Efficient conversion of extracts from low-cost, rejected fruits for high-valued Docosahexaenoic acid production by Aurantiochytrium sp. SW1

Nazir, Yusuf; Halim, Hafiy; Al-Shorgani, Najeeb Kaid Nasser; Manikan, Vidyah; Hamid, Aidil Abdul; Song, Yuanda

doi:10.1016/j.algal.2020.101977

Cited by 23 publications

(9 citation statements)

References 61 publications

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“…After an in-depth analysis of the corn steep liquor composition, it was found that with the addition of the sugars contained in the corn steep liquor, the medium reached a sugar concentration of 70 g/L. This result is consistent with the studies of Nazir et al (2020;, who showed that the optimal carbon source concentration for the growth and DHA production of Aurantiochytrium SW1 is in the range of 60-100 g/L and the growth of Aurantiochytrium SW1 is inhibited with carbon sources above 100 g/L.…”

Section: Optimization Of Mficroalgal Biomasssupporting

confidence: 88%

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Ren¹,

Qi²,

Liu³

et al. 2021

American Journal of Biochemistry and Biotechnology

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Thraustochytrium has a relatively high lipid content in the cell and its lipid content can account up to 70% of the dry cell weight. It is an important raw material for the third-generation biodiesel production and the production of functional fatty acid Docosahexaenoic Acid (DHA). However, the high cost of traditional fermentation medium has hindered the industrial production of lipid. Therefore, searching for raw materials that can be used by thraustochytrid with a wide range of sources and low prices has become a research hotspot in the production of lipids by thraustochytrid. In this study, corn steep liquor was applied for lipid production in thraustochytrid Aurantiochytrium SW1, various factors of corn medium were optimized by using Response Surface Methodology (RSM), including glucose concentration, Rotation Speed, Kinetin concentration and dilution rate of corn steep liquor. The optimal conditions were finally obtained as 72.37 g/L glucose, 2.28 mg/L kinetin, 270 rpm of rotation speed and 15 times of corn pulp dilution. The biomass, lipid and DHA yields obtained under the optimized conditions were 13.5, 7.9 and 42% of total fatty acids, respectively. Comparison with the traditional medium, the biomass, lipid and DHA production were promoted by 14.8, 31.6 and 31.3% respectively. The optimized medium using cheap corn steep liquor showed a better production level, which proved the feasibility of culturing Aurantiochytrium SW1 with cheap raw materials.

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Section: Optimization Of Mficroalgal Biomasssupporting

confidence: 88%

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Ren¹,

Qi²,

Liu³

et al. 2021

American Journal of Biochemistry and Biotechnology

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“…In this study, the C/N ratio for RL is 3 and for RC 2.8. Nazir et al. (2020) using Ananas comosus extract as a substrate for Aurantiochitryum sp.…”

Section: Discussionmentioning

confidence: 99%

“…AH-2 produced 23.74% of DHA (Thyagarajan et al, 2014) with bread crumbs in submerged substrate and Thraustochytrium Kinney VAL-B1 generated 35.06% of DHA (Silva et al, 2017) with residual glycerol. Recently Nazir et al (2020) generated extracts with rejected pineapple and banana fruits, and used them as a substrate for Aurationchytrium SW1 producing 44.86% DHA, being one of the highest values found so far. For this reason, the culture of VAL-B1 using waste instead of pure carbon sources could be a good alternative for producing DHA at low cost, since the utilization of pure carbon source, increase the cost of the industrial production processes.…”

Section: Determination Of Fatty Acid Composition In the Various Cultumentioning

confidence: 99%

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Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Quilodrán

Cortínez

Bravo

et al. 2020

Heliyon

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The thraustochytrid are marine heterotrophic protists that are widely distributed in the marine world. They are characterized by producing and accumulating great amount of lipids in their cells, especially long chain polyunsaturated fatty acids (LC-PUFA), highlighting the docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3) and arachidonic acid (ARA, 20:4, n-6), as well as pigments of interest for human health and animal nutrition, such as carotenoids. Therefore, the objective of this study was to isolate and characterize three natives isolated of thraustochytrids and assess the potential of the by-products of the manufacture of beer (RB) and protein extraction of Lupine flour (RL) as complex carbon sources to produce biomass, lipid and polyunsaturated fatty acids. Three native strains of thraustochytrid (AS5-B2, IQ81 y VAL-B1), isolated from Chilean coastal waters were morphologically and genetically identified as thraustochytrid. For the determination of biomass production cultures were quantified by gravimetry and the fatty acids quantification and identification were carried out by gas chromatography (GC-FID). Our results show that the culture with any sources of complex carbon used, increased significantly the production of both biomass and total lipids in the strains IQ81 and VAL-B1, compared to glucose as pure carbon source. On the other hand, strain AS5-B2 showed a decrease in the total production of lipids in RB compared to the pure carbon source. For the production of fatty acids, the strains IQ81 and VAL-B1 showed a significant increase in DHA when growing in RB. In conclusion strains IQ81 and VAL-B1 can be used to biotransform industrial waste, such as RB and RL, into a more valuable product such as DHA, EPA, ARA and lipids.

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“…This could be related to their vital functions in the lipid biosynthesis pathway and growth. An increment in the concentration of carbon sources (particularly fructose in this study) is known to enhance lipid production in all oleaginous microorganisms including thraustochytrids as it provides excess continuous carbon flux in the central carbon backbone which then enters the lipid biosynthesis pathway upon nitrogen limitation is reached [ 28 ]. On the other hand, yeast extract provides essential vitamins particularly B complex which is vital for cell growth, thus directly correlates to its positive effects on the DHA volumetric content by positively influencing biomass concentration.…”

Section: Discussionmentioning

confidence: 99%

Two-level factorial analysis of the effect of fructose on DHA biosynthetic capacity of Aurantiochytrium sp. SW1

Manikan

Nazir

Hamid

2021

Heliyon

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Thraustochytrids are getting increasingly popular due to their high potential role as alternative producers of the high-valued ω-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA). While most thraustochytrids prefer glucose as the major carbon source, few strains have been reported to prefer fructose. One such strain is Aurantiochytrium sp. SW1. In this study, the effect of fructose on DHA accumulation by SW1 was investigated using a two-level full factorial design. Besides, biomass, lipid and DHA accumulation profiles of SW1 cultivated in fructose and glucose media were compared. Results revealed that fructose has a very significant positive effect on the volumetric DHA content. Meanwhile, its involvement in affecting DHA biosynthetic capacity, though significant, is not very profound. It was also found that when cultivated in fructose medium, SW1 had a less steep log phase compared to that of glucose medium. However, after 48h of cultivation, biomass and lipid accumulation in fructose medium outweighed the other. Volumetric DHA content in fructose medium at 96h was 11% higher than that of glucose medium. Overall, fructose was found to be a more suitable substrate for biomass, lipid and DHA accumulation in SW1 compared to the conventional source, glucose.

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Efficient conversion of extracts from low-cost, rejected fruits for high-valued Docosahexaenoic acid production by Aurantiochytrium sp. SW1

Cited by 23 publications

References 61 publications

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Producing Functional Fatty Acid DHA from Corn Steep Liquor by Aurantiochytrium SW1

Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Two-level factorial analysis of the effect of fructose on DHA biosynthetic capacity of Aurantiochytrium sp. SW1

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