Efficient AuFeO_x Nanoclusters of Laser-Ablated Nanoparticles in Water for Cells Guiding and Surface-Enhanced Resonance Raman Scattering Imaging

Abstract

Nanoclusters with plasmonic and magnetic properties are obtained with naked gold (AuNP) and iron oxide nanoparticles (FeO x NP) separately synthesized by laser ablation in water and simply assembled by using their opposite surface charges. Controlling the amount of AuNP and FeO x NP, we obtained nanoclusters with both good surface-enhanced resonance Raman scattering (SERRS) signals and superparamagnetic properties. Nanoclusters are incubated with murine macrophage cells which, after mixing with other macrophages, can be magnetically guided in solution by sorting them in less than 10 min. SERRS signals, observed at the single cell level, were recorded for the sorted macrophages, showing that the nanoclusters are active also in a biological environment and allow the identification of the incubated macrophage cells.

Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes

“…, when particles with plasmonic and magnetic properties bind to cell membranes, 36 – 38 or when they enter a cell. 39 , 40 In bacteria, plasmonic–magnetic nanostructures were also shown to provide SERS spectral information from the molecules in the cell walls of bacterial cells. 41 – 43 …”

“…[30][31][32][33][34][35] Spectra of magnetic SERS labels were also employed to visualize their interaction with cells, e.g., when particles with plasmonic and magnetic properties bind to cell membranes, [36][37][38] or when they enter a cell. 39,40 In bacteria, plasmonic-magnetic nanostructures were also shown to provide SERS spectral information from the molecules in the cell walls of bacterial cells. [41][42][43] In the work presented here, we study the interaction of composite magnetic-plasmonic nanoprobes (Ag-Magnetite and Au-Magnetite) inside eukaryotic cells by observing the molecular composition in the proximity of the composite structures by SERS.…”

Solvent-surface interactions control the phase structure in laser-generated iron-gold core-shell nanoparticles

“…They showed how to combine the optical properties of gold, such as surface-enhanced Raman scattering (SERS), with magneto-responsive iron and iron oxide in ligand-free nanoparticles. An example of these materials’ potential for biomedical applications was given by the magnetic sorting of murine macrophages with enclosed AuFeO x -nanoparticles and the subsequent SERS-imaging of the sorted cells 36 . In another approach, the impact of the surrounding medium on the morphology of laser-generated FeAu nanoparticles was studied.…”

Hybrid Magneto-Plasmonic Nanoparticles in Biomedicine: Fundamentals, Synthesis and Applications

“…A less explored method is the electrostatic assembling of individual NP into an hybrid nanostructure [114]. The main limitations of the electrostatic assembly are the lack of stability in biological media and the low specificity in the assembly [115].…”