Efficient differentiation of CD14+ monocytic cells into endothelial cells on degradable biomaterials

Krenning, Guido; Dankers, Patricia Y. W.; Jovanović, Danijela; Luyn, Marja J. A. van; Harmsen, Martin C.

doi:10.1016/j.biomaterials.2006.11.017

Cited by 41 publications

(35 citation statements)

References 35 publications

(35 reference statements)

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“…60 Depending on the biological material and stimulus, monocytes develop to Ͼ75% into endothelial-like cells and only to Ϸ15% to 25% in macrophages. 61 Additional experiments revealed that a CD14/KDR ϩ subset may preferentially "transdifferentiate" into endothelial-like cells. Interestingly, endothelial-like cells not only look like ECs, they seem to behave like true ECs.…”

Section: Separated Cd14mentioning

confidence: 99%

Endothelial-Regenerating Cells

2010

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Abstract-Atherosclerosis is the most common cause for cardiovascular diseases and is based on endothelial dysfunction.A growing body of evidence suggests the contribution of bone marrow-derived endothelial progenitor cells, monocytic cells, and mature endothelial cells to vessel formation and endothelial rejuvenation. To this day, various subsets of these endothelial-regenerating cells have been identified according to cellular origin, phenotype, and properties in vivo and in vitro. However, the definition and biology, especially of endothelial progenitor cells, is complex and under heavy debate. In this review, we focus on current definitions of endothelial progenitor cells, highlight the clinical relevance of endothelial-regenerating cells, and provide new insights into cell-cell interactions involved in endothelial cell rejuvenation. (Hypertension. 2010;55:593-599.)

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Section: Separated Cd14mentioning

confidence: 99%

Endothelial-Regenerating Cells

2010

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“…were isolated from peripheral blood by density-gradient centrifugation of Lymphoprep (Nycomed Pharma) as previously described (19,21). Aliquots of 1 ϫ 10 6 MNC were subsequently labeled using monoclonal antibodies to the EPC markers CD34 (BD Pharmingen, San Jose, CA) and CD14 (IQ Products, Groningen, The Netherlands), and EPC were quantified by flow cytometry (BD Biosciences).…”

Section: Isolation and Quantification Of Epc Mononuclear Cells (Mnc)mentioning

confidence: 99%

“…EPC are a self-renewing cell population present in the bone marrow and circulation, which can differentiate into functional endothelial cells in vitro and in vivo (15). We and others have identified two distinct EPC subsets, the CD34 ϩ EPC (1,27) and the CD14 ϩ EPC (19,29), in the peripheral blood of healthy subjects. These EPC subsets have been proposed and applied as tools in, among others, cell therapy for ischemic diseases (18,22), and the generation of bioartificial tissues, such as endothelialized antithrombogenic hemodialysis access (24) or replacement blood vessels (17,20).…”

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confidence: 97%

Endothelial progenitor cell dysfunction in patients with progressive chronic kidney disease

Krenning¹,

Dankers²,

Drouven³

et al. 2009

American Journal of Physiology-Renal Physiology

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Endothelial progenitor cells (EPC) contribute to repair and maintenance of the vascular system, but in patients with chronic kidney disease (CKD), the number and function of EPC may be affected by kidney dysfunction. We assessed numbers and the angiogenic function of EPC from patients with CKD in relation to disease progression. In a cross-sectional, prospective study, 50 patients with varying degrees of CKD, including 20 patients undergoing dialysis and 10 healthy controls, were included. Mononuclear cells were isolated, and circulating EPC were quantified by flow cytometry based on expression of CD14 and CD34. EPC were cultured on fibronectin-coated supramolecular films of oligocaprolactone under angiogenic conditions to determine their angiogenic capacity and future use in regenerative medicine. CKD patients had normal numbers of circulating CD14+ EPC but reduced numbers of circulating CD34+ EPC. Furthermore, EPC from patients with CKD displayed functional impairments, i.e., hampered adherence, reduced endothelial outgrowth potential, and reduced antithrombogenic function. These impairments were already observed at stage 1 CKD and became more apparent when CKD progressed. Dialysis treatment only partially ameliorated EPC impairments in patients with CKD. In conclusion, EPC number and function decrease with advancing CKD, which may hamper physiological vascular repair and can add to the increased risk for cardiovascular diseases observed in CKD patients.

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“…Monocytes/macrophages display a high degree of plasticity as demonstrated by their ability to transdifferentiate into endothelial cells, EPCs or endotheliallike cells (ELCs), both in vitro and in vivo (Kim et al 2009;Krenning et al 2007;Rehman et al 2003;Rohde et al 2006). These findings indicate a potential developmental relationship between monocytes/macrophages and endothelial cells and suggest that the monocytes/macrophages may represent a population of endothelial precursor cells and would be an appropriate candidate source for endothelial cells, EPCs or ELCs.…”

Section: Introductionmentioning

confidence: 92%

Macrophages overexpressing VEGF, transdifferentiate into endothelial-like cells in vitro and in vivo

Yan

Wang

et al. 2011

Biotechnol Lett

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Vascular endothelial growth factor (VEGF) induces endothelial cell differentiation. To investigate the role of VEGF overexpression in regulating the phenotype of macrophages, we transfected mouse macrophages with human VEGF(165) and examined the expression of the genes and proteins for various endothelial markers. Macrophages overexpressing VEGF significantly expressed fetal liver kinase 1 (FLK-1), vascular endothelial cadherin (VE-cadherin), CD31, Von Willebrand factor (vWF), endothelial nitric oxide synthase (eNOS) and CD105. Furthermore, in a model of myocardial infarction (MI), macrophages overexpressing VEGF incorporated into blood vessels. Thus macrophages overexpressing VEGF were transdifferentiate into endothelial-like cells (ELCs) both in vitro and in vivo.

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Efficient differentiation of CD14+ monocytic cells into endothelial cells on degradable biomaterials

Cited by 41 publications

References 35 publications

Endothelial-Regenerating Cells

Endothelial-Regenerating Cells

Endothelial progenitor cell dysfunction in patients with progressive chronic kidney disease

Macrophages overexpressing VEGF, transdifferentiate into endothelial-like cells in vitro and in vivo

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