Efficient inactivation of harmful algae K. mikimotoi by a novel algicidal bacterium via a rare direct contact pathway: Performances and mechanisms

Shi, Jialin; Wang, Wanjun; Wang, Fan; Lei, S.P.; Shao, Shaobin; Wang, Chao; Li, Guiying; An, Taicheng

doi:10.1016/j.scitotenv.2023.164401

Cited by 8 publications

(1 citation statement)

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“…The activities of SOD and CAT in the HAB species Phaeocystis globosa and Heterocurvium akashiwo were stimulated at lower concentrations of toxins but were inhibited at higher levels [28,29]. The malondialdehyde (MDA) level reflects the cell membrane lipid peroxidation degree [30] and is frequently utilized as an indicator for the extent of algal cell damage [31,32]. At the photosynthetic level, tracking chlorophyll fluorescence parameters indirectly indicates changes in photosynthesis [33].…”

Section: Introductionmentioning

confidence: 99%

Pilot-Scale Fermentation of Pseudoalteromonas sp. Strain FDHY-MZ2: An Effective Strategy for Increasing Algicidal Activity

Zhong,

Zheng,

Shi

et al. 2023

Biology

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The role of microorganisms in effectively terminating harmful algal blooms (HABs) is crucial for maintaining environmental stability. Recent studies have placed increased emphasis on bio-agents capable of inhibiting HABs. The bacterium Pseudoalteromonas sp. strain FDHY-MZ2 has exhibited impressive algicidal abilities against Karenia mikimotoi, a notorious global HAB-forming species. To augment this capability, cultures were progressively scaled from shake flask conditions to small-scale (5 L) and pilot-scale (50 L) fermentation. By employing a specifically tailored culture medium (2216E basal medium with 1.5% soluble starch and 0.5% peptone), under precise conditions (66 h, 20 °C, 450 rpm, 30 L/min ventilation, 3% seeding, and constant starch flow), a notable increase in algicidal bacterial biomass was observed; the bacterial dosage required to entirely wipe out K. mikimotoi within a day decreased from 1% to 0.025%. Compared to an unoptimized shake flask group, the optimized fermentation culture caused significant reductions in algal chlorophyll and protein levels (21.85% and 78.3%, respectively). Co-culturing induced increases in algal malondialdehyde and H2O2 by 5.98 and 5.38 times, respectively, leading to further disruption of algal photosynthesis. This study underscores the unexplored potential of systematically utilized microbial agents in mitigating HABs, providing a pathway for their wider application.

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Section: Introductionmentioning

confidence: 99%