Efficient heterologous expression of formate dehydrogenase and preliminary determination of the potential for conversion of carbon dioxide to formate

Yuan, S.L.; Fu, Meng-Xi; Xian, Yang Ting; Shi, Hongbo; Chunchang,; Yao, Lunguang; Tang, Cun-Duo; Chen, Chang-Po

doi:10.1016/j.mcat.2023.113455

Cited by 1 publication

(2 citation statements)

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“…Figure A shows formic acid production catalyzed by Pb FDH and Ss FDH with or without H 2 . Ss FDH is a NAD + -dependent FDH derived from Sclerotinia sclerotiorum, which demonstrates relatively high catalytic activity toward CO 2 reduction . Formic acid production catalyzed by Pb FDH and Ss FDH with H 2 yielded 19.3 and 12.6 mM after reaction for 48 h, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…To calculate the kinetic parameters for CO 2 , CO 2 and sodium bicarbonate were used as the substrates with 10 mM NADH. Because measuring the accurate concentration of CO 2 in the aqueous phase was difficult, Henry’s law was utilized to calculate CO 2 concentration in the aqueous phase C aqueous , normalC O 2 = H normalC O 2 × P normalC O 2 where C aqueous,CO 2 denotes the CO 2 concentration in the aqueous phase, H CO 2 denotes Henry’s constant for CO 2 (3.4 × 10 –2 mol L –1 atm –1 ), and P CO 2 denotes the partial pressure of CO 2 . Based on this equation, 29 and 74% of CO 2 balanced with N 2 correspond to 10 and 25 mM CO 2 in the aqueous phase, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Engineered Escherichia coli Whole Cell-Mediated Electro-Biocatalysis for Carbon Dioxide to Formic Acid Conversion

Shi,

Fu,

Yuan

et al. 2024

ACS Sustainable Chem. Eng.

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The conversion of CO 2 into a valuable chemical fuel could help reduce its effect on global warming. However, the utilization of CO 2 by biological transformations is challenging because of the lack of enzymes exhibiting high catalytic activity toward CO 2 reduction. In this work, three NAD + /W-containing formate dehydrogenases (FDHs) were discovered, expressed, and characterized. In addition, we used PbFDH, which displays high catalytic activity toward CO 2 reduction, as a biocatalyst to convert CO 2 to formic acid through whole-cell biocatalysis and electrobiocatalysis. The specific activities of DaFDH, PbFDH, and CsFDH increased by 68.1, 100.0, and 18.7 times, respectively, compared with that of ClFDH reported with high catalytic efficiency. Furthermore, this paper presents a preliminary discussion of the catalytic mechanism of FDHs for CO 2 reduction based on their structures. The yield of formic acid obtained from CO 2 reduction using electro-biocatalysis under aerobic conditions reaches up to 4.1 mmol/L/h, without any cofactor NADH and hydrogen gas. This study also demonstrates and compares the performances of NAD + /W-containing and NAD + -dependent FDHs in whole-cell biocatalysis and electro-biocatalysis. The findings of this study provide a meaningful foundation for the conversion of CO 2 into a value-added chemical fuel.

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Section: Resultsmentioning

confidence: 99%