Efficient deletion of microRNAs using CRISPR/Cas9 with dual guide RNAs
Smitha Ijee,
Karthik Chambayil,
Anurag Dutta Chaudhury
et al.
Abstract:MicroRNAs (miRNAs) are short non-coding RNAs that play crucial roles in gene regulation, exerting post-transcriptional silencing, thereby influencing cellular function, development, and disease. Traditional loss-of-function methods for studying miRNA functions, such as miRNA inhibitors and sponges, present limitations in terms of specificity, transient effects, and off-target effects. Similarly, CRISPR/Cas9-based editing of miRNAs using single guide RNAs (sgRNAs) also has limitations in terms of design space f… Show more
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