“…The insertion of amino acid moieties, bare or functionalized, in their structure has been generally more used in pDNA transfection [ 18 , 20 , 22 , 24 , 25 , 26 , 27 , 28 ] than in siRNA vectorization [ 29 , 30 , 31 , 32 ], despite the fact that amphiphilic imidazolium salt has already been physico-chemically presented as a new generation of reagents for RNAi [ 33 ]. Additionally, gemini cationic lipids (GCL) have demonstrated to be efficient gene nanovectors [ 34 , 35 , 36 ], especially when an imidazolium group was included in their structure [ 37 , 38 ]. Both synthetic strategies are focused on overcoming the biological barriers that the nanocarrier encounters once it enters the bloodstream [ 39 ], as well as on improving the endocytosis pathway [ 40 ].…”