Efficient extraction of carrageenans from Chondrus crispus for the green synthesis of gold nanoparticles and formulation of printable hydrogels

Álvarez-Viñas, Milena; González-Ballesteros, Noelia; Torres, M.D.; López‐Hortas, Lucía; Vanini, Candida; Domingo, Guido; Rodrı́guez-Argüelles, Marı́a C.; Domı́nguez, Herminia

doi:10.1016/j.ijbiomac.2022.02.145

Cited by 20 publications

(12 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The determination of total protein concentration by the Bradford method revealed that the free-carrageenans extracts obtained at 120 and 160 degrees were the richest in protein content (26.52 and 25.68 g/100 g, respectively; Table S1 ). Our analysis confirmed previous findings [ 21 ] revealing that in the extracts obtained at 160 °C the number of proteins identified was the higher. In fact, after the label-free LC-MS/MS analysis and data processing, a total of 58, 68, 170, 26, and 4 proteins were consistently identified in at least two biological replicates of C. crispus extracts obtained at 120, 140, 160, 180 and 200 °C, respectively ( Table S2 ).…”

Section: Resultssupporting

confidence: 92%

“…According to Alvarez-Vinas and colleagues [ 21 ], C. crispus extracts generated using temperatures of 140 and 160 °C were richest in hydrolyzed proteins. Our gel- and label-free confirmed those results by identifying the largest number of proteins in the 160-degree extract.…”

Section: Discussionmentioning

confidence: 99%

“…The characterization of carrageenan-free extracts from C. crispus used in this study (160-degrees extracts) showed high concentration of hydrolyzed proteins and lower levels of sugars, phenolics and minerals [ 21 ]. Several studies reported the positive effects induced by the applications (both soil and foliar) of plant-derived protein hydrolysates on growth, yield and fruit quality of crops [ 17 , 51 , 52 , 53 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…The hydrolyzed protein-rich extracts were obtained from Chondrus crispus (9.6 ± 0.3 g/100 g of moisture content; Compañía Española de Algas Marinas S.A., CEAMSA, Pontevedra, Spain) according to Alvarez-Viñas et al 2022 [ 21 ]. Briefly, seaweeds were combined with distilled water at a ratio of 30:1 ( w / w ), placed in a pressurized Parr Instruments reactor (series 4842, Moline, IL, USA) and heated during 10–60 min until the selected temperature in the range of 120–200 °C was reached (i.e., 35 min were required in order to attain 160 °C).…”

Section: Methodsmentioning

confidence: 99%

“…Among the most environmentally sustainable processes, the microwave assisted heating was successfully used for the rapid water extraction of carrageenans and other bioactive compounds from Mastocarpus stellatus [ 20 ]. In a previous work [ 21 ], we used a hydrothermal treatment with subcritical water as green extraction technology for the extraction of carrageenans from Chondrus crispus .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Role of Protein-Rich Extracts from Chondrus crispus as Biostimulant and in Enhancing Tolerance to Drought Stress in Tomato Plants

Domingo

Marsoni

Álvarez-Viñas

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

The application of seaweed extract-based biostimulants is a promising approach for achieving sustainable agriculture, with an enormous potential of improving crop yield and mitigating climate change effects. Abiotic stressors, such as drought, are major factors resulting in tomato (Solanum lycopersicum L.) yield losses and seaweed-based biostimulants have been proposed as an eco-friendly strategy to counteract this negative impact. Chondrus crispus is a common red seaweed widely used as source of carrageenans, not yet explored as a plant biostimulant. In this study, a protein hydrolysate-rich C. crispus extract, by-products of the carrageenan extraction, was tested on tomato plants under well-watered condition and water shortage. The foliar application of the protein-rich C. crispus extract conferred drought tolerance to tomato plants resulting in less noticeable visual stress symptoms. Treated plants showed higher shoot height and biomass under both well-watered and water deficit conditions, evidencing the double effect exerted by this new biostimulant, as plant growth promoter and drought stress protector. The treatment with the biostimulant had an effect on levels of abscisic acid and proline, and triggered the expression of Solyc02g084840, a drought marker gene. Finally, a label-free mass spectrometric approach allowed us to identify phycoerythrins and phycocyanins as major bioactive proteins contained in the extract. Altogether, these results indicate that the foliar application of protein hydrolysate-rich extracts from C. crispus improved tomato plant growth and tolerance to drought stress, suggesting a new opportunity for further applications in the agriculture and horticultural sectors.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Role of Protein-Rich Extracts from Chondrus crispus as Biostimulant and in Enhancing Tolerance to Drought Stress in Tomato Plants

Domingo

Marsoni

Álvarez-Viñas

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

Green synthesis of gold nanoparticles using macroalgae Halimeda macroloba extract and their photocatalytic degradation of methylene blue and methyl orange

Lavanya,

Anandaraj,

Selvam

et al. 2024

Polymers for Advanced Techs

View full text Add to dashboard Cite

Green synthesis of gold nanoparticles (AuNPs) is gaining attention of researchers because of their varieties of biomedical and environmental applications. This study reported the novel eco‐friendly synthesis of AuNPs using green macroalgae Halimeda macroloba (HM) extract and evaluate their photocatalytic potential. The green synthesized H. macroloba mediated gold nanoparticles (HM‐AuNPs) was characterized using UV–visible spectrophotometry, Fourier‐transform infrared (FT‐IR) spectroscopy, transmission electron microscope (TEM), scanning electron microscope‐energy dispersive spectroscopy, x‐ray photoelectron spectroscopy (XPS), and particle size distribution (PSD) anlaysis. The UV–visible spectrum shows a sharp intense plasmonic resonance peak at 543 nm. The bioactive compounds present in HM were primarily responsible for the biological reduction of gold ions validated by FT‐IR analysis. XRD analysis proved the crystalline face centered cubic structure of the HM‐AuNPs. The average particle size of 18.72 nm and morphological evidences were obtained from the images from TEM. The metallic form of biosynthesised HM‐AuNPs was confirmed by XPS results with a distinctive binding energy. The photocatalytic degradation ability of the green synthesized HM‐AuNPs was investigated against the methylene blue (MB) and methylene orange (MO) dyes under sunlight irradiation. The HM‐AuNPs exhibited 97.23% and 89.91% photocatalytic activity against MB and MO after 90 min of exposure to sunlight, respectively. The overall results of this research indicate that H. macroloba mediated HM‐AuNPs can be used as an effective option for the degradation of industrial dyes.

show abstract

Green synthesis, characterization, and application of metal oxide nanoparticles for mercury removal from aqueous solution

Gindaba

Demsash²,

Jayakumar³

2022

Environ Monit Assess

View full text Add to dashboard Cite

Efficient extraction of carrageenans from Chondrus crispus for the green synthesis of gold nanoparticles and formulation of printable hydrogels

Cited by 20 publications

References 51 publications

The Role of Protein-Rich Extracts from Chondrus crispus as Biostimulant and in Enhancing Tolerance to Drought Stress in Tomato Plants

The Role of Protein-Rich Extracts from Chondrus crispus as Biostimulant and in Enhancing Tolerance to Drought Stress in Tomato Plants

Green synthesis of gold nanoparticles using macroalgae Halimeda macroloba extract and their photocatalytic degradation of methylene blue and methyl orange

Green synthesis, characterization, and application of metal oxide nanoparticles for mercury removal from aqueous solution

Contact Info

Product

Resources

About