Efficient generation of chimaeric mice using embryonic stem cells after long-term culture in the presence of ciliary neurotrophic factor

Wolf, Eckhard; Kramer, Rainer; Polejaeva, Irina A.; Thoenen, H.; Brem, Gottfried

doi:10.1007/bf01973982

Cited by 34 publications

(17 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The factors that fibroblasts produce that prevent the differentiation of R278.5 cells or feeder-dependent human EC cells are unknown. Other factors that fail to support the growth of feeder-dependent human EC cells in the absence of feeder layers include oncostatin M and ciliary neurotrophic factor (19), both of which can substitute for LIF in preventing the differentiation of mouse ES cells (32,33). A trypsin-sensitive factor from a human yolk sac carcinoma cell line (GCT 44) supports the growth of feederdependent human EC cells in the absence of fibroblasts, but the factor has not yet been purified (19).…”

Section: Discussionmentioning

confidence: 99%

Isolation of a primate embryonic stem cell line.

Thomson

Kalishman

Golos

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

983

609

View full text Add to dashboard Cite

Embryonic stem cells have the ability to remain undifferentiated and proliferate indefinitely in vitro while maintaining the potential to differentiate into derivatives of all three embryonic germ layers. Here we report the derivation of a cloned cell line (R278.5) from a rhesus monkey blastocyst that remains undifferentiated in continuous passage for >1 year, maintains a normal XY karyotype, and expresses the cell surface markers (alkaline phosphatase, stage-specific embryonic antigen 3, stage-specific embryonic antigen 4, TRA-1-60, and TRA-1-81) that are characteristic of human embryonal carcinoma cells. R278.5 cells remain undifferentiated when grown on mouse embryonic fibroblast feeder layers but differentiate or die in the absence of fibroblasts, despite the presence of recombinant human leukemia inhibitory factor. R278.5 cells allowed to differentiate in vitro secrete bioactive chorionic gonadotropin into the medium, express chorionic gonadotropin a-and ,8-subunit mRNAs, and express a-fetoprotein mRNA, indicating trophoblast and endoderm differentiation. When injected into severe combined immunodeficient mice, R278.5 cells consistently differentiate into derivatives of all three embryonic germ layers. These results define R278.5 cells as an embryonic stem cell line, to our knowledge, the first to be derived from any primate species.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation of a primate embryonic stem cell line.

Thomson

Kalishman

Golos

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

983

609

View full text Add to dashboard Cite

show abstract

“…We tested whether this was a general property of signalling through GP130, as other members of the same cytokine family, such as IL-6, have also been associated with self-renewal (Conover et al, 1993;Rose et al, 1994;Wolf et al, 1994;Yoshida et al, 1994;Pennica et al, 1995). Fig.…”

Section: Lif Promotes An Extraembryonic Esc Population Through the Jamentioning

confidence: 99%

LIF supports primitive endoderm expansion during pre-implantation development

Morgani

Brickman

2015

Development

View full text Add to dashboard Cite

Embryonic stem cells (ESCs) are pluripotent cell lines that can be maintained indefinitely in an early developmental state. ESC culture conditions almost always require the cytokine LIF to maintain selfrenewal. As ESCs are not homogeneous but contain multiple populations reminiscent of the blastocyst, identifying the target cells of LIF is necessary to understand the propagation of pluripotency. We recently found that LIF acts under self-renewing conditions to stimulate the fraction of ESCs that express extraembryonic markers, but has little impact on pluripotent gene expression. Here, we report that LIF has two distinct roles: it blocks early epiblast (Epi) differentiation, and it supports the expansion of primitive endoderm (PrE)-primed ESCs and PrE in vivo. We find that activation of JAK/ STAT signalling downstream of LIF occurs initially throughout the preimplantation embryo, but later marks the PrE. Moreover, the addition of LIF to cultured embryos increases the GATA6 + PrE population, whereas inhibition of JAK/STAT signalling reduces both NANOG + epiblast and GATA6 + PrE. The reduction of the NANOG + Epi might be explained by its precocious differentiation to later Epi derivatives, whereas the increase in PrE is mediated both by an increase in proliferation and inhibition of PrE apoptosis that is normally triggered in embryos with an excess of GATA6 + cells. Thus, it appears that the relative size of the PrE is determined by the number of LIF-producing cells in the embryo. This suggests a mechanism by which the embryo adjusts the relative ratio of the primary lineages in response to experimental manipulation.

show abstract

“…CNTF can promote self-renewal or maintenance of neural precursors in vitro (Chojnacki et al, 2003;Hitoshi et al, 2004) and maintain embryonic stem cell pluripotency in vitro (Wolf et al, 1994). Astrocytes, which produce CNTF, promote prolifera- tion and neuronal specification of hippocampal precursors in vitro (Song et al, 2002).…”

Section: Midbrain Dopaminergic Projections Enhance Normal Patterns Ofmentioning

confidence: 99%

Ciliary Neurotrophic Factor Mediates Dopamine D₂Receptor-Induced CNS Neurogenesis in Adult Mice

Yang¹,

Arnold²,

Habas³

et al. 2008

J. Neurosci.

139

149

View full text Add to dashboard Cite

Neurogenesis continues in the adult forebrain subventricular zone (SVZ) and the dentate gyrus of the hippocampal formation. Degeneration of dopaminergic projections in Parkinson's disease and animals reduces, whereas ciliary neurotrophic factor (CNTF) promotes, neurogenesis. We tested whether the dopaminergic system promotes neurogenesis through CNTF. Astrocytes of the SVZ and dentate gyrus expressed CNTF and were close to dopaminergic terminals. Dopaminergic denervation in adult mice reduced CNTF mRNA by ϳ60%, whereas systemic treatment with the D 2 agonist quinpirole increased CNTF mRNA in the SVZ and hippocampal formation, and in cultured astrocytes by 1.5-5 fold. The effect of quinpirole in vitro was blocked by the D 2 antagonist eticlopride and did not cause astroglial proliferation or hypertrophy. Systemic quinpirole injections increased proliferation in wild-type mice by ϳ25-75% but not in CNTF

show abstract

Efficient generation of chimaeric mice using embryonic stem cells after long-term culture in the presence of ciliary neurotrophic factor

Cited by 34 publications

References 34 publications

Isolation of a primate embryonic stem cell line.

Isolation of a primate embryonic stem cell line.

LIF supports primitive endoderm expansion during pre-implantation development

Ciliary Neurotrophic Factor Mediates Dopamine D₂Receptor-Induced CNS Neurogenesis in Adult Mice

Contact Info

Product

Resources

About

Efficient generation of chimaeric mice using embryonic stem cells after long-term culture in the presence of ciliary neurotrophic factor

Cited by 34 publications

References 34 publications

Isolation of a primate embryonic stem cell line.

Isolation of a primate embryonic stem cell line.

LIF supports primitive endoderm expansion during pre-implantation development

Ciliary Neurotrophic Factor Mediates Dopamine D2Receptor-Induced CNS Neurogenesis in Adult Mice

Contact Info

Product

Resources

About

Ciliary Neurotrophic Factor Mediates Dopamine D₂Receptor-Induced CNS Neurogenesis in Adult Mice