EfficientIn VitroRegeneration of Sugarcane (Saccharum OfficinarumL.) from Bud Explants

Zamir, Roshan; Khalil, Shahid Akbar; Shah, Syed Tariq; Khan, Muhammad Shuaib; Ahmad, Kafeel; Shahenshah,; Ahmad, Nisar

doi:10.5504/bbeq.2012.0049

Cited by 8 publications

(4 citation statements)

References 35 publications

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“…With respect to root induction, 25% of shoot clusters of LSC formed well-defined roots spontaneously on BAPcontaining medium (SPM) after 4 weeks of culture compared to 57% in B36464 shoot clusters (Figure 4C). This phenomenon of spontaneous root formation at the shoot maturation stage on media containing only cytokinins has been reported by Zamir et al (2012). Nonetheless, to improve root formation from in vitro shoots, we supplemented MS media with 3 mg/L NAA which resulted in marked improvement in root development in both B36464 (88%) and LSC (72%) after 8 weeks of culture.…”

Section: Shoot Proliferation and Root Inductionsupporting

confidence: 54%

Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana

Azu¹,

Elegba²,

Asare³

et al. 2022

Afr. J. Biotechnol.

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An efficient callus-mediated regeneration system was developed for high-frequency production of planting material of sugarcane genotypes LSC and B36464. Spindle leaf segments cultured on MS basal medium supplemented with 2,4-D or picloram at 1, 2, 3 or 4 mg/L resulted in callus induction. Callus induction was higher on 2,4-D amended medium compared to picloram. Nevertheless, for both auxins, callus induction improved significantly (p ≤ 0.05) with increasing concentration; the highest (82 and 82.5% for B36464 and LSC respectively) was achieved at 4 mg/L. For shoot induction, calli were transferred to MS medium supplemented with BAP (0.1, 0.5, 1.0 or 1.5 mg/L). The highest number of shoots (18.13 and 16.75 for B36464 and LSC respectively) was achieved at 1.5 mg/L. Serial subculture at four-week intervals on a higher concentration of BAP (2.5 mg/L), in combination with NAA (0.5 mg/L) and GA 3 (0.5 mg/L), resulted in a four-fold increase in shoot number within 16 weeks. On this medium, 40% of shoot clusters of B36464 formed well-defined shoots. On MS medium containing solely NAA (3 mg/L), 88 and 72% (B36464 and LSC respectively) formed roots. Post-flask acclimatisation of the plantlets led to 85 and 91% survival rates in LSC and B36464 respectively after which plantlets were successfully transferred to field conditions. The callus-mediated regeneration system reported in this study has the potential to sustainably provide sugarcane planting material for the emerging sugar industry in Ghana.

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Section: Shoot Proliferation and Root Inductionsupporting

confidence: 54%

Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana

Azu¹,

Elegba²,

Asare³

et al. 2022

Afr. J. Biotechnol.

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“…In contrast, somatic embryos were reported to be induced at lower concentrations of 2,4-D (1 mg/l), whereas higher concentrations induced non-embryogenic calli (Zamir et al, 2014). Sequential removal of 2,4-D followed by sub-culture on MS supplemented with 2mg/l BAP induced 98% shoot induction (after 3 weeks of culture) with maximum shoot elongation (9.4 cm) (Zamir et al, 2014). Similarly, callus induced in MS + 3.0 mg/l 2,4-D and subsequently incubated on 2,4-D-free media was found to be commercially viable for plantlet regeneration on MS + 1.0-1.5 mg/l BAP + 0.2 mg/l NAA in elite sugarcane genotypes (Abdu et al, 2012).…”

Section: Response To In Vitro Culturementioning

confidence: 92%

“…Khamrit et al (2012) reported maximum percentage of somatic embryogenic callus induction in MS medium supplemented with 3 mg/l 2,4-D and 15% (v/v) coconut water. In contrast, somatic embryos were reported to be induced at lower concentrations of 2,4-D (1 mg/l), whereas higher concentrations induced non-embryogenic calli (Zamir et al, 2014). Sequential removal of 2,4-D followed by sub-culture on MS supplemented with 2mg/l BAP induced 98% shoot induction (after 3 weeks of culture) with maximum shoot elongation (9.4 cm) (Zamir et al, 2014).…”

Section: Response To In Vitro Culturementioning

confidence: 99%

Cyanosiphovirus S-ESS1 Infecting Marine Synechococcus (Chroococcales) Almost Shows No Genetic Relationship to Known Cyanosiphoviruses

Han¹,

Zhang²,

Zhao³

et al. 2017

gab

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Sugarcane is a cash crop of national importance. Its complex genome, narrow gene pool, long breeding cycle, rare flowering and complex environmental interactions hinders progress in genetic improvement. But, the crop serves as an excellent material for in vitro culture. Therefore, genetic transformation can be a better alternative to incorporate resistance to diseases and abiotic stresses, and genetic improvement of quality traits. In this pursuit, the authors presented a detailed review of the status of in vitro culture and current strategies of genetic transformation in sugarcane using a number of important candidate genes.

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“…In Pakistan, this pathogen first reported in 1986 by Ahmed et al, 1986. The major reason of common of red rot disease in Pakistan is due to cultivation of susceptible Sugarcane varieties that difficult to incorporate the new sugarcane cultivars with developed agronomic features and resistance to biotic and abiotic stresses (Zamir et al, 2012). Red rot of sugarcane may infect developed stems of cane, leaf mid ribs which results in considerable damages in sugar quality (Rao et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Screening of Sugarcane Cultivars Against Colletotrichum Falcatum Causing Red Rot Disease and Its Control With Different Fungicides Under Laboratory Conditions

Ghazanfar

Raza

Gondal

2017

Pak. J. Phytopathol.

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A B S T R A C TTen sugarcane (Saccharum officinarum L.) cultivars were screened against red rot disease caused by Colletotrichum falcatum under laboratory conditions by artificial inoculation technique. These cultivars were graded under various levels of resistance as well as susceptibility using a standard disease rating scale. Two cultivars i.e. NSG-59 and SPF-244 showed resistant reaction to red rot of sugarcane. Three cultivars i.e. CPF-246, CPF-247 and BF-138 showed moderately resistant (MR) reaction against red rot. Remaining five cultivars showed moderate, susceptible to highly susceptible reaction. The results of in vitro evaluation of seven fungicides at four concentrations (10 ppm, 15 ppm, 20 ppm and 25 ppm) showed that all tested fungicides with all concentrations significantly inhibited the mycelial growth of the pathogen as compared with control. However, the inhibition percentage was increased by increasing the concentrations of tested fungicides. Among fungicides Tilt proved the most effective fungicide by inhibiting linear mycelial growth at all concentrations against of C. falcatum followed Nativo while Metaxyl&Mencozeb was the least effective in terms of retarding fungal growth. The findings of the present study suggested that resistant cultivars may be utilized as a source of resistance and may be more useful as donors in breeding programme aimed at red rot disease resistance and the growth of the pathogen is effected by different concentrations of fungicides may play an important role to manage this disease.

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EfficientIn VitroRegeneration of Sugarcane (Saccharum OfficinarumL.) from Bud Explants

Cited by 8 publications

References 35 publications

Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana

Efficient callus-mediated system for commercial production of sugarcane (Saccharum spp.) planting material in Ghana

Cyanosiphovirus S-ESS1 Infecting Marine <i>Synechococcus</i> (<i>Chroococcales</i>) Almost Shows No Genetic Relationship to Known Cyanosiphoviruses

Screening of Sugarcane Cultivars Against Colletotrichum Falcatum Causing Red Rot Disease and Its Control With Different Fungicides Under Laboratory Conditions

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