Efficient differentiation of stem cells encapsulated in a cytocompatible phospholipid polymer hydrogel with tunable physical properties

Oda, Haruka; Konno, Tomohiro; Ishihara, Kazuhíko

doi:10.1016/j.biomaterials.2015.03.051

Cited by 36 publications

(27 citation statements)

References 22 publications

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“…Considerable research effort has focused on the design of new synthetic 3D matrices for the culture of human stem cells. A range of synthetic vinyl copolymers with various functional groups [9][10][11] as well as alginate 12 and peptide-based 13 hydrogels have been utilized as scaffolds for the continuous proliferation of stem cells. However, care must be taken to control gel stiffness because this parameter can affect stem cell differentiation.…”

Section: Introductionmentioning

confidence: 99%

Probing the mechanism for hydrogel-based stasis induction in human pluripotent stem cells: is the chemical functionality of the hydrogel important?

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Probing the mechanism for hydrogel-based stasis induction in human pluripotent stem cells: is the chemical functionality of the hydrogel important?

et al. 2020

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“…An adaptable hydrogel with appropriate modulus and stress relaxation properties may effectively mimic complex biological tissues . Ishihara and co‐workers reversed the proliferation cycle of cells to G1 phase through the modulation of the storage modulus (G′) of the poly(vinylalcohol)‐based hydrogel, to improve differentiation signal sensitivity which can achieve highly efficient, lineage‐restricted differentiation of stem cells for rapid tissue repairing . More interesting finding by Anseth and co‐workers is that hMSCs can retain the past mechanical information and enough mechanical dosing mediated by a poly(ethylene glycol)‐based photodegradable hydrogel can influence future cell fate decisions regardless of the changing of mechanical environment (Figure e) .…”

Section: Response Of Cells To Robust Mechanical Hydrogel Interfacesmentioning

confidence: 99%

Hierarchical Hydrogel Composite Interfaces with Robust Mechanical Properties for Biomedical Applications

et al. 2019

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Since mechanotransduction and interactions at the interface between materials and cells play crucial roles in the regulation of cell behaviors and the genotype-to-phenotype relation ship, mechanical properties are important parameters in the Cells sense and respond to a wide range of external signals, including chemical signals, topography, and interface mechanics, via interactions with the extracellular matrix (ECM), triggering the regulation of behavior and function. The ECM can be considered a hierarchical multiphase porous matrix with various components. Highly porous hydrogel-based biomaterials can mimic the critical ECM properties, to provide mechanical support for tissues and to regulate cellular behaviors, such as adhesion, proliferation, and differentiation. Herein, based on micro/nanoscale-topography-coupled mechanical action, recent advances in the fabrication and application of hydrogel composites with tunable mechanical properties and topography in biomedicine are summarized. In particular, recent findings showing that hydrogels with specifically designed structures not only influence a range of cellular processes and fit the needs of engineered tissues but also have pharmacological effects are emphasized. Hydrogels

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“…In this study, we adopt an approach in which 11 B/ 13 C NMR spectroscopy and density functional theory (DFT) simulations are combined to elucidate the origin of the high‐affinity binding properties of α‐ or β‐SA–PBA, considering some of the advantages of NMR spectroscopy and DFT generally mentioned in previous work (also see Section S1 in the Supporting Information). Also, 4‐vinylphenylboronic acid (VPBA) was utilized as a model of PBA in the investigation, because it is one of the most commonly used agents in an application incorporating polymers …”

Section: Introductionmentioning

confidence: 99%

“…Also, 4-vinylphenylboronic acid (VPBA) was utilized as am odel of PBA in the investigation, because it is one of the most commonly used agents in an application incorporating polymers. [11,14,27,28] 2. Results and Discussion 2.1.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Molecular Structure of the Sialic Acid–Phenylboronic Acid Complex by using a Combined NMR Spectroscopy and DFT Study: Toward Sialic Acid Detection at Cell Membranes

2018

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The origin of the unusually high stability of the sialic acid (SA) and phenylboronic acid (PBA) complex was investigated by a combined nuclear magnetic resonance (NMR) spectroscopy and density functional theory (DFT) study. SA is a glycan‐terminating monosaccharide, and its importance as a clinical target has long been recognized. Inspired by the fact that the binding properties of SA–PBA complexation are anomalously high relative to those of typical monosaccharides, great effort has been made to build a clinical platform with the use of PBA as a SA‐selective receptor. Although a number of applications have been reported in recent years, the ability of PBA to recognize SA‐terminating surface glycans selectively is still unclear, because high‐affinity SA–PBA complexation might not occur in a physiological environment. In particular, different forms of SA (α‐ and β‐pyranose) were not considered in detail. To answer this question, the combined NMR spectroscopy/DFT study revealed that the advantageous binding properties of the SA–PBA complex arise from ester bonding involving the α‐carboxylate moieties (C1 and C2) of β‐SA but not α‐SA. Moreover, the facts that the C2 atom is blocked by a glycoside bond in a physiological environment and that α‐SA basically exists on membrane‐bound glycans in a physiological environment lead to the conclusion that PBA cannot selectively recognize the SA unit to discriminate specific types of cells. Our results have a significant impact on the field of SA‐based cell recognition.

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Efficient differentiation of stem cells encapsulated in a cytocompatible phospholipid polymer hydrogel with tunable physical properties

Cited by 36 publications

References 22 publications

Probing the mechanism for hydrogel-based stasis induction in human pluripotent stem cells: is the chemical functionality of the hydrogel important?

Probing the mechanism for hydrogel-based stasis induction in human pluripotent stem cells: is the chemical functionality of the hydrogel important?

Hierarchical Hydrogel Composite Interfaces with Robust Mechanical Properties for Biomedical Applications

Understanding the Molecular Structure of the Sialic Acid–Phenylboronic Acid Complex by using a Combined NMR Spectroscopy and DFT Study: Toward Sialic Acid Detection at Cell Membranes

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