Efficient Extraction of Starch from Microalgae Using Ultrasonic Homogenizer and Its Conversion into Ethanol by Simultaneous Saccharification and Fermentation

Abstract

To utilize starch and protein contained in microalgae as carbon and nitrogen sources for ethanol production, an extraction method, i.e. ultrasonic treatment using a homogenizer, and simultaneous saccharification and fermentation (SSF) of extracted microalgae solution were studied using Chlamydomonas fasciata Ettl 437. 30 min of ultrasonic treatment gave the maximum extraction ratio of starch contained in microalgae, i.e. 93.8%, that corresponded to 0.408 g-starch/g-dry microalgae. SSF of the extracted solution obtained from ultrasonic treated microalgae at 30 min by glutase-AN and Saccahromyces cerevisiae AM12 provided 0.194 and 0.168 g-ethanol/g-dry microalgae with and without yeast extract, respectively, corresponding to 79.5 and 68.8% of theoretical ethanol yield.

A mathematical model of intracellular behavior of microalgae for predicting growth and intracellular components syntheses under nutrient‐replete and ‐deplete conditions

“…According to a published paper (Asada, Doi, Sasaki, & Nakamura, ), both chlorophyll and nucleotide, represented as “Functional compartment” in Figure , account for a considerable portion of the total cell weight. It is assumed here that the amount of chlorophyll, , is proportional to the amount of protein (Zhu et al, ) and each cell has a constant amount of nucleotides, of which mass corresponds to .…”

Hydraulic retention time effects on wastewater nutrient removal and bioproduct production via rotating algal biofilm reactor

“…Harvested biofilms in fed-batch mode as same as biomass growth analysis, were dried at 103 o C then, prepared for lipid determination procedure via colorimetric method the same as described by Mishra et al (Mishra et al, 2014) and starch determination using modified methods (Asada et al, 2012;Mooij et al, 2014 …”

Hardness evaluation of dental composites fabricated from the uniform size and well-distributed zirconia-alumina-silica fillers with sol-gel technique

“…During ultrasonic homogenizer process, the longer process causing more vibration of the wave received by the sample and produce smaller size and evenly distributed particle. 20 The particle size and distribution of filler affect the strength and hardness of the sample. Along with the filler , coupling agent is also play important role to link the filler with resin matrix.…”