Efficient in vitro labeling rabbit neural stem cell with paramagnetic Gd-DTPA and fluorescent substance

Shen, Jun; Cheng, Li-Na; Zhong, Xiaomei; Duan, Xiaohui; Guo, Ruomi; Hong, Guo-bing

doi:10.1016/j.ejrad.2009.04.040

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…As a consequence, ex vivo measurements of tissue samples could be performed after MRI studies. Such experiments could be highly relevant for discriminating hypointense areas owing to artifacts (tissue interfaces, presence of air) and hypointense regions owing to the labeled cells . Therefore, EPR could provide an important contribution to an unambiguous assessment of iron oxide content in tissues in MRI cell‐tracking experiments.…”

Section: Discussionmentioning

confidence: 99%

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Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies

Danhier

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Boutry

et al. 2012

Contrast Media & Molecular

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Introduction The ability to track the migration of metastatic cells when leaving the seeding tumor should become an invaluable tool to understand this phenomenon and propose new therapeutic anti-cancer strategies. Magnetic cell labeling is a promising technique to detect metastatic cancer cells with magnetic resonance imaging. Usually, cells are incubated with iron oxides (T 2 contrast agent) in order to uptake the particles before being injected in vivo. In addition to MRI protocols, there is generally a need for complementary techniques able to confirm and quantify the cells that have migrated inside a host tissue. We propose here to implement Electron Paramagnetic Resonance (EPR) as a very sensitive method to quantify iron oxide concentration (in cells and tissues). Iron oxide particles exhibit an EPR spectrum, which directly reflects the number of iron oxide particles in a sample. EPR spectroscopy has already been proposed as a method of quantifying the accumulation of iron oxide inside tissues (1). In order to compare EPR with existing methods (Perl's Prussian blue reaction, and fluorimetry), we labeled tumor cells (Melanoma B16F10-luc, fibrosarcoma KHT-luc) and fibroblasts (3T3) with fluorescent iron oxide particles, and defined the limit of detection of the different techniques.

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Section: Discussionmentioning

confidence: 99%

“…the labeled cells(13,21). Therefore, EPR could provide an important contribution to an unambiguous assessment of iron oxide content in tissues in MRI celltracking experiments.…”

mentioning

confidence: 99%

Electron paramagnetic resonance as a sensitive tool to assess the iron oxide content in cells for MRI cell labeling studies

Danhier

Preter

Boutry

et al. 2012

Contrast Media & Molecular

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“…Lipofection was reported to enable efficient labeling of ES cells and neural progenitor cells with the superparamagnetic contrast agent Sinerem (Kustermann et al, 2008). The single reports on lipofection of stem cells with paramagnetic contrast agents made use of Lipofectin and Lipofectamin (Rudelius et al, 2003), or Effectene (Shen et al, 2009). Recently, Guenoun et al (2011) described a cationic Gd-DTPA liposome complex for enhanced labeling efficiency of mesenchymal stem cells.…”

Section: Cell Labeling Efficiencymentioning

confidence: 97%

In vivo imaging of inhibitory, GABAergic neurons by MRI

et al. 2012

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The unambiguous detection of specific neuronal subtypes is up to now only possible with invasive techniques or optical imaging after genetic modification. High field magnetic resonance imaging (MRI) has the ability to visualize the brain structure and anatomy noninvasively, with high resolution--but missing the cell specific and functional information. Here we present a new tool for neuroimaging with MRI, enabling the selective detection of GABAergic neurons under in vivo conditions. The specific imaging contrast is achieved by a novel paramagnetic contrast agent, which responds to the activity of the enzyme glutamic acid decarboxylase--expressed solely by inhibitory neurons. The relaxivity of the complex is increased upon decarboxylation of two glutamic acid moieties, thus allowing increased water access to the inner and outer coordination spheres of the paramagnetic ion. The mechanism and specificity of activation were proven with tissue lysates and further applied to a differentiation protocol for murine embryonic stem cells. The relaxation enhancement was studied quantitatively and revealed decreased longitudinal relaxation times in the inhibitory neuron samples compared to the naïve stem cells in vitro and in vivo. Furthermore, this approach offers not only the discrimination of inhibitory, GABAergic neurons in the brain but also may expand the usefulness of MRI for functional imaging on a cellular level.

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“…For further facilitate the therapeutic effects using BMSCs, cell imaging plays a pivotal role in this regenerative therapy study [31]. When BMSCs were labled by Gadolinium-diethylene triamine pentaacetic acid (Gd-DTPA), the migration and proliferation of BMSCs could be traced by MRI in animal models.…”

Section: Editorialmentioning

confidence: 99%