Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells

Kunzmann, Andrea; Andersson, Britta; Vogt, Carmen; Feliu, Neus; Ye, Fei; Gabrielsson, Susanne; Toprak, Muhammet S.; Buerki-Thurnherr, Tina; Laurent, Sophie; Vahter, Marie; Krug, Harald F.; Muhammed, Mamoun; Scheynius, Annika; Fadeel, Bengt

doi:10.1016/j.taap.2011.03.011

Cited by 176 publications

(108 citation statements)

References 142 publications

(102 reference statements)

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“…studies have indicated that dendritic cells (DCs) and macrophages, which are especially abundant in the mucosae and the skin, not only undergo oxidative stress, necrosis, and apoptosis but also overexpress T lymphocyte-stimulating proteins, such as major histocompatibility complex class II (MHC-II), CD80, and CD86, and secrete several proinflammatory cytokines and chemokines when they are treated with a variety of NPs (mesoporous silica NPs [SiO 2 NPs], TiO 2 NPs, and silica-coated iron dioxide and TiO 2 NPs) (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). Consistent with that finding, in mouse models, various modified silica NPs increased the efficacy with which DCs induced the differentiation of gamma interferon (IFN-␥)-producing CD8 ϩ T lymphocytes (Tc1), a phenotype linked with tissue inflammatory diseases, such as delayed-type hypersensitivity (DTH), characterized by macrophage and polymorphonuclear leukocyte (PMN) infiltration (18).…”

mentioning

confidence: 99%

Combined Action of Human Commensal Bacteria and Amorphous Silica Nanoparticles on the Viability and Immune Responses of Dendritic Cells

Malachin

Lubian

Mancin

et al. 2017

Clin Vaccine Immunol

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Dendritic cells (DCs) regulate the host-microbe balance in the gut and skin, tissues likely exposed to nanoparticles (NPs) present in drugs, food, and cosmetics. We analyzed the viability and the activation of DCs incubated with extracellular media (EMs) obtained from cultures of commensal bacteria (Escherichia coli, Staphylococcus epidermidis) or pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) in the presence of amorphous silica nanoparticles (SiO 2 NPs). EMs and NPs synergistically increased the levels of cytotoxicity and cytokine production, with different nanoparticle dose-response characteristics being found, depending on the bacterial species. E. coli and S. epidermidis EMs plus NPs at nontoxic doses stimulated the secretion of interleukin-1␤ (IL-1␤), IL-12, IL-10, and IL-6, while E. coli and S. epidermidis EMs plus NPs at toxic doses stimulated the secretion of gamma interferon (IFN-␥), tumor necrosis factor alpha (TNF-␣), IL-4, and IL-5. On the contrary, S. aureus and P. aeruginosa EMs induced cytokines only when they were combined with NPs at toxic concentrations. The induction of maturation markers (CD86, CD80, CD83, intercellular adhesion molecule 1, and major histocompatibility complex class II) by commensal bacteria but not by pathogenic ones was improved in the presence of noncytotoxic SiO 2 NP doses. DCs consistently supported the proliferation and differentiation of CD4 ϩ and CD8 ϩ T cells secreting IFN-␥ and IL-17A. The synergistic induction of CD86 was due to nonprotein molecules present in the EMs from all bacteria tested. At variance with this finding, the synergistic induction of IL-1␤ was prevalently mediated by proteins in the case of E. coli EMs and by nonproteins in the case of S. epidermidis EMs. A bacterial costimulus did not act on DCs after adsorption on SiO 2 NPs but rather acted as an independent agonist. The inflammatory and immune actions of DCs stimulated by commensal bacterial agonists might be altered by the simultaneous exposure to engineered or environmental NPs.

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mentioning

confidence: 99%

Combined Action of Human Commensal Bacteria and Amorphous Silica Nanoparticles on the Viability and Immune Responses of Dendritic Cells

Malachin

Lubian

Mancin

et al. 2017

Clin Vaccine Immunol

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“…29 Moreover, silica-coated SPIO nanoparticles, rather than dextran-coated nanoparticles, displayed dose-dependent cytotoxicity. 30 In our study, none of the nanoparticle formulations affected the viability of the macrophages ( Figure 3B). Our dextran-coated nanoparticles are safer than nanoparticles coated with aminosiline and silica.…”

Section: Discussionmentioning

confidence: 49%

“…Our dextran-coated nanoparticles are safer than nanoparticles coated with aminosiline and silica. 29,30 The different charge and coating states of the surface potential did not influence the cell viability of cultured macrophages in the four types of SPIO and USPIO contrast agents that we used.…”

Section: Discussionmentioning

confidence: 89%

Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

Saito¹,

Tsugeno²,

Koto³

et al. 2012

IJN

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Purpose: Magnetic resonance imaging (MRI) using contrast agents like superparamagnetic iron oxide (SPIO) is an extremely versatile technique to diagnose diseases and to monitor treatment. This study tested the relative importance of particle size and surface coating for the optimization of MRI contrast and labeling efficiency of macrophages migrating to remote inflammation sites. Materials and methods:We tested four SPIO and ultrasmall superparamagnetic iron oxide (USPIO), alkali-treated dextran magnetite (ATDM) with particle sizes of 28 and 74 nm, and carboxymethyl dextran magnetite (CMDM) with particle sizes of 28 and 72 nm. Mouse macrophage RAW264 cells were incubated with SPIOs and USPIOs, and the labeling efficiency of the cells was determined by the percentage of Berlin blue-stained cells and by measuring T 2 relaxation times with 11.7-T MRI. We used trypan blue staining to measure cell viability. Results: Analysis of the properties of the nanoparticles revealed that ATDM-coated 74 nm particles have a lower T 2 relaxation time than the others, translating into a higher ability of MRI negative contrast agent. Among the other three candidates, CMDM-coated particles showed the highest T 2 relaxation time once internalized by macrophages. Regarding labeling efficiency, ATDM coating resulted in a cellular uptake higher than CMDM coating, independent of nanoparticle size. None of these particle formulations affected macrophage viability. Conclusion: This study suggests that coating is more critical than size to optimize the SPIO labeling of macrophages. Among the formulations tested in this study, the best MRI contrast and labeling efficiency are expected with ATDM-coated 74 nm nanoparticles.

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“…In many cases, the toxic effects are dose dependent and related to specific nanoparticle composition aspects. 119 Composition has been shown to be clearly related to potential adverse effects and in general for their performance as cell labelling agent for a large variety of nanoparticle as also illustrated in the section on nanoparticle composition.…”

Section: Effects Of Nanoparticle Sizementioning

confidence: 99%

Nanoparticles and clinically applicable cell tracking

Bernsen

Guenoun

Tiel

et al. 2015

BJR

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In vivo cell tracking has emerged as a much sought after tool for design and monitoring of cell-based treatment strategies. Various techniques are available for pre-clinical animal studies, from which much has been learned and still can be learned. However, there is also a need for clinically translatable techniques. Central to in vivo cell imaging is labelling of cells with agents that can give rise to signals in vivo, that can be detected and measured non-invasively. The current imaging technology of choice for clinical translation is MRI in combination with labelling of cells with magnetic agents. The main challenge encountered during the cell labelling procedure is to efficiently incorporate the label into the cell, such that the labelled cells can be imaged at high sensitivity for prolonged periods of time, without the labelling process affecting the functionality of the cells. In this respect, nanoparticles offer attractive features since their structure and chemical properties can be modified to facilitate cellular incorporation and because they can carry a high payload of the relevant label into cells. While these technologies have already been applied in clinical trials and have increased the understanding of cell-based therapy mechanism, many challenges are still faced.

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Efficient internalization of silica-coated iron oxide nanoparticles of different sizes by primary human macrophages and dendritic cells

Cited by 176 publications

References 142 publications

Combined Action of Human Commensal Bacteria and Amorphous Silica Nanoparticles on the Viability and Immune Responses of Dendritic Cells

Combined Action of Human Commensal Bacteria and Amorphous Silica Nanoparticles on the Viability and Immune Responses of Dendritic Cells

Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

Nanoparticles and clinically applicable cell tracking

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