Efficient and Cost-Reduced Glucoamylase Fed-Batch Production with Alternative Carbon Sources

Abstract

Glucoamylase is an important industrial enzyme. Glucoamylase production by industrial Aspergillus niger strain featured with two major problems: (i) empirical substrate feeding methods deteriorating the fermentation performance; and (ii) the high raw materials cost limiting the economics of the glucoamylase product with delegated specification. In this study, we first proposed a novel three-stage varied-rate substrate feeding strategy for efficient glucoamylase production in a 5 L bioreactor using the standard feeding medium, by comparing the changing patterns of the important physiological parameters such as DO, OUR, RQ, etc., when using different substrate feeding strategies. With this strategy, the glucoamylase activity and productivity reached higher levels of 11,000 U/ml and 84.6 U/ml/h, respectively. The performance enhancement in this case was beneficial from the following results: DO and OUR could be controlled at the higher levels (30%, 43.83 mmol/l/h), while RQ was maintained at a stable/lower level of 0.60 simultaneously throughout the fed-batch phase. Based on this three-stage varied-rate substrate feeding strategy, we further evaluated the economics of using alternative carbon sources, attempting to reduce the raw materials cost. The results revealed that cornstarch hydrolysate could be considered as the best carbon source to replace the standard and expensive feeding medium. In this case, the production cost of the glucoamylase with delegated specification (5,000 U/ml) could be saved by more than 61% while the product quality be ensured simultaneously. The proposed strategy showed application potential in improving the economics of industrial glucoamylase production.

Feeding strategy impacts heterologous protein production in Yarrowia lipolytica fed‐batch cultures—Insight into the role of osmolarity

“…Fed‐batch cultivations conducted at high‐cell densities are known, frequently adopted strategy for maximization of volumetric productivity of biomolecules, which synthesis is associated with biomass growth (Kim et al, ; Tapia et al, ). Recently, an interesting example of optimization strategy applied to a fed‐batch process oriented on production of native glucoamylase by industrial Aspergillus niger strain, has been published by Luo et al (Luo, Liu, He, Zhou, & Shi, ). In that study, after initial stage of culturing according to a batch approach, three different feeding modes were tested: (a) maintaining DO‐stat (constant concentration of dissolved oxygen), (b) continuous feeding with constant substrate provision, (c) continuous feeding with variable substrate provision, driven by several respiratory parameters.…”

Analysis of the Biotechnological Potential of a Lentinus crinitus Isolate in the Light of Its Secretome

“…The rationale behind this is the previously observed 42 synergistic effect among C and N sources and the use of low-cost carbon sources (glycerol and starch) that can be obtained as byproducts of the synthesis of biodiesel and agroindustry. 43,44 Notably, changes in nitrogen content resulted in distinct protein secretion profiles according to the carbon source (Figures 3 and 4). In glycerol-and maltose based-media, different N sources and changes in N concentration had little effect on fungal growth.…”

“…Maltose-based medium was chosen because of the complex pattern of secreted proteins, while glycerol- and starch-based media resulted in low protein yields. The rationale behind this is the previously observed synergistic effect among C and N sources and the use of low-cost carbon sources (glycerol and starch) that can be obtained as byproducts of the synthesis of biodiesel and agroindustry. , …”

Optimisasi pH dan Agitasi pada Produksi Glukoamilase dari Saccharomycopsis fibuligera R64 Menggunakan Response Surface Method

“…Perbandingan dedak beras dengan ekstrak ragi sebesar 2:1 merupakan perbandingan komposisi media optimum untuk produksi enzim α-amilase dari S. fibuligera (Safari et al 1996) dan media produksi enzim αamilase dari S. fibuligera dengan sumber karbon tepung beras menunjukkan aktivitas tertinggi kedua setelah media produksi dengan komposisi sagu (Ishmayana et al 2008). Luo et al (2015) telah memproduksi enzim glukoamilase dari Aspergillus niger dengan berbagai sumber karbon seperti maltosa, glukosa, hidrolisat singkong dan hidrolisat pati jagung dengan menggunakan alat fermentor dengan skala 5 L. Hasil penelitiannya menunjukkan produksi enzim glukoamilase tertinggi dihasilkan pada sumber karbon hidrolisat pati jagung dengan nilai aktivitas sebesar 14.500 unit/mL.…”

“…Penelitian sebelumnya memberikan informasi bahwa enzim glukoamilase telah banyak di produksi dengan berbagai variabel penelitian yang diujikan (Luo et al 2015;Wang et al 2008). Namun variabel penelitian yang difunakan masih bersifat tunggal dan tidak diketahui pengaruh interaksi antar variabel pada produksi glukoamilase.…”