Efficient Intracellular Delivery of RNase A Using DNA Origami Carriers

Zhao, Shuai; Duan, Fangyuan; Liu, Shaoli; Wu, Tiantian; Shang, Yingxu; Tian, Run; Li, Jianbing; Wang, Zhen‐Gang; Jiang, Qiao; Ding, Baoquan

doi:10.1021/acsami.8b21724

Cited by 81 publications

(70 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, RI protection for the host cell can be overcome by protein engineering by means of conjugation to nanomaterials or oligomerization [46,91,94,95]. This strategy has, indeed, been proposed for targeting cancer cells and other chemotherapies [46,65,96]. However, one of the most common and challenging obstacles to achieve a full cytotoxic effect is insufficient cellular uptake [46,85].…”

Section: Discussionmentioning

confidence: 99%

Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa

et al. 2020

View full text Add to dashboard Cite

Current treatments against bacterial infections have severe limitations, mainly due to the emergence of resistance to conventional antibiotics. In the specific case of Pseudomonas aeruginosa strains, they have shown a number of resistance mechanisms to counter most antibiotics. Human secretory RNases from the RNase A superfamily are proteins involved in a wide variety of biological functions, including antimicrobial activity. The objective of this work was to explore the intracellular antimicrobial action of an RNase 3/1 hybrid protein that combines RNase 1 high catalytic and RNase 3 bactericidal activities. To achieve this, we immobilized the RNase 3/1 hybrid on Polyetheramine (PEA)-modified magnetite nanoparticles (MNPs). The obtained nanobioconjugates were tested in macrophage-derived THP-1 cells infected with Pseudomonas aeruginosa PAO1. The obtained results show high antimicrobial activity of the functionalized hybrid protein (MNP-RNase 3/1) against the intracellular growth of P. aeruginosa of the functionalized hybrid protein. Moreover, the immobilization of RNase 3/1 enhances its antimicrobial and cell-penetrating activities without generating any significant cell damage. Considering the observed antibacterial activity, the immobilization of the RNase A superfamily and derived proteins represents an innovative approach for the development of new strategies using nanoparticles to deliver antimicrobials that counteract P. aeruginosa intracellular infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa

et al. 2020

View full text Add to dashboard Cite

show abstract

“…[28] Therei sawide varietyo fD NA-based vesselsf or enzymatic cargos such as cascaden anoreactors, [29] tubularh osts, [30] reconfigurable vaults andc apsules ( Figure 1a,top panel), [31,32] andvarious DNAcages (Figure1a, bottom panel) [33,34] as well as nanosheets forn ucleased elivery. [35] Theset ypes of (multi-)enzymes ystems andv ehiclesw ithe nzymatic payloadsh ave been reviewed in Refs. [26,36].Itisnoteworthy that harnessing DNAtemplates forprotein assembly mayhaveratherintriguinga nd unconventional implementationsi nt ailoredp rotein design,a sd emonstratedb yR osiere tal.…”

Section: Biomedical Applications Of Dna Nanostructuresmentioning

confidence: 99%

Challenges and Perspectives of DNA Nanostructures in Biomedicine

2020

View full text Add to dashboard Cite

DNA nanotechnology holds substantial promise for future biomedical engineering and the development of novel therapies and diagnostic assays. The subnanometer‐level addressability of DNA nanostructures allows for their precise and tailored modification with numerous chemical and biological entities, which makes them fit to serve as accurate diagnostic tools and multifunctional carriers for targeted drug delivery. The absolute control over shape, size, and function enables the fabrication of tailored and dynamic devices, such as DNA nanorobots that can execute programmed tasks and react to various external stimuli. Even though several studies have demonstrated the successful operation of various biomedical DNA nanostructures both in vitro and in vivo, major obstacles remain on the path to real‐world applications of DNA‐based nanomedicine. Here, we summarize the current status of the field and the main implementations of biomedical DNA nanostructures. In particular, we focus on open challenges and untackled issues and discuss possible solutions.

show abstract

“…Li et al designed a programming rectangular DNA origami nanorobot (20 nm × 30 nm), which effectively penetrated ovarian cancer cells when loaded with adriamycin [ 76 ]. DNA origami nanorobots were also reported to successfully deliver thrombin or ribonuclease (RNase) A molecules to the target cells [ 77 , 78 ]. Capture strands (blue) linked to cytotoxic protein RNase A molecules were extended on the surface of rectangular DNA origami template, and aptamers targeting cancer cells are also integrated to enhance the targeting effect ( Figure 4 B) [ 78 ].…”

Section: Other Types Of Micro/nanorobotsmentioning

confidence: 99%

Micro/Nanorobot: A Promising Targeted Drug Delivery System

Chen

et al. 2020

Pharmaceutics

100

View full text Add to dashboard Cite

Micro/nanorobot, as a research field, has attracted interest in recent years. It has great potential in medical treatment, as it can be applied in targeted drug delivery, surgical operation, disease diagnosis, etc. Differently from traditional drug delivery, which relies on blood circulation to reach the target, the designed micro/nanorobots can move autonomously, which makes it possible to deliver drugs to the hard-to-reach areas. Micro/nanorobots were driven by exogenous power (magnetic fields, light energy, acoustic fields, electric fields, etc.) or endogenous power (chemical reaction energy). Cell-based micro/nanorobots and DNA origami without autonomous movement ability were also introduced in this article. Although micro/nanorobots have excellent prospects, the current research is mainly based on in vitro experiments; in vivo research is still in its infancy. Further biological experiments are required to verify in vivo drug delivery effects of micro/nanorobots. This paper mainly discusses the research status, challenges, and future development of micro/nanorobots.

show abstract

Efficient Intracellular Delivery of RNase A Using DNA Origami Carriers

Cited by 81 publications

References 30 publications

Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa

Magnetite Nanoparticles Functionalized with RNases against Intracellular Infection of Pseudomonas aeruginosa

Challenges and Perspectives of DNA Nanostructures in Biomedicine

Micro/Nanorobot: A Promising Targeted Drug Delivery System

Contact Info

Product

Resources

About