Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

Wang, Zhipeng; Wang, Qin‐Qing; Liu, Song; Liu, Xiaofang; Yu, Xinjun; Jiang, Yupeng

doi:10.3390/molecules24071228

Cited by 30 publications

(18 citation statements)

References 36 publications

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“…Thus, people began to use glutamate to enhance the yield of this polymer, resulting in a significant increase of the cost. To produce γ-PGA at a low cost, we tested whether FA powder, which is produced from the wastewater of molasses fermentation in the yeast fermentation industry [ 29 ], could substitute some nutrients in the original fermentation medium formula. We first determined whether GJ11 was compatible to FA powder.…”

Section: Discussionmentioning

confidence: 99%

Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Wang

Liu

et al. 2020

Biotechnol Biofuels

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Background Molasses is a wildly used feedstock for fermentation, but it also poses a severe wastewater-disposal problem worldwide. Recently, the wastewater from yeast molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied into soil. In this study, the low-cost FA powder was bioconverted into a value-added product of γ-PGA by a glutamate-independent producer of Bacillus velezensis GJ11. Results FA power could partially substitute the high-cost substrates such as sodium glutamate and citrate sodium for producing γ-PGA. With FA powder in the fermentation medium, the amount of sodium glutamate and citrate sodium used for producing γ-PGA were both decreased around one-third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+, and Fe3+ in the fermentation medium for producing γ-PGA. In the optimized medium with FA powder, the γ-PGA was produced at 42.55 g/L with a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g., H2O2 accumulation and callose deposition, against the pathogen’s infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolysates was dependent on the ethylene (ET) signaling and nonexpressor of pathogenesis-related proteins 1 (NPR1). Conclusions To our knowledge, this is the first report to use the industry waste, FA powder, as a sustainable substrate for microbial synthesis of γ-PGA. This bioprocess can not only develop a new way to use FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

show abstract

Section: Discussionmentioning

confidence: 99%

Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Wang

Liu

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…In the industrial bioprocess, molasses is a cheap and widely used carbon source for microbial fermentation, which can be used to produce various kinds of highly valuable metabolites [29]. However, the wastewater produced during molasses fermentation can lead to an environmental pollution if it is directly discharged into the environment without appropriate pre-treatment.…”

Section: Discussionmentioning

confidence: 99%

Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

Li¹,

Wang²,

Ke³

et al. 2020

Preprint

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Background: Molasses is a wildly used feedstock for fermentation, but it poses a severe wastewater-disposal problem worldwide. Recently, the wastewater produced by yeast during molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied in soil. In this study, the low-cost FA powder was bioconverted into a value-added product, γ-PGA, by a glutamate independent producer, Bacillus velezensis GJ11. Results: With FA powder, the substrates of sodium glutamate and citrate sodium used in medium were decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium. In the optimized FA powder fermentation medium, the γ-PGA was produced with its maximum concentration at 42.55 g/L and a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolyzates was dependent on the ethylene signalling and NPR1. Conclusions: To our knowledge, this is the first report of using the industry waste, FA powder, as a sustainable substrate for the microbial synthesis of γ-PGA. This bioprocess can not only develop a new way of FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

show abstract

“…Thus, people began to use glutamate to enhance the yield of this polymer, resulting in a signi cant increase of the cost. In order to produce γ-PGA at a low cost, we tested whether FA powder, which is produced from the wastewater of molasses fermentation in the yeast fermentation industry [29], could substitute some nutrients in the original fermentation medium formula. We rstly determined whether GJ11 was compatible to FA powder.…”

Section: Discussionmentioning

confidence: 99%

Microbial synthesis of poly- γ -glutamic acid ( γ -PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Wang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Molasses is a wildly used feedstock for fermentation, but it also poses a severe wastewater-disposal problem worldwide. Recently, the wastewater from yeast molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied into soil. In this study, the low-cost FA powder was bioconverted into a value-added product of γ-PGA by a glutamate independent producer of Bacillus velezensis GJ11.Results: FA power could partially substitute the high-cost substrates such as sodium glutamate and citrate sodium for producing γ-PGA. With FA powder in the fermentation medium, the amount of sodium glutamate and citrate sodium used for producing γ-PGA were both decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium for producing γ-PGA. In the optimized medium with FA powder, the γ-PGA was produced at 42.55 g/L with a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen’s infection in plants. Further investigations found the ISR triggered by γ-PGA hydrolysates was dependent on the ethylene (ET) signalling and nonexpressor of pathogenesis-related proteins 1 (NPR1). Conclusions: To our knowledge, this is the first report to use the industry waste, FA powder, as a sustainable substrate for microbial synthesis of γ-PGA. This bioprocess can not only develop a new way to use FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

show abstract

Efficient Conversion of Cane Molasses Towards High-Purity Isomaltulose and Cellular Lipid Using an Engineered Yarrowia lipolytica Strain in Fed-Batch Fermentation

Cited by 30 publications

References 36 publications

Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

Microbial synthesis of poly- γ -glutamic acid ( γ -PGA) with fulvic acid powder, the waste from yeast molasses fermentation

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