Efficient Immobilization of a Cadmium Chemosensor in a Thin Film:  Generation of a Cadmium Sensor Prototype

Bronson, R. Todd; Michaelis, David J.; Lamb, Randy D.; Husseini, Ghaleb A.; Farnsworth, Paul B.; Linford, Matthew R.; Izatt, Reed M.; Bradshaw, Jerald S.; Savage, Paul B.

doi:10.1021/ol050027t

Cited by 124 publications

(40 citation statements)

References 14 publications

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“…45 nM). Although the LOD for Cd 2+ in this study does not meet the requirement by EPA, it is comparable to that of a thin film Cd 2+ chemosensor (1.0 M) [49] and much lower than that of laser induced breakdown spectroscopy technique (500 mg L −1 , i.e. 4.5 × 10 −3 M) [50].…”

Section: Detection Of Zn 2+ and CD 2+ Based On S 2− Modified Cdte Qdscontrasting

confidence: 62%

Quantum dot-based “turn-on” fluorescent probe for detection of zinc and cadmium ions in aqueous media

Hu¹,

Miao²,

Fang³

et al. 2011

Analytica Chimica Acta

144

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Section: Detection Of Zn 2+ and CD 2+ Based On S 2− Modified Cdte Qdscontrasting

confidence: 62%

Quantum dot-based “turn-on” fluorescent probe for detection of zinc and cadmium ions in aqueous media

Hu¹,

Miao²,

Fang³

et al. 2011

Analytica Chimica Acta

144

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“…Methods for immobilizing proteins on gold film, which have been investigated for years, include the SPA method (15,23 ), the avidin and biotin method (24,25 ), and the self-assembled monolayers method (26,27 ). The SPA method was adopted in the present experiment because it can create more reproducible and stable immobilization layers with less added mass for IgG coating (28 ).…”

Section: Results and Discussion Optimization Of Immobilized Antibody mentioning

confidence: 99%

Rapid and Simultaneous Quantification of 4 Urinary Proteins by Piezoelectric Quartz Crystal Microbalance Immunosensor Array

Luo¹,

Chen²,

Qin

et al. 2006

Clinical Chemistry

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Background: Urinary proteins are predictive and prognostic markers for diabetes nephropathy. Conventional methods for the quantification of urinary proteins, however, are time-consuming, and most require radioactive labeling. We designed a label-free piezoelectric quartz crystal microbalance (QCM) immunosensor array to simultaneously quantify 4 urinary proteins. Methods: We constructed a 2 ؋ 5 model piezoelectric immunosensor array fabricated with disposable quartz crystals for quantification of microalbumin, ␣ 1 -microglobulin, ␤ 2 -microglobulin, and IgG in urine. We made calibration curves after immobilization of antibodies at an optimal concentration and then evaluated the performance characteristics of the immunosensor with a series of tests. In addition, we measured 124 urine samples with both QCM immunosensor array and immunonephelometry to assess the correlation between the 2 methods. Results: With the QCM immunosensor array, we were able to quantify 4 urinary proteins within 15 min. This method had an analytical interval of 0.01-60 mg/L. The intraassay and interassay imprecisions (CVs) were <10%, and the relative recovery rates were 90.3%-109.1%. Nonspecificity of the immunosensor was insignificant (frequency shifts <20 Hz). ROC analyses indicated sensitivities were >95.8% and, specificities were

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“…For quenching of FL intensity in the presence of Hg 2+ ion, the reason maybe attribute to the effect between heavy metal ions such as Hg 2+ (4f 14 5d 10 ), Pb 2+ (4f 14 6d 10 ) and Au + (4f 14 5d 10 ) [18,[27][28][29][30]. That is, BSA macromolecules, which contains lots of amino or carbonyl C=O group/ s in the skeleton/surface [31], and Cd 2+ ion has much stronger affinity for nitrogen/oxygen atoms of amino or carbonyl C=O group/s ( Figure S7) [32][33][34][35]. And assuming amino or carbonyl C=O group/s produce a moderate quenching to the FL BSA-Au-Ni NCs by PET.…”

Section: Optimization Of Methodsmentioning

confidence: 99%

Fluorometric determination of cadmium(II) and mercury(II) using nanoclusters consisting of a gold-nickel alloy

2015

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A one-pot route has been developed for the preparation of bovine serum albumin-templated nickel-doped bimetallic gold-nickel nanoclusters (BSA-Au-Ni NCs) at a 10:1 M ratio of the precursor salts in a BSA matrix under alkaline conditions. The metal ions are reduced to the metal alloys by BSA. The resulting NCs display strong fluorescence and dual emission with peaks at 405 and 640 nm, respectively, under excitation at 340 nm. Fluorescence is strongly enhanced on addition of Cd(II) ions, but quenched on addition of Hg(II) ions. The findings have been exploited to design a fluorometric method for the separate determination of Cd(II) and Hg(II), respectively. The optimized analytical nanosystem displays relatively good dynamics between enhancement and quenching. Cd(II) and Hg(II) can be quantified in the 0 to 200 and 0 nM to 24 μM, respectively. The limits of detection are~1.8 nM in both cases, which indicates the highest sensitivity to Cd(II) and Hg(II) ions for a fluorescent probe. This new kind of nanocrystal probe is hardly interfered by a range of commonly encountered metal ions. Its advantages were demonstrated by determining Cd(II) and Hg(II) ions in spiked serum samples.

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Efficient Immobilization of a Cadmium Chemosensor in a Thin Film: Generation of a Cadmium Sensor Prototype

Cited by 124 publications

References 14 publications

Quantum dot-based “turn-on” fluorescent probe for detection of zinc and cadmium ions in aqueous media

Quantum dot-based “turn-on” fluorescent probe for detection of zinc and cadmium ions in aqueous media

Rapid and Simultaneous Quantification of 4 Urinary Proteins by Piezoelectric Quartz Crystal Microbalance Immunosensor Array

Fluorometric determination of cadmium(II) and mercury(II) using nanoclusters consisting of a gold-nickel alloy

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