Efficient decellularization for bovine pericardium with extracellular matrix preservation and good biocompatibility

Li, Ning; Yang, Li; Gong, Daozhi; Xia, Cuiping; Liu, Xiaohong; Xu, Zhiyun

doi:10.1093/icvts/ivx416

Cited by 60 publications

(54 citation statements)

References 24 publications

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“…In similar settings, this biomechanical property appeared to be strongly reduced by GA-treatment (e.g., elastic phase), by confirming previously revealed rigidity of these clinically applied biomaterials [34,55,66]. As generally documented [14,66,67], a similar ability of decellularized bovine pericardium to withstand tensile load as its native counterpart -differently from porcine one -is apparent [55][56][57]. These aptitudes seem to be intrinsically related to a species-specific biomechanical response of analyzed tissues, since they were observed also after the application of different extraction protocols.…”

Section: Discussionsupporting

confidence: 84%

“…GA-treated pericardium, as applied in several reconstructive surgeries, is known to be poorly cytocompatible [69] and no comprehensive data are available with regard to ISO 10993-5 evaluations for commercial decellularized pericardia already used in the clinics, although declared biocompatible. In most cytocompatibility assays, murine immortalized lines, e.g., NIH/3T3 or L929, or other animals-derived cells are preferred [55,66,67]. In our investigation, we specifically selected human cytotypes to simulate the effects that are exerted by decellularized scaffolds in the in vivo human setting.…”

Section: Discussionmentioning

confidence: 99%

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A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

et al. 2020

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Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellularization, ideally removing cells and preserving the original extracellular matrix (ECM). The aim of this study was to compare the mostly applied pericardia in clinics, i.e., bovine and porcine tissues, after their decellularization, and obtain new insights for their possible surgical use. Bovine and porcine pericardia were submitted to TRICOL decellularization, based on osmotic shock, detergents and nuclease treatment. TRICOL procedure resulted in being effective in cell removal and preservation of ECM architecture of both species’ scaffolds. Collagen and elastin were retained but glycosaminoglycans were reduced, significantly for bovine scaffolds. Tissue hydration was varied by decellularization, with a rise for bovine pericardia and a decrease for porcine ones. TRICOL significantly increased porcine pericardial thickness, while a non-significant reduction was observed for the bovine counterpart. The protein secondary structure and thermal denaturation profile of both species’ scaffolds were unaltered. Both pericardial tissues showed augmented biomechanical compliance after decellularization. The ECM bioactivity of bovine and porcine pericardia was unaffected by decellularization, sustaining viability and proliferation of human mesenchymal stem cells and endothelial cells. In conclusion, decellularized bovine and porcine pericardia demonstrate possessing the characteristics that are suitable for the creation of novel scaffolds for reconstruction or replacement: differences in water content, thickness and glycosaminoglycans might influence some of their biomechanical properties and, hence, their indication for surgical use.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

et al. 2020

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“…In the light of new studies on hepatic tissue engineering to find an optimal procedure for whole liver decellularization, recellularization, and regeneration during recent years, many researchers are trying to obtain a decellularized liver ECM (DLM) with several detergents such as sodium dodecyl sulfate (SDS) and Triton X‐100. SDS, with a hydrophobic chain and a charged headgroup, as a strong and harsh detergent lyses cell membrane and solves the structural proteins by interruption of noncovalent bonds between proteins, while Triton X‐100 with uncharged headgroup and hydrophilic chain is not as effective as SDS, but it preserves ECM molecules in the decellularized tissue more intact (Li et al, ). Sodium lauryl ether sulfate (SLES), an anionic and novel detergent in field of decellularization, is newly proposed in literature (Hassanpour, Talaei‐Khozani, Kargar‐Abarghouei, Razban, & Vojdani, ; Kawasaki et al, ; Ma et al, ) and selected in this study for rat liver decellularization.…”

Section: Introductionmentioning

confidence: 99%

Decellularized liver transplant could be recellularized in rat partial hepatectomy model

Naeem

Daneshi

Talaei‐Khozani

et al. 2019

J Biomedical Materials Res

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In situ recellularization of the liver decellularized scaffold is a potential therapeutic alternative for liver transplantation. We aimed to develop an in situ procedure for recellularization of the rat liver using sodium lauryl ether sulfate (SLES) compared with Triton X-100/SDS. Rat liver specimens were rinsed with PBS, decellularized with either Triton X-100/SDS or SLES, and finally rinsed by distilled water. The efficiency of decellularized liver scaffolds was evaluated by histological, confocal Raman microscopy, histochemical staining, and DNA quantification assessments. Finally, in vivo studies were done to assess the biocompatibility of the liver scaffold by serum biochemical parameters and the recellularization capacity by histological and immunohistochemistry staining. Findings confirmed the preservation of extracellular matrix (ECM) components such as reticular, collagen, glycosaminoglycans, and neutral carbohydrates in both Triton X-100/SDS-and SLES-treated livers. Hoechst, feulgen, Hematoxylin and eosin, and DNA quantification assessments confirmed complete genetic content removal. The serological parameters showed no adverse impact on the liver functions. Transplantation of SLES-treated cell-free decellularized liver showed extensive neovascularization along with migration of the fibrocytes and adipocytes and some immune cells. Also, immunohistochemical staining showed that the oval cells, stellate cells, cholangiocytes and hepatocytes invaded extensively into the graft. It is concluded that SLES can be considered as a promising alternative in the liver decellularization process, and the transplanted decellularized liver can appropriately be revascularized and regenerated. K E Y W O R D S decellularization, liver, scaffold, sodium lauryl ether sulfate

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“…Gels derived from dECM bladder were prepared as stated earlier in 24‐well plates. HUVECs were chosen as an ideal human cell type for testing due to their extensive usage in tissue engineering applications with decellularized scaffolds to promote angiogenesis and tissue regeneration . HUVECs were seeded at a density of 40,000 cells per well and cultured for 72 h. For live‐dead staining and microscopy, cells were incubated with 2 µM Calcein AM live stain and 4 µM EthD‐1 dead stain in PBS (1×; pH 7.4) with calcium and magnesium for 45 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

Szulc

Ahmadipour

Aoki

et al. 2019

Magnetic Resonance in Med

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Purpose To develop a facile method for labeling and imaging decellularized extracellular matrix (dECM) scaffolds intended for regenerating 3D tissues. Methods A small molecule manganese porphyrin, MnPNH2, was synthesized and used to label dECM scaffolds made from porcine bladder and trachea and murine whole lungs. The labeling protocol was optimized on bladder dECM, and imaging on a 3T clinical scanner was performed to assess reductions in T1 and T2 relaxation times. In vivo MRI was performed on dECM injected in the rat dorsum to verify sensitivity of detection. Toxicity assays for cell viability, metabolism, and proliferation were performed on human umbilical vein endothelial cells. The incorporation of MnPNH2 and its long‐term retention in dECM were assessed on transmission electron microscopy and ultraviolet absorbance of eluted MnPNH2 over time. Results All tissues, including thick whole 3D organs, were uniformly labeled and demonstrated high signal‐to‐noise on MRI. A nearly 10‐fold reduction in T1 was consistently obtained at a labeling dose of 0.4 mM, and even 0.2 mM provided sufficient contrast in vivo and ex vivo. No toxicity was observed up to 0.4 mM, the maximum tested. Binding studies suggested nonspecific association, and retention studies in the labeled whole decellularized lungs revealed less than 20% MnPNH2 loss over 30 days, the majority occurring in the first 3 days after labeling. Conclusion The proposed labeling method is the first report for visualizing dECM on MRI and has the potential for long‐term monitoring and optimization of dECM‐based organ tissue engineering.

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Efficient decellularization for bovine pericardium with extracellular matrix preservation and good biocompatibility

Abstract: These results suggested that the FTS protocol showed optimal decellularization results with better extracellular matrix preservation and good biocompatibility. It may be a suitable protocol for producing a suitable scaffold for heart tissue engineering.

Cited by 60 publications

References 24 publications

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

A Comprehensive Comparison of Bovine and Porcine Decellularized Pericardia: New Insights for Surgical Applications

Decellularized liver transplant could be recellularized in rat partial hepatectomy model

MRI method for labeling and imaging decellularized extracellular matrix scaffolds for tissue engineering

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