Efficient Isolation of
            <b>
              <i>Campylobacter upsaliensis</i>
              from Stools
            </b>

Lastovica, Albert J.; Roux, E. Le

doi:10.1128/jcm.39.11.4222-4223.2001

Cited by 83 publications

(110 citation statements)

References 5 publications

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“…However, this study did not perform colonic biopsies to ascertain if the H. cinaedi-positive monkeys had histological evidence of chronic colitis, a common finding in rhesus monkeys (Fernandez et al, 2002). Isolation of other novel helicobacters from inflamed colons of rhesus monkeys and cotton-top tamarins is also consistent with the increasing recognition of enteric helicobacters in children with gastroenteritis who reside in developing countries (Fox et al, 2001b;Lastovica & le Roux, 2000;Saunders et al, 1999) and the identity of enteric helicobacters in the lower bowel of children with inflammatory bowel disease (Thomson et al, 2011;Zhang et al, 2006). Our findings in this study are consistent with previous NHP studies, in that we identified Helicobacter jaachi sp.…”

Section: Discussionsupporting

confidence: 82%

Isolation and characterization of a novel Helicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus)

Shen

Feng

Sheh

et al. 2015

Journal of Medical Microbiology

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Isolation and characterization of a novelHelicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus) Purpose-bred common marmosets from domestic sources housed in a US research facility, and used in multiple drug discovery programmes, were noted to have a high incidence of spontaneous inflammatory bowel disease and sporadic cholecystitis and cholangiohepatitis. Inflammatory infiltrates increased in incidence and severity with age. Because Helicobacter spp. have been linked to gastrointestinal diseases, samples from the gastrointestinal tracts of 39 marmosets were screened for Helicobacter spp. by culture and PCR. Helicobacter spp. were frequently detected in marmosets; 28.2 % of the marmosets were positive for a proposed novel species, Helicobacter jaachi sp. nov., by culture, and 48.7 % were positive by Helicobacter genus-specific PCR. Seventeen strains of Helicobacter sp. from 11 marmosets were cultured from various gastrointestinal sites. Older animals (age 6-11 years) had a higher helicobacter prevalence rate (57.1 %) compared with younger animals (age 3-5 years), which had a 27.2 % prevalence rate. Cells of H. jaachi sp. nov. were catalase, urease and oxidase positive and had fusiform morphology, with periplasmic fibres and multiple bipolar, sheathed flagella. All isolates had similar 16S and 23S rRNA sequences, which clustered as representatives of a novel Helicobacter species closely related to 'Helicobacter sanguini' (97 %), a species isolated from cotton-top tamarins and 'Helicobacter callitrichis' (96 %) isolated previously from the faeces of common marmosets. The whole genome sequence of one of the liver isolates, H. jaachi sp. nov. MIT 09-6949 T , had a 1.9 Mb genome length with a 41 mol% DNA G+C content. The type strain of Helicobacter jaachi sp. nov., MIT 09-6949 T , has been deposited in the BCCM/LMG Bacteria Collection as LMG 28613 T . These findings add to the increasing number of animal species with gastrointestinal disease in which novel enterohepatic Helicobacter spp. have been isolated. INTRODUCTIONThe common marmoset (Callithrix jacchus) has been utilized as a non-human primate model for nonclinical pharmaceutical development (Orsi et al., 2011). The common marmoset's importance as a model in biomedical research will be enhanced with the recent publication of its genome (Worley et al., 2014). An illustration of the marmoset's usefulness is its use in neuroscience research and genome editing technology (Kishi et al., 2014). A small common marmoset colony was established at GlaxoSmithKline to serve as a preclinical model for drug discovery and development. Common marmosets develop diet-induced obesity phenotypes when placed on high fat diets and were employed in studies to help develop anti-obesity compounds (Tardif et al., 2009). Over the course of several years, complete necropsies were performed on 46 marmosets originating from this colony. The spectrum of spontaneous pathology documented was similar to that reported in the literature for common marmoset...

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Section: Discussionsupporting

confidence: 82%

Isolation and characterization of a novel Helicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus)

Shen

Feng

Sheh

et al. 2015

Journal of Medical Microbiology

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“…Filtrates are plated onto antibiotic-free blood agar plates and incubated in an H 2 -enriched atmosphere (Lastovica & le Roux, 2000). The authors reported a 16% prevalence of multiple Campylobacter species and Helicobacter species based on primary isolation and biochemical characterization.…”

Section: Recent Experimental Results and Epidemiological Studies Suggmentioning

confidence: 99%

“…The goal of this study was to determine whether Helicobacter species could be isolated from the gastrointestinal tract and/or liver of prairie dogs, which are used as models to study gallbladder physiology because of the similarities of prairie dog and human bile gallstone composition. It is also recognized that Helicobacter species co-colonize with a variety of Campylobacter species in the intestines of humans, dogs and cats (Fox et al, 1995;Lastovica & le Roux, 2000;Rossi et al, 2008;Shen et al, 2001). We therefore asked whether Campylobacter species could also be identified in the gastrointestinal tract of prairie dogs.…”

Section: Introductionmentioning

confidence: 99%

Helicobacter marmotae and novel Helicobacter and Campylobacter species isolated from the livers and intestines of prairie dogs

Beisele

Shen

Parry

et al. 2011

Journal of Medical Microbiology

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Prairie dogs (Cynomys ludovicianus) are used to study the aetiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. Epidemiological and experimental studies have suggested a connection between infection with Helicobacter species and cholesterol cholelithiasis, cholecystis and gallbladder cancer. Ten of the 34 prairie dogs in this study had positive Helicobacter species identified by PCR using Helicobacter genusspecific primers. Ten of 34 prairie dogs had positive Campylobacter species identified in the intestine by PCR with Campylobacter genus-specific primers. Six Helicobacter sp. isolates and three Campylobacter sp. isolates were identified taxonomically by 16S rRNA gene analysis. The prairie dog helicobacters fell into three clusters adjacent to Helicobacter marmotae. On the basis of 16S rRNA gene sequence analysis, three strains in two adjacent clusters were included in the species H. marmotae. Three strains were only 97.1 % similar to the sequence of H. marmotae and can be considered a novel species with the provisional designation Helicobacter sp. Prairie Dog 3. The prairie dog campylobacters formed a single novel cluster and represent a novel Campylobacter sp. with the provisional designation Campylobacter sp. Prairie Dog. They branched with Campylobacter cuniculorum at 96.3 % similarity and had the greatest sequence similarity to Campylobacter helveticus at 97.1 % similarity. Whether H. marmotae or the novel Helicobacter sp. and Campylobacter sp. identified in prairie dogs play a role in cholesterol gallstones or hepatobiliary disease requires further studies.

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“…The other campylobacters such as Campylobacter lari, Campylobacter upsaliensis, and Campylobacter fetus are more seldomly found, but vary depending on different regions of the world. C. upsaliensis, for example, is frequently isolated in South Africa [69].…”

Section: Enteric Infectionmentioning

confidence: 99%

Campylobacter

et al. 2005

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-Species within the genus, Campylobacter, have emerged over the last three decades as significant clinical pathogens, particularly of human public health concern, where the majority of acute bacterial enteritis in the Western world is due to these organisms. Of particular concern are the species, C. jejuni and C. coli, which are responsible for most of these gastrointestinal-related infections. Although these organisms have already emerged as causative agents of zoonoses, several aspects of their epidemiology and pathophysiology are only beginning to emerge. Trends in increasing antibiotic resistance are beginning to emerge with oral antibiotics, which may be the drug of choice for when it is necessary to intervene chemotherapeutically. This review wishes to examine (i) emerging clinical aspects of the disease, such as Guillain Barré syndrome (GBS), (ii) the association between these organisms and poultry as a natural host, (iii) environmental aspects of Campylobacter epidemiology, (iv) the emergence of atypical campylobacters (v) emerging trends in antibiotic resistance, (vi) adoption of modern methods for the detection of campylobacters.

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Efficient Isolation of Campylobacter upsaliensis from Stools

Cited by 83 publications

References 5 publications

Isolation and characterization of a novel Helicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus)

Isolation and characterization of a novel Helicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus)

Helicobacter marmotae and novel Helicobacter and Campylobacter species isolated from the livers and intestines of prairie dogs

Campylobacter

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