Efficient Gamma‐Retroviral Transduction of Primary Human Skin Cells Using the EF‐c Peptide as a Transduction Enhancer

Barbier, Martin A.; Piaceski, Angela Dakiw; Larouche, Danielle; Villeneuve, Sarah H; Ghani, Karim; Pope, Elena; Caruso, Manuel; Germain, Lucie

doi:10.1002/cpz1.353

Cited by 1 publication

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References 32 publications

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“…For example, several positively charged polycationic molecules, such as polybrene, which has been widely used in many clinical studies, have applied to increase in vivo retroviral gene delivery efficiency. Recently, Barbier et al (2022) developed a system for the efficient therapeutic gene transduction to keratinocytes and dermal fibroblasts in vivo using EF-c, that forms amyloid-like peptide nanofibrils. Similarly, Dakiw Piaceski et al (2018) also combined therapeutic gene (COL7A1 gene, encoding type VII collagen) and tissue-engineered skin substitute for treating dystrophic EB.…”

Section: Regeneration and Repair Of Skin Woundsmentioning

confidence: 99%

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

Hong

2022

Front. Cell Dev. Biol.

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Stem cell-based therapeutics have gained tremendous attention in recent years due to their wide range of applications in various degenerative diseases, injuries, and other health-related conditions. Therapeutically effective bone marrow stem cells, cord blood- or adipose tissue-derived mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and more recently, induced pluripotent stem cells (iPSCs) have been widely reported in many preclinical and clinical studies with some promising results. However, these stem cell-only transplantation strategies are hindered by the harsh microenvironment, limited cell viability, and poor retention of transplanted cells at the sites of injury. In fact, a number of studies have reported that less than 5% of the transplanted cells are retained at the site of injury on the first day after transplantation, suggesting extremely low (<1%) viability of transplanted cells. In this context, 3D porous or fibrous national polymers (collagen, fibrin, hyaluronic acid, and chitosan)-based scaffold with appropriate mechanical features and biocompatibility can be used to overcome various limitations of stem cell-only transplantation by supporting their adhesion, survival, proliferation, and differentiation as well as providing elegant 3-dimensional (3D) tissue microenvironment. Therefore, stem cell-based tissue engineering using natural or synthetic biomimetics provides novel clinical and therapeutic opportunities for a number of degenerative diseases or tissue injury. Here, we summarized recent studies involving various types of stem cell-based tissue-engineering strategies for different degenerative diseases. We also reviewed recent studies for preclinical and clinical use of stem cell-based scaffolds and various optimization strategies.

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Section: Regeneration and Repair Of Skin Woundsmentioning

confidence: 99%

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

Hong

2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

Efficient Gamma‐Retroviral Transduction of Primary Human Skin Cells Using the EF‐c Peptide as a Transduction Enhancer

Abstract: L. (2022). Efficient gamma-retroviral transduction of primary human skin cells using the EF-c peptide as a transduction enhancer. Current Protocols, 2, e353.

Cited by 1 publication

References 32 publications

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

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