Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains

Ferrari, Michela; Hameleers, Lisanne; Stuart, Marc C. A.; Oerlemans, Marjolein M. P.; Vos, Paul de; Jurak, Edita; Walvoort, Marthe T. C.

doi:10.1016/j.carbpol.2021.118913

Cited by 8 publications

(3 citation statements)

References 31 publications

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“…We also determined that the mCGB was able to support the growth of the co-culture and monocultures. The EPS extraction was performed using the methods of Ferrari et al (2022) with modifications. Briefly, 9 mL of fresh medium was inoculated with 1 mL of co-culture and monoculture suspensions (OD 600 = 0.1) and incubated aerobically at 30°C.…”

Section: Growth and Exopolysaccharides (Eps) Productionmentioning

confidence: 99%

Enhanced probiotic potential of Lactobacillus kefiranofaciens OSU-BDGOA1 through co-culture with Kluyveromyces marxianus bdgo-ym6

et al. 2023

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IntroductionDue to the increasing consumer demand for the development and improvement of functional foods containing probiotics, new probiotic candidates need to be explored as well as novel means to enhance their beneficial effects. Lactobacillus kefiranofaciens OSU-BDGOA1 is a strain isolated from kefir grains that has demonstrated probiotic traits. This species is the main inhabitant of kefir grains and is responsible for the production of an exopolysaccharide (EPS) whit vast technological applications and potential bioactivities. Research has shown that interkingdom interactions of yeast and lactic acid bacteria can enhance metabolic activities and promote resistance to environmental stressors.MethodsComparative genomic analyses were performed to distinguish OSU-BDGOA1 from other strains of the same species, and the genome was mined to provide molecular evidence for relevant probiotic properties. We further assessed the cumulative effect on the probiotic properties of OSU-BDGOA1 and Kluyveromyces marxianus bdgo-ym6 yeast co-culture compared to monocultures.ResultsSurvival during simulated digestion assessed by the INFOGEST digestion model showed higher survival of OSU-BDGOA1 and bdgo-ym6 in co-culture. The adhesion to intestinal cells assessed with the Caco-2 intestinal cell model revealed enhanced adhesion of OSU-BDGOA1 in co-culture. The observed increase in survival during digestion could be associated with the increased production of EPS during the late exponential and early stationary phases of co-culture that, by enhancing co-aggregation between the yeast and the bacterium, protects the microorganisms from severe gastrointestinal conditions as observed by SEM images. Immune modulation and barrier function for recovery and prevention of flagellin-mediated inflammation by Salmonella Typhimurium heat-killed cells (HKSC) in Caco-2 cells were also measured. OSU-BDGOA1 in mono- and co-culture regulated inflammation through downregulation of pro-inflammatory cytokine expression and increased membrane barrier integrity assessed by TEER, FD4 permeability, and expression of tight junctions.DiscussionThe results of the study warrant further research into the application of co-cultures of yeast and LAB in functional probiotic products and the potential to increase EPS production by co-culture strategies.

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Section: Growth and Exopolysaccharides (Eps) Productionmentioning

confidence: 99%

Enhanced probiotic potential of Lactobacillus kefiranofaciens OSU-BDGOA1 through co-culture with Kluyveromyces marxianus bdgo-ym6

et al. 2023

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“…A cheap medium with a high concentration of glucose, yeast extract and soy peptone was investigated to achieve suitable biomass yields and productivities, thereby shortening process time. However, the presence of polysaccharide contaminants in complex nitrogen sources complicates the purification of potentially bioactive compounds, such as exopolysaccharides produced by lactobacilli, and the characterization of their structure (Ferrari et al, 2022). To define a simpler medium and an industrially feasible purification strategy, the effect of lower concentrations of complex nitrogen sources (and glucose) was evaluated in the Frontiers in Bioengineering and Biotechnology frontiersin.org same experimental set-up demonstrating, unexpectedly, that the reduction did not affect the titer of viable cells and of EPS produced.…”

Section: Discussionmentioning

confidence: 99%

Optimization of growth of Levilactobacillus brevis SP 48 and in vitro evaluation of the effect of viable cells and high molecular weight potential postbiotics on Helicobacter pylori

Cimini

D’ambrosio²,

Stellavato³

et al. 2022

Front. Bioeng. Biotechnol.

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Several Levilactobacillus brevis strains have the potential to be used as probiotics since they provide health benefits due to the interaction of live cells, and of their secreted products, with the host (tissues). Therefore, the development of simple fermentation processes that improve cell viability to reduce industrial production costs, and at the same time the characterization and biological evaluation of cell-free postbiotics that can further promote application, are of great interest. In the present study, small scale batch fermentations on semi defined media, deprived of animal derived raw materials, were used to optimize growth of L. brevis SP48, reaching 1.2 ± 0.4 × 1010 CFU/ml of viable cells after 16 h of growth. Displacement, competition, and inhibition assays compared the effect, on Helicobacter pylori, of L. brevis cells to that of its partially purified potentially postbiotic fraction rich in exopolysaccharides and proteins. The expression of pro and anti-inflammatory biochemical markers indicated that both samples activated antimicrobial defenses and innate immunity in a gastric model. Moreover, these compounds also acted as modulators of the inflammatory response in a gut in vitro model. These data demonstrate that the high molecular weight compounds secreted by L. brevis SP48 can contrast H. pylori and reduce inflammation related to intestinal bowel disease, potentially overcoming issues related to the preservation of probiotic viability.

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“…For the characterization of A. muciniphila EPS, we mainly focused on the EPS found in the supernatant of the cultures. However, a recent study including four bifidobacterial strains showed distinct structural differences in entirely excreted EPS compared to membrane-associated EPS (Ferrari et al 2022). In our study, FTIR analysis indicated that extracellular carbohydrates are present in the pellet of A. muciniphila, including the production of sialic acids in conditions containing GlcNAc and glucose.…”

Section: Dietary Polyphenols Were Found To Induce Capsular Polysaccha...mentioning

confidence: 97%

A rising star : A comprehensive approach to Akkermansia muciniphila ecosystems, interactions and applications

Geerlings¹

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General introduction and thesis outlineAkkermansia muciniphila is a fascinating intestinal symbiont, which has received considerable attention over the last two decades due to its mechanisms of action to improve host health. In this thesis, A. muciniphila plays a main role in all the chapters. A. muciniphila Muc T was isolated in 2004 at the laboratory of Microbiology at Wageningen University (Derrien et al. 2004). It is a Gram-negative bacterium belonging to the Verrucomicrobiota (formerly Verrucomicrobia) phylum. In the human gut, A. muciniphila is currently the only representative of this phylum and present in high numbers with an abundance ranging from 0.5-5% (Cani and de Vos 2017). The name A. muciniphila was derived from Dr. Antoon Akkermans, who was leading the Microbial Ecology group within the Laboratory of Microbiology at the time of isolation, shortly before his retirement. The isolation was performed on the fecal sample of a healthy individual using purified mucin as the sole source of carbon, nitrogen, and energy (Derrien et al. 2004, Belzer and de Vos 2012. The genome of A. muciniphila Muc T is composed of one circular chromosome of 2.7 Mbp coding for 2,176 protein-coding genes and is in terms of function mainly dedicated to mucus degradation (van Passel et al. 2011). Here an introduction is provided into the role of A. muciniphila in host health and other functions of this bacterium. In addition, we will introduce different models that may be used to study its performance. A. muciniphila involvement in human healthExtensive studies have been performed on the correlation between the gut microbiota and health and disease (de Vos et al. 2022). The gut microbiota composition was found to be strongly correlated to several diseases, including pre-diabetes, type 2 diabetes, obesity, non-alcoholic fatty liver disease and liver cirrhosis (

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Efficient isolation of membrane-associated exopolysaccharides of four commercial bifidobacterial strains

Cited by 8 publications

References 31 publications

Enhanced probiotic potential of Lactobacillus kefiranofaciens OSU-BDGOA1 through co-culture with Kluyveromyces marxianus bdgo-ym6

Enhanced probiotic potential of Lactobacillus kefiranofaciens OSU-BDGOA1 through co-culture with Kluyveromyces marxianus bdgo-ym6

Optimization of growth of Levilactobacillus brevis SP 48 and in vitro evaluation of the effect of viable cells and high molecular weight potential postbiotics on Helicobacter pylori

A rising star : A comprehensive approach to Akkermansia muciniphila ecosystems, interactions and applications

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