Efficient Enzymatic Enrichment of High-purity Nervonic Acid from <i>Malania oleifera</i> Seed Oil

Abstract

IntroductionNervonic acid (NA; C24:1Δ15; cis-15-tetracosenoic acid) is an ultra-long-chain monounsaturated fatty acid that is widely present in the brain and other neural tissues 1) . This pivotal acid not only contributes to the normal functioning of neural tissues in organisms, but also promotes neurocellular synthesis and brain development in infants 2,3) . Decreased levels of NA are closely associated with several neurological diseases, such as demyelinating disorders, Alzheimer s disease, adrenoleukodystrophy, and depression 4−7) . Consequently, studies have demonstrated NA s potential in in the treatment and prevention of neurodegenerative diseases. However, it is difficult for the body to synthesize NA. Emerging research underscores the benefits of dietary NA supplementation, leading to its incorporation as an active constituent in health foods 8) . Beyond its medical relevance, the versatile NA has found its niche in cosmetic formulations due to its skin-related effects 9) . Given its multifaceted potential across pharmaceutical, nutraceutical, and industrial domains, the demand for high-purity NA is surging, emphasizing the need for consistent and high-quality production.NA was initially isolated from shark brain tissue and also