Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

Abstract

Abstract-Gold screen-printed electrodes coated with thin films of layered double hydroxides containing cobalt and aluminium (Co 1.57 Al(OH) x SO 4 , shortened as CoAl) have been investigated for the design of an electrochemical tyrosinasebased biosensor used for the detection of a complex mixture of polyphenols extracted from green tea. Physicochemical analyses show that the resulted biosensor exhibits very attractive characteristics: a high sensitivity, a large dynamic range (up to 1000 ng.mL −1 ), and very low limits of detection (0.33 pg.mL −1 and 0.03 pg.mL −1 for oxidation and reduction, respectively).Index Terms-Atomic force microscopy, electrochemical biosensor, layered double hydroxide, polyphenols, tyrosinase.

Electrochemical Detections of Tea Polyphenols: A Review

“…As an extension, the biosensor was used in the detection of polyphenols in tea and wine samples and was compared with the conventional technique (HPLC). In 2017, Soussou et al., reported the determination of tea polyphenols, using Ty enzyme, immobilized over the layered double hydroxide (LDH) hybrid of cobalt and aluminum (designated as CoAl) modified gold SPE (AuSPE) [49]. The CME was designated as AuSPE/CoAl/Ty.…”

Layered Double Hydroxides Nanomaterials in Biomedicine and (bio) Sensing Design

“…Layered Double Hydroxide in (bio) Sensing: Layered double hydroxides are nanomaterials with high stability, low toxicity, variable morphological properties and significant adsorption capacity. Due to these characteristics, LDHs were perfect candidates for the immobilization of different molecules and biomolecules as DNA, drugs and enzymes [42]. Furthermore, they can be considerate as promising materials for electrochemical sensors and biosensor design.…”

“…The enzymes are biomolecules with catalytic activity which can be used in different processes due to their high specificity and efficiency [43]. LDHs nanocomposites have proven to be suitable supports for enzyme immobilization, without notable modification of enzyme activity [42]. One of the most important directions of enzyme immobilization on LDHs nanocomposites is their utilization in biosensing.…”

“…A robust polyphenols biosensor was designed by covering gold electrodes with LDH, followed by tyrosinase binding to the modified electrodes. The biosensor was used many times with good performances, and it is appropriate to be used in fields where traces need to be detected (medicine, environment) [42]. Horseradish peroxidase (HRP) is an oxidoreductase used in biosensig for H2O2 detection.…”

Immobilization and Performance of Cellulase on Recyclable Magnetic Hydrotalcites

“…vn by co-precipitation in the presence of Fe3O4 produces magnetic and porous support for enzyme immobilization. Several enzymes were immobilized on HT and used for biosensor application such as dextranase (Ding et al, 2018), peroxidase Baccar & Hafaiedh, 2011;Hidouri et al, 2021), superoxide−dismutase (Szilágyi et al, 2018), laccase (Camacho Córdova et al, 2009), lactate−dehydrogenase (Djebbi et al, 2016), tyrosinase (Soussou et al, 2017). Starch hydrolytic enzymes such as amylase (Bruna et al, 2015;Sahutoglu & Akgul, 2015), lipase (Dias et al, 2019), glucosidase, and cellulase (Zhang et al, 2020) are attracted by research groups.…”