Efficient genetic transformation method for Eucalyptus genome editing

Abstract

Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis genetic background, germplasm improvement by crossbreeding tends to be inefficient. As an alternative approach, genetic engineering of Eucalyptus can be used to effectively improve germplasm resources. From a strategic standpoint, increasing the plantation productivity and wood quality by transgenic technology has become increasingly important for forest industry. In this study, we established a fluorescence labelling method using CRISPR/Cas9 technology to obtain positive transformed progenies. The positive transformed progenies were easily obtained from the genetically modified population via fluorescence screening. This system can be used as a plant genome site-specific editing tool and may be useful for improving Eucalyptus genetic resources.

Efficiency evaluation and nonlinear multi-objective optimization of forestry industry transformation in the Heilongjiang state-owned forest region

“…Scientific and technological innovation in the forestry industry should be accelerated, giving play to the government's public service function and promoting the transformation and upgrading of the forestry industry 12 . Wang et al 13 pointed out that industrial transformation and upgrading were the basic means to transform the mode of economic growth, improve economic development efficiency, and achieve rapid and sound economic development. Only through industrial transformation can people completely solve the problems faced by resource-based industries.…”

“…The industrial transformation was the only way for the state-owned forest industry to develop to a higher level. The healthy and stable development of the forestry industry was a crucial guarantee for promoting forestry production efficiency, the living conditions of forest residents, the income of forestry workers, and the coordinated development of the economy, society, resources, and environment 13 . To sum up, the current forest resources had been destroyed, so optimizing forest resource protection measures was one of the crucial tasks in social development.…”