Efficient biodegradation of acephate by Pseudomonas pseudoalcaligenes PS-5 in the presence and absence of heavy metal ions [Cu(II) and Fe(III)], and humic acid

Abstract

The present study was intended to investigate the biodegradation of acephate in aqueous media in the presence and in the absence of metal ions [Fe(III) and Cu(II)], and humic acid (HA). Biodegradations were performed using PS-5 (PS-5) isolated from the heavy metal polluted site. Biodegradations were monitored by UV-Visible, FTIR, and electron spray ionization-mass spectrometry (ESI-MS) analyses. ESI-MS analysis revealed that PS-5 degraded acephate to two metabolites showing intense ions at mass-to-charge ratios (/) 62 and 97. The observed kinetic was the pseudo-first order, and half-life periods () were 2.79 d (of PS-5 + acephate), 3.45 d [of PS-5 + acephate + Fe(III)], 3.16 d [of PS-5 + acephate + Cu(II)], and 5.54 d (of PS-5 + acephate + HA). A significant decrease in degradation rate of acephate was noticed in the presence of HA, and the same was confirmed by UV-Visible and TGA analyses. Strong aggregation behavior of acephate with humic acid in aqueous media was the major cause behind the slow degradation rate of acephate . New results on acephate metabolism by strain PS-5 in the presence and in the absence of metal ions [Fe(III) and Cu(II)] and humic acid were obtained. Results confirmed that strain PS-5 was capable of mineralization of the acephate without formation of toxic metabolite methamidophos. More significantly, the strain PS-5 could be useful as potential biological agents in effective bioremediation campaign for multi-polluted environments.

Spectral, structural and energetic study of acephate, glyphosate, monocrotophos and phorate: an experimental and computational approach

“…These studies are applicable to all the molecules containing secondary amine [60][61][62][63]. After the derivatization, UV-visible analysis can be used to detect these molecules from various compartments of the environment [54][55][56][57][58][59][60][61][62][63]. In our recent study, we have found that acephate and glyphosate were detectable by UV-visible, FTIR, mass and NMR studies [60,63].…”

“…These studies are very important, especially with environmental concern and real-world analysis applications. For example, the detection of glyphosate by UV-visible spectrophotometric methods which are reliable and belongs to real-time analysis, interactions of pesticides with the soils and their adsorption on clay and humates [41][42][43][44][45][46][47][48][49][50][54][55][56][57][58][59][60][61][62][63]. These studies are the foundation of detection of various contaminants and their metabolites through the chromatographic methods in water, soils, fruits, crops, vegetables and other samples [41][42][43][44][45][46][50][51][52][53][54][55][56]64].…”

“…In literature, the analysis of the glyphosate has been mentioned, where derivatization done was analysed by UVvisible and HPLC methods only [53][54][55]64]. These studies are applicable to all the molecules containing secondary amine [60][61][62][63]. After the derivatization, UV-visible analysis can be used to detect these molecules from various compartments of the environment [54][55][56][57][58][59][60][61][62][63].…”

Assessment of antioxidant system and enzyme/nonenzyme regulation related to ascorbate-glutathione cycle in ferulic acid-treated Triticumaestivum L. roots under boron toxicity

“…These results are consistent with the data obtained by Abu El-Soud et al (2013) who indicated that the application of ellagic acid to chickpea plants mitigated effects of stress by reducing ROS content. Moreover, some of the recent studies showed that the application of phenolic acids such as gallic and cinnamic acid alleviated the oxidative damage induced by stress via lessening H 2 O 2 and TBARS contents and increasing antioxidant capacity (Ozfidan-Konakci et al, 2015;Singh et al, 2017a).…”

Kinetic Study of the Biodegradation of Acephate by Indigenous Soil Bacterial Isolates in the Presence of Humic Acid and Metal Ions

“…These are costly, time-consuming, and inefficient techniques and can lead to the formation of toxic metabolites, i.e., side-products of pesticides. On the other side, the use of microorganisms is an effective way to decompose pesticides in a green or eco-friendly manner [7]. These microbe-based techniques are efficient in eradicating hazardous compounds from different ecosystems.…”