Efficient differentiation of beige adipocytes from adult human adipose-derived stem/stromal cells

Singh, Arashdeep; Zhang, Liang; Avery, John; Dalton, Stephen

doi:10.21203/rs.3.pex-888/v1

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2020

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“…A step-by-step protocol describing the generation of beige adipocytes from ADSCs can be found at Protocol Exchange (ref. 69 ).…”

Section: Methodsmentioning

confidence: 99%

Human beige adipocytes for drug discovery and cell therapy in metabolic diseases

et al. 2020

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Human beige adipocytes (BAs) have potential utility for the development of therapeutics to treat diabetes and obesity-associated diseases. Although several reports have described the generation of beige adipocytes in vitro, their potential utility in cell therapy and drug discovery has not been reported. Here, we describe the generation of BAs from human adiposederived stem/stromal cells (ADSCs) in serum-free medium with efficiencies >90%. Molecular profiling of beige adipocytes shows them to be similar to primary BAs isolated from human tissue. In vitro, beige adipocytes exhibit uncoupled mitochondrial respiration and cAMP-induced lipolytic activity. Following transplantation, BAs increase whole-body energy expenditure and oxygen consumption, while reducing bodyweight in recipient mice. Finally, we show the therapeutic utility of BAs in a platform for high-throughput drug screening (HTS). These findings demonstrate the potential utility of BAs as a cell therapeutic and as a tool for the identification of drugs to treat metabolic diseases.

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“…A step-by-step protocol describing the generation of beige adipocytes from ADSCs can be found at Protocol Exchange (ref. 69 ).…”

Section: Methodsmentioning

confidence: 99%

Human beige adipocytes for drug discovery and cell therapy in metabolic diseases

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

Efficient differentiation of beige adipocytes from adult human adipose-derived stem/stromal cells

Cited by 1 publication

References 5 publications

Human beige adipocytes for drug discovery and cell therapy in metabolic diseases

Human beige adipocytes for drug discovery and cell therapy in metabolic diseases

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