Efficient Discrimination of Some Moss Species by Fourier Transform Infrared Spectroscopy and Chemometrics

Abstract

Fourier transform infrared spectroscopy (FTIR) technique was used to classify 16 species from three moss families (Mielichhoferiaceae, Bryaceae, and Mniaceae). The FTIR spectra ranging from 4000 cm−1to 400 cm−1of the 16 species were obtained. To group the spectra according to their spectral similarity in a dendrogram, cluster analysis and principal component analysis (PCA) were performed. Cluster analysis combined with PCA was used to give a rough result of classification among the moss samples. However, some species belonging to the same genus exhibited very similar chemical components and similar FTIR spectra. Fourier self-deconvolution (FSD) was used to enhance the differences of the spectra. Discrete wavelet transform (DWT) was used to decompose the FTIR spectra ofMnium laevinerveandM. spinosum. Three scales were selected as the feature extracting space in the DWT domain. Results showed that FTIR spectroscopy combined with DWT was suitable for distinguishing different species of the same genus.

Survival of Moss Reproductive Structures under Simulated Martian Environmental Conditions and Extreme Thermal Stress: Vibrational Spectroscopic Study and Astrobiological Implications

“…Although there are some recent reports of FT-IR spectra [86,87] and Raman spectra [89] for gametophytes of some moss species, this is the first time to our knowledge that FT-Raman and FT-IR spectra are reported for moss spores. As presented in the Introduction section, both species here studied are known to live in open, thermophilous soils, are able to resist heavy disturbance and favour recently burned areas.…”

“…The vibrational bands associated with carbohydrates are predominant the 1200-900 cm -1 spectral region (due to C-O-C, C-OH, C-O stretching and C-C, C-O-H, C-O-C deformation and pyranose and furanose ring vibrations [55,86]. Thus, the peak at 1040 cm -1 can be attributed to oligosaccharides, glycoprotein, and cellulose (C-O stretching) [86,87].…”

“…Absorption bands around 1747 cm -1 correspond to an isolated carbonyl group (COOR), indicating ester-containing compounds, which are commonly found in membrane lipid and cell wall pectin. [86]. The two main groups of lipids, triglycerides and phospholipids are characterized by the strong vibrational band around 1747 cm -1 (C=O stretching) [87].…”

“…Thus, the peak at 1040 cm -1 can be attributed to oligosaccharides, glycoprotein, and cellulose (C-O stretching) [86,87].…”

Biosorption of arsenic (III) from aqueous solution using calcium alginate immobilized dead biomass of Acinetobacter sp. strain Sp2b

“…The FTIR spectra of surface functional groups of the bacterial isolate were analyzed in the following conditions: Kbr method 50 , infrared spectral range of 400–4000 cm –1 , and resolution of 4 cm –1 .…”