Efficient and cost-effective production of Bacillus thuringiensis subsp. aizawai spores and delta-endotoxins using agricultural raw materials and agro-industrial wastes under submerged fermentation

Gounina-Allouane, Rabia; Acheuk, Fatma; Fatma, Sahir-Halouane

doi:10.1016/j.biteb.2022.101001

Cited by 4 publications

(4 citation statements)

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“…A recent study used a cost-effective liquid culture media based on agricultural raw materials (edible leguminous seeds, corn, wheat bran, dry palm, date pulps, and corn forages) and agroindustrial wastes (dry dates palm pits and olive mill solid wastes) for spores and delta-endotoxins production by a B. thuringiensis subsp. Aizawai strain [24]. The dry date palm extract was the most efficient medium for spore production and presented 1.43 × 10 10 spores ml −1 after 72 h of incubation.…”

Section: Discussionmentioning

confidence: 93%

“…The presence of these organic compounds promotes bacterial growth and activates genes related to toxin production, leading to a higher production efficiency of these compounds, using materials that would be discarded [7,35]. A wide variety of commercial culture media and industrial substrates have been tested for optimizing Bt production [15,18,24,61] using wastes from starch industry, sewage plants, the poultry industry, slaughterhouses, agro-business and restaurants [15]. A recent study used a cost-effective liquid culture media based on agricultural raw materials (edible leguminous seeds, corn, wheat bran, dry palm, date pulps, and corn forages) and agroindustrial wastes (dry dates palm pits and olive mill solid wastes) for spores and delta-endotoxins production by a B. thuringiensis subsp.…”

Section: Discussionmentioning

confidence: 99%

“…The dry date palm extract was the most efficient medium for spore production and presented 1.43 × 10 10 spores ml −1 after 72 h of incubation. The chickpea medium showed a production of 581 μg ml −1 delta-endotoxins, displaying the most efficient results [24]. Starch industry wastewater was also investigated for Bt kurstaki fermentation, presenting a high total cell count of 2.90 × 10 8 CFU ml −1 , viable spores of 2.4 × 10 8 CFU ml −1 and a maximum endotoxin concentration of 435 µg ml −1 after 48 h fermentation [36].…”

Section: Discussionmentioning

confidence: 99%

“…The use of industrial substrates used in its production generally increase the final price of Bt-based products. The possible replacement of synthetic fermentation media by equally effective natural raw materials, especially the use of agro-industrial waste as substrates is a promising as well as an environmentally sustainable option with positive economic impacts [15,24].…”

Section: Introductionmentioning

confidence: 99%

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Use of agro-industrial bio-waste for the growth and production of a previously isolated Bacillus thuringiensis strain

dos Santos,

de S. Varize,

Valença

et al. 2024

Beni-Suef Univ J Basic Appl Sci

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Background Bacillus thuringiensis (Bt) is a widely used biopesticide. The bioinsecticide based on Bt is obtained by fermentation, but the substrates currently used for its production constitute ingredients of high commercial value. In this context, the use of agro-industrial residues as substrates is an alternative to make the fermentation process viable on a large scale, in addition to minimizing environmental problems and contributing to the destination of these residues for biotechnological purposes. Results In the first part of this study, a previously isolated spore forming soil bacteria (Bv5) harboring and expressing a novel cry 8A gene was confirmed as B. thuringiensis based on its morphological characteristics, Gram staining, scanning electron microscopy (SEM) and genome sequencing. Bv5 was established as a Gram-positive spore forming bacteria with ellipsoidal spores and small round toxins. Bv5 genome comprised of the 5.30 Mb chromosome and two megaplasmids of 450 kb and 261 kb, respectively, with cry 8A gene located on the smallest megaplasmid. In the second part of the study, the physiological profile of the Bv5 strain during fermentation in different agro-industrial biowastes (cassava wastewater, orange pulp wash and whey) was analyzed. The fermentation experiment was divided into two stages. In the first stage, the agro-industrial waste with or without salts with the best results for biomass, spores and proteins production was selected. In the second stage, the effect of the selected medium in original and diluted form with the C:N balance was evaluated, in two different fermentation times (72 h and 96 h). Pulp wash enriched with salts was selected as the most suitable medium for the growth of Bv5 strain in the first stage. In the second stage pulp wash (without dilution) with the addition of salts, and with nitrogen supplementation, was considered the best for cell growth, spore and toxin production by Bv5. Conclusions To conclude, our study provide a new alternative for bio-waste from the orange juice industry, as well as potential culture medium for the Bt commercial scale production.

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Section: Discussionmentioning

confidence: 93%