Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.

Bártek, Jiří; Bártková, Jiřina; Kyprianou, Natasha; Lalani, El–Nasir; Stasková, Z; Shearer, Moira; Chang, Sidney E.; Taylor‐Papadimitriou, Joyce

doi:10.1073/pnas.88.9.3520

Cited by 147 publications

(121 citation statements)

References 26 publications

(11 reference statements)

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“…Mycoplasma-free HB2 cells [a subclone of the MTSVI-7 line (Bartek et al, 1991)] obtained from Dr Joyce Taylor-Papadimitriou (Imperial Cancer Research Fund, Lincoln's Inn Fields, UK) were cultured as described previously . Briefly, cells were seeded at different densities into a 10-cm Beckton-Dickinson tissue culture plate in Dulbecco's modified Eagle medium (DMEM) (Clare Hall Laboratories, Imperial Cancer Research Fund, UK) supplemented with 10% fetal calf serum (FCS), 10 g ml bovine insulin and 5 jg ml-' hydrocortisone in 95% air, 5% carbon dioxide, at 37°C.…”

Section: Materials and Methods Cell Culturementioning

confidence: 99%

Regulation of Wnt5a expression in human mammary cells by protein kinase C activity and the cytoskeleton

Jönsson

Smith²,

Harris³

1998

Br J Cancer

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Section: Materials and Methods Cell Culturementioning

confidence: 99%

Regulation of Wnt5a expression in human mammary cells by protein kinase C activity and the cytoskeleton

Jönsson

Smith²,

Harris³

1998

Br J Cancer

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“…The human mammary epithelial cell line 184A1L5 (37) was obtained from Dr. Martha Stampfer and was cultured in medium DFCI-1 as described (38). The human mammary epithelial line HB2 was a gift from Joyce Taylor-Papadimitriou and were grown as described (39). SKBR3 cells (40) were obtained from the American Type Culture Collection and grown in McCoys 5A supplemented with 10% fetal calf serum (HyClone).…”

Section: Methodsmentioning

confidence: 99%

Structural Aspects of the Epidermal Growth Factor Receptor Required for Transmodulation of erbB-2/neu

Worthylake

Wiley

1997

Journal of Biological Chemistry

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The epidermal growth factor receptor (EGF-R) is known to transmodulate the activity and level of the erbB-2/neu protein in several epithelial cell lines. We therefore determined which structural features of the EGF-R were important in transmodulating erbB-2. We found that the addition of EGF to nontransformed epithelial cells resulted in down-regulation of erbB-2 with the same kinetics and similar extent as the EGF-R. By using cells expressing a series of EGF-R modified by site-directed mutagenesis, we found that EGF-R tyrosine kinase activity was not necessary for down-regulation of erbB-2, but receptor sequences between 899 and 958 in the EGF-R were required. To determine whether transmodulation was associated with activation of erbB-2, tyrosine phosphorylation of erbB-2 was determined following addition of EGF. Again, phosphorylation of erbB-2 following EGF addition did not require the intrinsic tyrosine kinase activity of the EGF-R, but did require sequences between 899 and 958. To determine the localization of EGF-R and erbB-2 following EGF addition, the relative distribution of the two receptors was evaluated by fluorescence microscopy. Surprisingly, the majority of erbB-2 was found in small cytoplasmic vesicles, whereas the EGF-R was predominantly found on the cell surface. Addition of EGF resulted in a redistribution and consequent colocalization of both receptors in endosomal and lysosomal structures. We conclude that activation and transmodulation of erbB-2 does not require intrinsic tyrosine kinase activity of the EGF-R, but does require sequences in the EGF-R which regulate its trafficking.

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“…The first established nonmalignant breast epithelial cell line with a profile similar to human breast cancer was generated by immortalization with SV-40 (Bartek et al 1991). Apparently, human breast cells have different thresholds for immortalization, and this is undoubtedly lineage dependent (Romanov et al 2001;Rangarajan et al 2004).…”

Section: Established Cell Linesmentioning

confidence: 99%

Stem Cells in the Human Breast

Petersen¹,

Polyák²

2010

Cold Spring Harbor Perspectives in Biology

100

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Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus.

Abstract: When defined in terms of markers for normal cell lineages, most invasive breast cancer cells correspond to the

Cited by 147 publications

References 26 publications

Regulation of Wnt5a expression in human mammary cells by protein kinase C activity and the cytoskeleton

Regulation of Wnt5a expression in human mammary cells by protein kinase C activity and the cytoskeleton

Structural Aspects of the Epidermal Growth Factor Receptor Required for Transmodulation of erbB-2/neu

Stem Cells in the Human Breast

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