Efficient isolation of subacute sclerosing panencephalitis virus from patient brains by reference to magnetic resonance and computed tomographic images

Ogura, Hisashi; Ayata, Minoru; Hayashi, Kaoru; Seto, Toshiyuki; Matsuoka, Osamu; Hattori, Hideji; Tanaka, Katsuyoshi; Tanaka, Kazuo; Takano, Yasuna; Murata, Ryosuke

doi:10.3109/13550289709029472

Cited by 29 publications

(20 citation statements)

References 23 publications

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“…Vero cells were cultured at 35 C in Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal calf serum and 4% newborn calf serum. The FrN and OcN viruses are sibling viruses of the SSPE virus Osaka-1 strain isolated by Vero cells from the frontal lobe and the occipital lobe, respectively, of a patient's brain (27). Vero cells persistently infected with these sibling viruses were subcultured with occasional feeding of fresh Vero cells and passaged every 4 to 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…The serial MR images of case 1 showed that SSPE lesions in the brain stem progressed rapidly in the frontal lobes and slowly in the occipital lobes. This finding was used in the selection of brain portions for virus isolation at autopsy, and two sibling viruses, Fr/V and Oc/V, of the Osaka-1 strain were successfully isolated by Vero cells (27). In this study, we biochemically and genetically characterized and compared the two sibling viruses to each other for understanding the expansion of SSPE virus in the infected brain, focusing on the M gene expression.…”

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confidence: 99%

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The Matrix Gene Expression of Subacute Sclerosing Panencephalitis (SSPE) Virus (Osaka‐1 Strain): A Comparison of Two Sibling Viruses Isolated from Different Lobes of an SSPE Brain

Ayata

Hayashi

Seto

et al. 1998

Microbiology and Immunology

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The Fr/V and Oc/V sibling viruses of the Osaka-1 strain of the subacute sclerosing panencephalitis (SSPE) virus were defective in cell-free virus production. By radioimmunoprecipitation assay, the matrix (M) protein was not detected in cells persistently infected with the Osaka-1 strain. This undetectable expression was consistent with the selective reduction of antibody response to the M protein in the patient from whom the Osaka-1 strain was isolated. The sequence of the M gene, however, predicted that the protein could be synthesized because the translational start and stop codons for the protein were not altered. Northern blot hybridization demonstrated the selective defect of the monocistronic mRNAs for the M protein and the phosphoprotein (P) together with the dominant presence of the P-M bicistronic mRNA. This absence of the M mRNA was further confirmed by primer extension analysis. Therefore, the undetectable expression of the M protein in the infected cells was proved to be caused by a transcriptional defect. The two sibling viruses, isolated from remote portions of an SSPE brain, were indistinguishable in their viral characters, including the M gene sequences, which indicates the possibility of clonal expansion of the strain in the brain.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

The Matrix Gene Expression of Subacute Sclerosing Panencephalitis (SSPE) Virus (Osaka‐1 Strain): A Comparison of Two Sibling Viruses Isolated from Different Lobes of an SSPE Brain

Ayata

Hayashi

Seto

et al. 1998

Microbiology and Immunology

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“…A Vero cell-adapted laboratory MV strain (Nagahata, genotype C1) and a field MV strain (OCU98-1P, genotype D3) isolated from peripheral blood lymphocytes of a patient with acute measles by cocultivation with B95a cells, and SSPE virus strains isolated from the brains of patients with SSPE have been described previously (5,36,44). In this study, we used three SSPE viruses (Osaka-1 Fr/V, Osaka-2 Fr/V, and Osaka-3 Oc/V) isolated from the brains of three patients.…”

Section: Cellsmentioning

confidence: 99%

“…These viruses are sibling viruses that were isolated with different cell lines (such as Vero or B95a cells) and from different regions of the brain (such as the frontal or occipital lobe). For isolation and designation of sibling viruses of each strain and detailed patient information, refer to (22,44).…”

Section: Cellsmentioning

confidence: 99%

“…On the other hand, SSPE viruses have generally been isolated from cells of non-lymphoid origin, such as Vero (16,25,35,37), BSC-1 (7), and primary human embryonic lung cells (58). We have tried to isolate our three SSPE viruses by cocultivation of brain cells from patients with SSPE with three different cell lines, Vero, B95a, and primary human embryonic lung cells (44). Although two of the three SSPE strains could be isolated by cocultivation with B95a cells, all strains were most successfully isolated by cocultivation with Vero cells.…”

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Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus

Ishida

Ayata

Shingai

et al. 2004

Microbiology and Immunology

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Measles virus is the causative agent of subacute sclerosing panencephalitis (SSPE). The viruses isolated from brain cells of patients with SSPE (called SSPE viruses) are defective in cell‐free virus production in vitro. To investigate the cell tropism of three strains of SSPE virus (Osaka‐1, Osaka‐2, Osaka‐3), SSPE virus‐infected cell cultures were treated with cytochalasin D to prepare virus‐like particles (CD‐VLPs). All CD‐VLPs formed syncytia after infection in CHO cells expressing CD150 but not in those expressing CD46. In addition, an antibody to CD46 did not block the infection of Vero cells by SSPE CD‐VLPs. The results were consistent with our previous suggestion that one or more unidentified receptors might be involved in the entry process. Infection with the CD‐VLPs from three SSPE strains was further examined in different human cell lines, including those of neural origin, and was found to induce syncytia in epithelial cells (HeLa and 293T) as well as neuroblastoma cells (IMR‐32 and SK‐N‐SH) with varying efficiency. SSPE CD‐VLPs also infected glioblastoma cells (A172) and astrocytoma cells (U‐251) but syncytial formation was rarely induced. These epithelial and neural cell lines were not permissive for the replication of wild‐type MV. Together with our previous observations, these results suggest that the cell entry receptor is the major factor determining the cell tropism of SSPE viruses. Further studies are necessary to identify other viral and/or cellular factors that might be involved in the replication of SSPE virus in specific neural cells and in the brain.

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Infections of the Central Nervous System

Schneider‐Schaulies¹,

Schneider‐Schaulies²,

Meulen³

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Efficient isolation of subacute sclerosing panencephalitis virus from patient brains by reference to magnetic resonance and computed tomographic images

Cited by 29 publications

References 23 publications

The Matrix Gene Expression of Subacute Sclerosing Panencephalitis (SSPE) Virus (Osaka‐1 Strain): A Comparison of Two Sibling Viruses Isolated from Different Lobes of an SSPE Brain

The Matrix Gene Expression of Subacute Sclerosing Panencephalitis (SSPE) Virus (Osaka‐1 Strain): A Comparison of Two Sibling Viruses Isolated from Different Lobes of an SSPE Brain

Infection of Different Cell Lines of Neural Origin with Subacute Sclerosing Panencephalitis (SSPE) Virus

Infections of the Central Nervous System

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