Efficient Assembly of Photosystem II in <i>Chlamydomonas reinhardtii</i> Requires Alb3.1p, a Homolog of Arabidopsis ALBINO3

Ossenbühl, Friedrich; Göhre, Vera; Meurer, Jörg; Krieger‐Liszkay, Anja; Rochaix, Jean‐David; Eichacker, Lutz A.

doi:10.1105/tpc.023226

Cited by 109 publications

(89 citation statements)

References 44 publications

(56 reference statements)

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“…To test whether they correspond to known photosynthetic complexes, the BN gel blots were probed with antibodies against a subunit of each complex. This revealed that the 300-and 600-kD complexes correspond to the reaction center core PSII monomer and dimer, respectively, because they react with D2 antibodies and are absent in FuD7 ( Figure 4A, aD2) (Ossenbuhl et al, 2004). A complex with slower migration than the PSII dimer is detectable at ;700 kD with PsaA antiserum, corresponding most likely to PSI-LHCI ( Figure 4A, aPsaA).…”

Section: Alb32 Is Associated With Psii Complexesmentioning

confidence: 97%

“…It is absent in F15 and strongly reduced in ac29. Instead, a new complex is detectable in ac29 at 300 kD corresponding to PSI without LHCI (Ossenbuhl et al, 2004). This is attributable to the considerable decrease of LHCs in ac29 (Bellafiore et al, 2002;Ossenbuhl et al, 2004).…”

Section: Alb32 Is Associated With Psii Complexesmentioning

confidence: 98%

“…Indeed, studies with C. reinhardtii have revealed a role for Alb3.1 in the assembly of the PSII reaction center polypeptide D1. PSII accumulation is reduced by 2-fold in light-grown cells and by nearly 10-fold in dark-grown cells, and integration of D1 into the core complex of PSII is slowed significantly in the Alb3.1-deficient mutant (Bellafiore et al, 2002;Ossenbuhl et al, 2004). Moreover, use of the yeast splitubiquitin system has revealed direct interactions between ALB3 and several thylakoid membrane proteins, such as D1, D2, CP43, PsaA, and the ATP synthase subunit CF 0 III (Pasch et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

et al. 2006

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Proteins of the YidC/Oxa1p/ALB3 family play an important role in inserting proteins into membranes of mitochondria, bacteria, and chloroplasts. In Chlamydomonas reinhardtii, one member of this family, Albino3.1 (Alb3.1), was previously shown to be mainly involved in the assembly of the light-harvesting complex. Here, we show that a second member, Alb3.2, is located in the thylakoid membrane, where it is associated with large molecular weight complexes. Coimmunoprecipitation experiments indicate that Alb3.2 interacts with Alb3.1 and the reaction center polypeptides of photosystem I and II as well as with VIPP1, which is involved in thylakoid formation. Moreover, depletion of Alb3.2 by RNA interference to 25 to 40% of wild-type levels leads to a reduction in photosystems I and II, indicating that the level of Alb3.2 is limiting for the assembly and/or maintenance of these complexes in the thylakoid membrane. Although the levels of several photosynthetic proteins are reduced under these conditions, other proteins are overproduced, such as VIPP1 and the chloroplast chaperone pair Hsp70/ Cdj2. These changes are accompanied by a large increase in vacuolar size and, after a prolonged period, by cell death. We conclude that Alb3.2 is required directly or indirectly, through its impact on thylakoid protein biogenesis, for cell survival.

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Section: Alb32 Is Associated With Psii Complexesmentioning

confidence: 97%

Section: Alb32 Is Associated With Psii Complexesmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

et al. 2006

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“…Two Alb3 proteins (Alb3.1 and Alb3.2) are present in Chlamydomonas, whereas only one Alb3 protein is present in Arabidopsis. Alb3.1 of Chlamydomonas is involved mainly in the insertion of the light-harvesting complex [22], and it is required for the insertion of D1 into functional PSII [23]. Alb3.2 interacts with Alb3.1 and the reaction centre polypeptides of PSI and PSII.…”

Section: Regulatory Factors For Psii Assemblymentioning

confidence: 99%

Regulatory factors for the assembly of thylakoid membrane protein complexes

Chi

Zhang

2012

Phil. Trans. R. Soc. B

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Major multi-protein photosynthetic complexes, located in thylakoid membranes, are responsible for the capture of light and its conversion into chemical energy in oxygenic photosynthetic organisms. Although the structures and functions of these photosynthetic complexes have been explored, the molecular mechanisms underlying their assembly remain elusive. In this review, we summarize current knowledge of the regulatory components involved in the assembly of thylakoid membrane protein complexes in photosynthetic organisms. Many of the known regulatory factors are conserved between prokaryotes and eukaryotes, whereas others appear to be newly evolved or to have expanded predominantly in eukaryotes. Their specific features and fundamental differences in cyanobacteria, green algae and land plants are discussed.

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“…Psb29 and the immonophilin FKBP20-2 are required for efficient assembly of PSII supercomplexes (Keren et al, 2005;Lima et al, 2006;Fu et al, 2007). LPA2 and LPA3 cooperate with each other and interact with ALB3 in assisting incorporation and stabilization of CP43 (Ossenbühl et al, 2004;Cai et al, 2010). Psb28 was suggested to be important for the synthesis of chlorophylls and/or apoproteins of the inner antenna subunit CP47 (Dobáková et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum

et al. 2014

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ORCID ID: 0000-0003-2973-9514 (J.M.)The chloroplast-encoded low molecular weight protein PsbN is annotated as a photosystem II (PSII) subunit. To elucidate the localization and function of PsbN, encoded on the opposite strand to the psbB gene cluster, we raised antibodies and inserted a resistance cassette into PsbN in both directions. Both homoplastomic tobacco (Nicotiana tabacum) mutants ΔpsbN-F and ΔpsbN-R show essentially the same PSII deficiencies. The mutants are extremely light sensitive and failed to recover from photoinhibition. Although synthesis of PSII proteins was not altered significantly, both mutants accumulated only ;25% of PSII proteins compared with the wild type. Assembly of PSII precomplexes occurred at normal rates, but heterodimeric PSII reaction centers (RCs) and higher order PSII assemblies were not formed efficiently in the mutants. The ΔpsbN-R mutant was complemented by allotopic expression of the PsbN gene fused to the sequence of a chloroplast transit peptide in the nuclear genome. PsbN represents a bitopic trans-membrane peptide localized in stroma lamellae with its highly conserved C terminus exposed to the stroma. Significant amounts of PsbN were already present in dark-grown seedling. Our data prove that PsbN is not a constituent subunit of PSII but is required for repair from photoinhibition and efficient assembly of the PSII RC.

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Efficient Assembly of Photosystem II in Chlamydomonas reinhardtii Requires Alb3.1p, a Homolog of Arabidopsis ALBINO3

Cited by 109 publications

References 44 publications

One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

One of Two Alb3 Proteins Is Essential for the Assembly of the Photosystems and for Cell Survival in Chlamydomonas

Regulatory factors for the assembly of thylakoid membrane protein complexes

PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum

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