparison of different lignocellulosic biomass for Solvent production by Clostridium beijerinckii strains

Co‐production of biobutanol and biomethane from distillers' grain waste by an integrated process

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