Efficient Isolation and Gene Expression Profiling of Small Numbers of Neural Crest Stem Cells and Developing Schwann Cells

Buchstaller, Johanna; Sommer, Lukas; Bodmer, Matthias; Hoffmann, Reinhard; Suter, Ueli; Mantei, Ned

doi:10.1523/jneurosci.4083-03.2004

Cited by 86 publications

(101 citation statements)

References 80 publications

(99 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results relating to NCCs markers are in agreement with those of Buchstaller et al (2004), who used a different approach to screen a small population of NCCs in early and late stages of transgenic mouse development.…”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Gene expression profiling identifies eleven DNA repair genes down-regulated during mouse neural crest cell migration

et al. 2011

View full text Add to dashboard Cite

Neural Crest Cells (NCCs) are transient multipotent migratory cells that derive from the embryonic neural crest which is itself derived from the margin of the neural tube. DNA repair genes are expressed in the early stages of mammalian development to reduce possible replication errors and genotoxic damage. Some birth defects and cancers are due to inappropriate or defective DNA repair machinery, indicating that the proper functioning of DNA repair genes in the early stages of fetal development is essential for maintaining DNA integrity. We performed a genome-wide expression analysis combining laser capture microdissection (LCM) and highdensity oligo-microarray of murine NCCs at pre-migratory embryonic days 8.5 (E8.5), and at E13.5, as well as on neural crest-derived cells from the adrenal medulla at postnatal day 90. We found 11 genes involved in DNA repair activity (response to DNA damage stimulus, DNA damage checkpoint, base-excision repair, mismatch repair), over-expressed in the early stages of mouse embryo development. Expression of these 11 genes was very low or undetectable in the differentiated adrenal medulla of the adult mouse. Amongst the 11 genes, 6 had not been previously reported as being over-expressed during mouse embryonic development. High expression of DNA repair genes in enriched NCCs during early embryonic development may contribute to maintaining DNA integrity whilst failure of some of these genes may be associated with the onset of genetic disease and cancer. Our model of enriched murine NCCs and neural crest-derived cells can be used to elucidate the key roles of genes during normal embryonic development and in cancer pathogenesis.

show abstract

Section: Discussionsupporting

confidence: 90%

“…Previously, the expression of DNA repair genes in mouse embryonic NCCs was studied, in part, by a single-gene approach and by low-density microarray (Buchstaller et al 2004). Here instead, we used LCM and a high-density oligo-microarray to characterize NCCs.…”

Section: Introductionmentioning

confidence: 99%

Gene expression profiling identifies eleven DNA repair genes down-regulated during mouse neural crest cell migration

et al. 2011

View full text Add to dashboard Cite

show abstract

“…DRG explant cultures of ErbB3-deficient embryos reflect the migration impairment of the Schwann cell precursor (Riethmacher et al, 1997) and therefore represent an ideal model for expression analyses of the genes that are involved in migration when compared with those of wild-type cultures. Our microarray analyses revealed a prominent differential expression of ECM molecules, which is independently supported by microarray data that has been collected using isolated neural crest stem cells and Schwann cell precursors (Buchstaller et al, 2004). The highly differential expression of ECM molecules coincides with axonal growth and the on-going process of Schwann cell precursor migration along the axons, suggesting a potential involvement of such ECM components in these processes.…”

Section: Discussionsupporting

confidence: 79%

Promotion of periostin expression contributes to the migration of Schwann cells

Sonnenberg-Riethmacher

Miehe

Riethmacher

2015

Journal of Cell Science

View full text Add to dashboard Cite

Neuregulin ligands and their ErbB receptors are important for the development of Schwann cells, the glial cells of the peripheral nervous system (PNS). ErbB3 deficiency is characterized by a complete loss of Schwann cells along axons of the peripheral nerves, impaired fasciculation and neuronal cell death. We performed comparative gene expression analysis of dorsal root ganglia (DRG) explant cultures from ErbB3-deficient and wild-type mice in order to identify genes that are involved in Schwann cell development and migration. The extracellular matrix (ECM) gene periostin was found to exhibit the most prominent down regulation in ErbB3-deficient DRG. Expression analysis revealed that the periostin-expressing cell population in the PNS corresponds to Schwann cell precursors and Schwann cells, and is particularly high in migratory Schwann cells. Furthermore, stimulation of Schwann cells with neuregulin-1 (NRG1) or transforming growth factor β (TGFβ-1) resulted in an upregulation of periostin expression. Interestingly, DRG explant cultures of periostindeficient mice revealed a significant reduction of the number of migrating Schwann cells. These data demonstrate that the expression of periostin is stimulated by ErbB ligand NRG1 and influences the migration of Schwann cell precursors.

show abstract

“…In the case of myelinating cells this involves downregulation of a large number of genes related to myelination (reviewed in Jessen and Mirsky, 2005;Mirsky et al, 2008;Scherer and Salzer, 2001). This includes enzymes that provide for cholesterol synthesis, structural proteins such as P 0 , myelin basic protein (MBP), and membrane associated proteins such as myelin associated glycoprotein (MAG) and periaxin (Buchstaller et al, 2004;D'Antonio et al, 2006;Leblanc et al, 2005;Nagarajan et al, 2001Nagarajan et al, , 2002Verheijen et al, 2003). This switch-off is accompanied by the activation of another group of molecules, most of which are normally found on immature cells prior to myelination.…”

Section: Schwann Cell Dedifferentiation During Wallerian Degenerationmentioning

confidence: 99%

Negative regulation of myelination: Relevance for development, injury, and demyelinating disease

Jessen

Mirsky

2008

Glia

446

409

View full text Add to dashboard Cite

Dedifferentiation of myelinating Schwann cells is a key feature of nerve injury and demyelinating neuropathies. We review recent evidence that this dedifferentiation depends on activation of specific intracellular signaling molecules that drive the dedifferentiation program. In particular, we discuss the idea that Schwann cells contain negative transcriptional regulators of myelination that functionally complement positive regulators such as Krox-20, and that myelination is therefore determined by a balance between two opposing transcriptional programs. Negative transcriptional regulators should be expressed prior to myelination, downregulated as myelination starts but reactivated as Schwann cells dedifferentiate following injury. The clearest evidence for a factor that works in this way relates to c-Jun, while other factors may include Notch, Sox-2, Pax-3, Id2, Krox-24, and Egr-3. The role of cell-cell signals such as neuregulin-1 and cytoplasmic signaling pathways such as the extracellular-related kinase (ERK)1/2 pathway in promoting dedifferentiation of myelinating cells is also discussed. We also review evidence that neurotrophin 3 (NT3), purinergic signaling, and nitric oxide synthase are involved in suppressing myelination. The realization that myelination is subject to negative as well as positive controls contributes significantly to the understanding of Schwann cell plasticity. Negative regulators are likely to have a major role during injury, because they promote the transformation of damaged nerves to an environment that fosters neuronal survival and axonal regrowth. In neuropathies, however, activation of these pathways is likely to be harmful because they may be key contributors to demyelination, a situation which would open new routes for clinical intervention. V V C 2008 Wiley-Liss, Inc.

show abstract

Efficient Isolation and Gene Expression Profiling of Small Numbers of Neural Crest Stem Cells and Developing Schwann Cells

Cited by 86 publications

References 80 publications

Gene expression profiling identifies eleven DNA repair genes down-regulated during mouse neural crest cell migration

Gene expression profiling identifies eleven DNA repair genes down-regulated during mouse neural crest cell migration

Promotion of periostin expression contributes to the migration of Schwann cells

Negative regulation of myelination: Relevance for development, injury, and demyelinating disease

Contact Info

Product

Resources

About