Efficient Agrobacterium tumefaciens-mediated transformation and regeneration of garlic (Allium sativum) immature leaf tissue

Abstract

Transgenic garlic (Allium sativum) plants have been recovered directly from immature leaf material by selective culture following Agrobacterium-mediated transformation. This method involved the use of a binary vector containing the mgfp-ER reporter gene and hpt selectable marker, and followed a similar protocol developed previously for the transformation of immature onion embryos. The choice of tissue and post-transformation selection procedure resulted in a large increase in recovery of transgenic plants compared with previously confirmed allium transformation protocols. The presence of transgenes in the genome of the plants was confirmed using Southern analysis. This improvement in frequency and the use of clonal commercial "Printanor" germplasm now makes possible the integration of useful agronomic and quality traits into this crop.

Development of an Efficient Agrobacterium-Mediated Transformation System and Production of Herbicide-Resistant Transgenic Plants in Garlic (Allium sativum L.)

“…The transformation frequencies for garlic Danyang ranged from 1.1% to 10.6%, which was calculated by dividing the number of hygromycin-resistant and sGFP expressing plantlets by the total number of co-cultivated calli (Table 2). This transformation efficiency via Agrobacterium mediation is higher than previously reported (Kenel et al, 2010).…”

“…More recently, calli derived from different explant sources including roots as the most easily available explant and an Agrobacterium-mediated procedure were used to produce agronomically useful transgenic garlic plants resistant to beet armyworm (Spodoptera exigua), a polyphagous insect (Zheng et al, 2004). In addition, the Agrobacteriummediated transformation of garlic was also reported using embryos (Eady et al, 2005) and immature leaf materials (Kenel et al, 2010). These recent garlic transformation methods are still inefficient, although more transgenic events were obtained.…”

Enhanced Virulence Gene Activity of Agrobacterium in Muskmelon (Cucumis melo L.) cv. ‘Birdie’

“…Our observation confirmed that LBA4404 strain is most suitable for muskmelon transformation study. This strain found suitable for successful transformation study in many other crops such as potato with 58.4% of internode explants (Sarker et al, 2009) and garlic (Kenel et al, 2010). Different rates of crown gall induction obtained from the same explant, inoculated with different A. tumefaciens strains evidently suggest that the rates of tumorigenicity are strain-dependent.…”

“…lotus ( Jian et al, 2009), Brassica sp. (Kong et al, 2009) and garlic (Kenel et al, 2010). The present study was carried out to enhance the vir gene activity of different wild type Agrobacterium species through the application of chemical compounds along with physical factors and evaluate the vir gene activity through the phenotypic expression i.e.…”

Morfogênese de plantas de alho in vitro: papel dos reguladores de crescimento na indução e desenvolvimento de bulbos

“…Diversos ensaios foram realizados in vitro com o gênero Allium focando vários objetivos, tais como: conservação de germoplasma (ELLIS et al, 2005), estudos sobre embriogênese somática (FEREOL et al, 2002) e transformação genética (KENEL et al, 2010). Entretanto, a limpeza clonal através da cultura de ápices caulinares é, sem dúvida, a técnica de cultura de tecidos vegetais mais regularmente aplicada às espécies desse gênero.…”