Efficient bioconversion of furfural to furfuryl alcohol byBacillus coagulansNL01

Abstract

Bio-catalytic reduction of furfural to furfuryl alcohol was successfully carried out by Bacillus coagulans NL01 whole-cells in the presence of glucose.

Efficient whole‐cell biotransformation of furfural to furfuryl alcohol by Saccharomyces cerevisiae NL22

“…In contrast, whole‐cell biocatalysis shows a higher FAL tolerance and productivity. Therefore, 50 mmol L −1 and 0.03 mmol h −1 for Meyerozyma guilliermondii SC1103 and 112 mmol L −1 FAL and 0.10 mmol h −1 for B. coagulans NL01, respectively . The substrate glucose is two‐fold higher in He et al .…”

“…Therefore, 50 mmol L −1 and 0.03 mmol h −1 for Meyerozyma guilliermondii SC1103 and 112 mmol L −1 FAL and 0.10 mmol h −1 for B. coagulans NL01, respectively. 25,29 The substrate glucose is two-fold higher in He et al's report using recombinant cells, although better results were obtained. 41 Compared with these reports on wild-type strains for FAL conversion and FOL synthesis, the S. cerevisiae NL22 cells used in this study has much higher tolerance for FAL and FOL productivity.…”

“…Escherichia coli CCZU‐T15 cells were conducted at 1.6 mol glucose/mol FAL . Bacillus coagulans NL01 cells were used at 1.9 mol glucose/mol FAL . Obviously, the use of S. cerevisiae NL22 cells in this work seemed to need less glucose dosage, which may be relatively economical for the reduction process.…”

“…It is also observed that during the whole reacting process, the amount of FOL kept increasing and gradually reached a stable point along with the time increasing among all determined temperatures. According to past reports, B. coagulans NL01 could exhibit excellent FOL productivity (0.29 mmol h −1 ) at 50 ∘ C but 0.14 mmol h −1 at 45 ∘ C. 25 Meyerozyma guilliermondii SC1103 give its catalytic performances for furans, and the productivity of 2,5-bis(hydroxymethyl)furan (BHMF) and FOL are 0.03 mmol h −1 . 29 In addition, although some S. cerevisiae culturing cells were reported to be FAL-tolerant, their FOL productivity are very poor (0.015 mmol h −1 ).…”

“…23 Our group found that Bacillus coagulans GKN316 can effectively convert several aldehyde inhibitors to the corresponding alcohols, and B. coagulans NL01 could convert 100% FAL into FOL at 40 mmol L −1 . 24,25 The whole-cell bio-reduction of FAL remains challenging, since a higher tolerance strain for FAL needs to be looked for and a higher product concentration is required.…”