Efficient Angiogenesis-Based Diabetic Wound Healing/Skin Reconstruction through Bioactive Antibacterial Adhesive Ultraviolet Shielding Nanodressing with Exosome Release

Wang, Min; Wang, Chenggui; Chen, Mi; Xi, Yang; Cheng, Wei; Mao, Cong; Xu, Tianzhen; Zhang, Xingxing; Lin, Cai; Gao, Weiyang; Guo, Yi; Lei, Bo

doi:10.1021/acsnano.9b03656

Cited by 369 publications

(299 citation statements)

References 77 publications

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“…Skin tissue contains two layers, the dermal layer and corium layer [9], and is composed of three major cell types: epithelial cells, fibrocytes and endothelial cells [10][11][12]. Fibrocytes, fibers, endothelial cells and capillaries construct the corium layer, whereas epithelial cells compose the dermal layer.…”

Section: Introductionmentioning

confidence: 99%

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

et al. 2020

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Background: Diabetic wounds are a disturbing and rapidly growing clinical problem. A novel peptide, parathyroid hormone related peptide (PTHrP-2), is assumed as multifunctional factor in angiogenesis, fibrogenesis and reepithelization. This study aims to test PTHrP-2 efficiency and mechanism in wound healing. Methods: Through repair phenomenon in vivo some problems were detected, and further research on their mechanisms was made. In vivo therapeutic effects of PTHrP-2 were determined by HE, Masson, microfil and immunohistochemical staining. In vitro direct effects of PTHrP-2 were determined by proliferation, migration, Vascular Endothelial Grown Factor and collagen I secretion of cells and Akt/ Erk1/2 pathway change. In vitro indirect effects of PTHrP-2 was study via exosomes. Exosomes from PTHrP-2 untreated and treated HUVECs and HFF-1 cells were insolated and identified. Exosomes were co-cultured with original cells, HUVECs or HFF-1 cells, and epithelial cells. Proliferation and migration and pathway change were observed. PTHrP-2-HUVEC-Exos were added into in vivo wound to testify its hub role in PTHrP-2 indirect effects in wound healing. Results: In vivo, PTHrP-2 exerted multifunctional pro-angiogenesis, pro-firbogenesis and re-epithelization effects. In vitro, PTHrP-2 promoted proliferation and migration of endothelial and fibroblast cells, but had no effect on epithelial cells. Therefore, we tested PTHrP-2 indirect effects via exosomes. PTHrP-2 intensified intercellular communication between endothelial cells and fibroblasts and initiated endothelial-epithelial intercellular communication. PTHrP-2-HUVEC-Exos played a hub role in PTHrP-2 indirect effects in wound healing.Conclusion: These findings of this study indicated that PTHrP-2, a multifunctional factor, could promote wound healing via synergistic multicellular stimulating and exosomal activities.

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Section: Introductionmentioning

confidence: 99%

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

et al. 2020

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“…Yet, such cellular vesicles exhibited a rapid clearance rate in vivo, with a relatively short half-life. Therefore, it would be convenient to increase their stability, once applied in vivo (Wang et al, 2019a). Different approaches have been carried out to reach such a goal, and increase the EV effectiveness.…”

Section: Angiogenesismentioning

confidence: 99%

“…Indeed, the FEP@exo stimulated the angiogenesis, formation of granulation tissue, collagen deposition, and re-epithelization of the skin. The efficiency of this scaffold dressing approach is thought to be due to several reasons: (i) maintaining a wet environment; (ii) absorbing wound secretions; (iii) acting as antimicrobial agent; (iv) protecting against the UV light; and (v) favoring angiogenesis, through the liberation of EV (Wang et al, 2019a).…”

Section: Angiogenesismentioning

confidence: 99%

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Casado-Díaz

Quesada‐Gómez

Dorado

2020

Front. Bioeng. Biotechnol.

204

183

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The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. The former are usually called exosomes, with sizes ranging from 50 to 100 nm. These EV contain a lipid bilayer associated to membrane proteins. Molecules such as nucleic acids (DNA, mRNA, miRNA, lncRNA, etc.) and proteins may be stored inside. The EV composition depends on the producer cell type and its physiological conditions. Through them, the cells modify their microenvironment and the behavior of neighboring cells. That is accomplished by transferring factors that modulate different metabolic and signaling pathways. Due to their properties, EV can be applied as a diagnostic and therapeutic tool in medicine. The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. These features are linked to their paracrine activity and EV secretion. Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. In this area, the use of EV for the treatment of chronic skin ulcers (CSU) has been proposed. Such sores occur when normal healing does not resolve properly. That is usually due to excessive prolongation of the inflammatory phase. These ulcers are associated with aging and diseases, such as diabetes, so their prevalence is increasing with the one of such latter disease, mainly in developed countries. This has very important socio-economic repercussions. In this review, we show that the application of MSC-derived EV for the treatment of CSU has positive effects, including accelerating healing and decreasing scar formation. This is because the EV have immunosuppressive and immunomodulatory properties. Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. They include endothelial cells, fibroblasts, and keratinocytes. Most of the studies carried out so far are preclinical. Therefore, there is a need to advance more in the knowledge about the conditions of production, isolation, and action mechanisms of EV. Interestingly, their potential application in the treatment of CSU opens the door for the design of new highly effective therapeutic strategies.

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“…Bioactive dressings are a good choice for the repair of diabetic wounds. As shown in Figure 1, researchers have prepared an injectable adhesive thermosensitive multifunctional polysaccharide-based dressing (fluorinated ethylenepropylene) that can continuously release exosomes to promote angiogenesis at the wound site and accelerate the healing process (Khanolkar et al, 2008;Wang et al, 2019). The silver ion foam dressing used in patients with diabetic foot maintains the wound moist.…”

Section: Dfusmentioning

confidence: 99%

Selection of Appropriate Wound Dressing for Various Wounds

Shi

Wang

et al. 2020

Front. Bioeng. Biotechnol.

243

157

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There are many factors involved in wound healing, and the healing process is not static. The therapeutic effect of modern wound dressings in the clinical management of wounds is documented. However, there are few reports regarding the reasonable selection of dressings for certain types of wounds in the clinic. In this article, we retrospect the history of wound dressing development and the classification of modern wound dressings. In addition, the pros and cons of mainstream modern wound dressings for the healing of different wounds, such as diabetic foot ulcers, pressure ulcers, burns and scalds, and chronic leg ulcers, as well as the physiological mechanisms involved in wound healing are summarized. This article provides a clinical guideline for selecting suitable wound dressings according to the types of wounds.

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Efficient Angiogenesis-Based Diabetic Wound Healing/Skin Reconstruction through Bioactive Antibacterial Adhesive Ultraviolet Shielding Nanodressing with Exosome Release

Cited by 369 publications

References 77 publications

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

Extracellular Vesicles Derived From Mesenchymal Stem Cells (MSC) in Regenerative Medicine: Applications in Skin Wound Healing

Selection of Appropriate Wound Dressing for Various Wounds

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