Efficient elimination of Yam mosaic virus (YMV) from white yam (Dioscorea rotundata Poir.) by cryotherapy of axillary buds

Ita, Effiom Eyo; Uyoh, Edak Aniedi; Nakamura, Ikuo; Ntui, Valentine Otang

doi:10.1016/j.sajb.2019.12.022

Cited by 24 publications

(24 citation statements)

References 21 publications

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“…Virus eradication by cryotherapy is also based on the separation of healthy cells (cell groups) from infected ones [ 75 ], similar to meristem isolation ( Section 2 and Section 2.1 ). During cryotherapy, infected shoot tips or axillary buds are kept in liquid nitrogen at −196 °C for a certain period (in general for 1 h) [ 76 , 77 ]. Differentiated, larger, and more vacuolated cells with high water content survive the ultra-low temperature treatments at a lower rate than smaller, highly cytoplasmic meristem cells [ 74 , 76 , 78 ].…”

Section: Shoot Tip Cryotherapymentioning

confidence: 99%

“…Survival rates are various for different species; it was very low (10%) in apricot ( Prunus armeniaca L.) [ 86 ] while it was 76.3% in yam ( Dioscorea rotunda Poir.) [ 77 ]. Good survival rates could be achieved in apple ( Malus domestica Borkh) (70–75%) [ 87 , 88 ] and up to 85% was obtained in potato ( Solanum tuberosum L.) [ 89 ] and in sweet potato ( Ipomoea batatas (L.) Lam) [ 25 ].…”

Section: Shoot Tip Cryotherapymentioning

confidence: 99%

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Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Magyar-Tábori

Mendler-Drienyovszki

Hanász

et al. 2021

Plants

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In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice.

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Section: Shoot Tip Cryotherapymentioning

confidence: 99%

Section: Shoot Tip Cryotherapymentioning

confidence: 99%

Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Magyar-Tábori

Mendler-Drienyovszki

Hanász

et al. 2021

Plants

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“…Diseases constitute a major constraint to global yam production and are responsible for significant yield losses (Kolombia et al, 2017). Yam viruses have been reported in most of the yam growing areas of West Africa (Odu et al, 1999), causing a reduction in yield and restriction of international exchange of germplasm (Ita et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Severity of Viral Diseases and Types of Weeds as Alternative Viral Hosts in <em>Dioscorea</em> Fields in Southern Guinea Savannah Agroecology of Nigeria

et al. 2021

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The incidence and severity of viruses infecting yam and weeds were determined on 60 Dioscorea farms in Southern Guinea Savannah Agroecology of Kwara State, North-Central Nigeria. Double Antigen Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) was used as a serological technique to detect Yam mosaic virus (YMV) and Cucumber mosaic virus (CMV) on Dioscorea and weeds collected from the farms. The survey indicated virus incidence on Dioscorea between 62.8% and 100% and virus severity between 2 and 5 score values. DAS-ELISA ______________________________________

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“…Several techniques have proved efficient in eliminating viruses from yams, such as chemotherapy, thermotherapy and cryotherapy associated with cell culture [ 21 , 22 ]. Alternative methods have been tested, like hot water treatment on vine cuttings, which resulted in 90% of YMMV elimination [ 23 ], or more recently, treatment with ozone dissolved in water, which resulted in 63.5% of potyvirus elimination [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange

Umber

Filloux

Gélabale

et al. 2020

Viruses

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Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.

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Efficient elimination of Yam mosaic virus (YMV) from white yam (Dioscorea rotundata Poir.) by cryotherapy of axillary buds

Cited by 24 publications

References 21 publications

Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Phytotoxicity and Other Adverse Effects on the In Vitro Shoot Cultures Caused by Virus Elimination Treatments: Reasons and Solutions

Severity of Viral Diseases and Types of Weeds as Alternative Viral Hosts in <em>Dioscorea</em> Fields in Southern Guinea Savannah Agroecology of Nigeria

Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange

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