Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Surface wave dynamics in orbital shaken cylindrical containers

“…These reasons kindled the interest in the use of large scale orbital shaken devices up to thousand liters. [7][8][9] Thus, a large number of researches have been performed on the gas exchange, [10][11][12][13] mixing, [14][15][16][17] and volumetric power consumption 18 of the bioreactors. In some cases, correlations have been proposed in order to quantify the variation of those quantities during scale-up of the culture size.…”

High-density mammalian cell cultures in stirred-tank bioreactor without external pH control

“…To achieve such goals, additional realtime glucose/lactate monitoring equipment will have to be used, and this could add complexity in process development. Recently, a few studies have shown the possibility of maintaining good cell growth without pH and DO controls through optimizing engineering parameters of either Wave bioreactors or orbitally shaken bioreactors (OSR) for 7-14 days (Tissot et al, 2011;Yuk et al, 2011). However, none of them demonstrated desired pH control for longterm protein production.…”

The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4

“…For the ambr and 5L bioreactors, although the impeller speed and nitrogen flow rates were fixed, the dO 2 was controlled by varying the oxygen flow rate through the sparger, and hence the total flow rate and the driving force. It is possible that oxygen transfer rates in shake-flasks were so low that oxygen limitation occurred and it is known that imposing such an environmental stress on the cell can lead to lower cell numbers and higher protein yields [36]. Indeed, in the more extensive study of the biological aspects by Hsu et al [22], in 3 of the 4 cell lines studied, the protein yield was on average ~ 20% greater in the uncontrolled environment of the shake flask.…”