Efficient indirect induction of protocorm-like bodies and shoot proliferation using field-grown axillary buds of a Lycaste hybrid

Abstract

This work presents a rapid and reliable micropropagation method for a Lycaste hybrid using a fieldgrown axillary bud culture system. Intact buds (2-4 mm in length) were excised from a mature pseudobulb and were cultured in half-strength MS basal medium, which was supplemented with 0.5 mg l -1 benzyladenine (BA), 1.0 mg l -1 thidiazuron (TDZ) and 2% (w/v) sucrose. After 2 months, the calli exhibited vigorous growth and eventually turned green, forming protocorm-like bodies (PLBs) originating in the surface of each callus. The results of this work reveal that the combination of 0.5 mg l -1 BA and 1.0 mg l -1 TDZ treatments was highly effective in indirectly multiplying shoots from callus-PLB mixed explants, which yielded up to 400 shoots in the fourth time subcultures (within 24 weeks). Histological observations showed the apical meristem of adventitious bud is based on a longitudinal section of a callus sample. Histological and scanning electronic microscopy also indicated that PLBs derived from calli could be regarded as organogenesis but not somatic embryogenesis. Shoots with a length of around 2-3 cm generated in vitro were excised and cultured in MS medium supplemented with 0.5 mg l -1 IBA exhibited the best rooting response (78.3%), and an average of 1.8 roots per explant was produced within 4 weeks.

Direct induction of protocorm-like bodies from shoot tips, plantlet formation, and clonal fidelity analysis in Anthurium andreanum cv. CanCan

“…It has been reported that AC remove light in the media and provides a suitable physical environment for the rhizosphere and facilitates rooting in A. andreanum (Gantait et al 2008). In accordance to our report on this bi-phasic (shoot and subsequent root formation) conversion of PLBs to plantlets, Huang and Chung (2011) have also used a similar strategy for shoot (cytokinins BA and TDZ combination) and root (auxins IBA or NAA) formation of PLBs of Lycaste hybrid. A similar method of step wise conversion of PLBs was also reported earlier by Hossain et al (2010) in Cymbidium giganteum Wall.…”

Histología de la regeneración por organogénesis en Paulownia tomentosa (Paulowniaceae)

“…However, meristems can be formed indirectly from unspecialized cells in calluses or culture suspensions (Yancheva, Golubowicz, Fisher, Lev-Yadun, & Flaishman, 2003;Gahan & George, 2008). The direct and indirect origin of adventitious buds has been described for monocotyledons (e.g., Paphiopedilum spp., Chen, Chen, & Chang, 2004; Lycaste spp., Huang & Chung, 2011), dicotyledons (e.g., Citrus spp., Almeida et al, 2003; Malus x domestica, Yancheva et al, 2003;Passiflora edulis, Fernando et al, 2007;Centaurea ultreiae, Mallón, Rodríguez-Oubiña, & González, 2011) and conifers (e.g., Pinus massoniana, Zhang, Wei, Xi, & Shi, 2006;P. pinea, Cuesta, Rodríguez, Centeno, Ordas, & Fernán-dez, 2009).…”

Microtuberization and Acclimatization in the Dioscorea cayenensis Thunb. by the Carbon Source

“…(Protocorm Like Body) 및 뿌리줄기(rhizome)등을 이용한 조직 배양 방법은 일반적인 번식방법이 어려운 식물체에서 사용 되어왔다 (Ng and Saleh 2011;Huang and Chung 2011;Rayirath et al 2011;Torres-Morán et al 2010). 이러한 기내 비대경 생산을 통한 번식방법은 구황작물에서 가장 큰 문제가 되고 있는 바 이러스 및 병에 대한 저항성을 가진 무병주(virus or diseasefree plantlets)의 대량생산을 가능하게 하며, 작물의 특성상 장기저장과 이동이 어려운 유전자원의 저장 및 이동을 보다 용이하게 하였다 (Uchendu et al 2016).…”