Efficient adventitious shoot regeneration from shoot tip culture of Vanda coerulea, a Thai orchid

Abstract

Vanda coerulea is a popular albeit endangered blue orchid of Thailand, which would be desirable to propagate using regeneration methods. Here, we studied the effects of culture media (Vacin and Went, and Murashige and Skoog), sucrose concentrations (0-30 g/l), and plant growth regulators (BA, TDZ, and NAA) on adventitious shoot regeneration from shoot tip culture of V. coerulea. Shoot tips cultured on modified Vacin and Went (VW) medium supplemented with 10 g/l sucrose showed higher shoot and root formations, as well as plantlet height than those cultured on Murashige and Skoog medium. Addition of 1 mg/l BA to the modified VW medium induced the best shoots and roots after 3 months of culture. The combination of 0.5 mg/l NAA and 2 mg/l TDZ gave the optimal number of roots per explant and plantlet height after 3 months of culture. Survival rate of plantlets cultured in the greenhouse was 100% after 3 months of culture. There were no differences in morphology or patterns of ploidy level between stock plants and regenerated plants.

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

“…PGR(s) proportions have been known to have species-specific effect on callus and PLB induction from leaf explants (Shimura & Koda, 2004;Gantait, Bustam, & Sinniah, 2012). TDZ is a substituted phenyl urea with cytokinin-like activity (van Staden, Zažímalová, & George, 2008;Jitsopakul, Thammasiri, & Ishikawa, 2013). The ascendancy of TDZ in inducing somatic embryos (infant PLBs) from leaf explants was recounted in numerous orchid species including Oncidium (Mayer, Stancato, & Appezzato-Da-Glória, 2010), Doritaenopsis (Park, Yeung, Chakrabarty, & Paek, 2002), Dendrobium and Phalaenopsis (Kuo, Chen, & Chang, 2005).…”

“…The ascendancy of TDZ in inducing somatic embryos (infant PLBs) from leaf explants was recounted in numerous orchid species including Oncidium (Mayer, Stancato, & Appezzato-Da-Glória, 2010), Doritaenopsis (Park, Yeung, Chakrabarty, & Paek, 2002), Dendrobium and Phalaenopsis (Kuo, Chen, & Chang, 2005). TDZ is also useful for rapid plant regeneration through organogenesis (Kishor & Devi, 2009;Jitsopakul, Thammasiri, & Ishikawa, 2013), somatic em-bryogenesis (Jheng, Do, Liauh, Chung, & Huang, 2006;Gow, Chen, & Chang, 2009), or PLB formation of orchids (Mayer, Stancato, & Appezzato-Da-Glória, 2010;Khoddamzadeh et al, 2011;Roy, Sajeev, Pattanayak, & Deka, 2012). NAA is a synthetic auxin plant growth regulator that is routinely used in in vitro propagation of orchid to promote callus induction such as in Dendrobium (Mei, Danial, Mahmood, & Subramaniam, 2012;Salam, Salam, & Mohanty, 2013), Vanilla (Tan, Chin, & Alderson, 2011), Oncidium (Mayer, Stancato, & AppezzatoDa-Glória, 2010), Phalaenopsis (Khoddamzadeh et al, 2011), and Renanthera (Wu et al, 2012).…”

Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

“…The negative effect of NAA used in a concentration of 0.5 mg·dm −3 on the number of leaves was noted by Kishor and Sharma [ 28 ], in their experiment on the micropropagation of Renanthera imschootiana and Vanda coerulea . Different results were obtained by Talukder et al [ 29 ] in Dendrobium , as well as by Jitsopakul et al [ 30 ] in Vanda coerulea . The authors showed that the use of NAA in a concentration of 0.5 mg·dm −3 had a positive effect on the number of leaves of the tested species.…”

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

“…Moreover TDZ (0.5 mg/l) with NAA (1.0 mg/l) could provide a high number of microshoots/seedlings of hybrid orchid (Aerides vandarum Reichb.f X Vanda strangeana Reichb.f) (Kishor and Devi 2009). Jitsopakul et al (2013) also reported that MVM medium containing TDZ (2.0 mg/l) with NAA (0.5 mg/l) provide a high mean number of shoots/explants of Vanda coerulea. The combination of TDZ (1.0 mg/l) and NAA (0.5 mg/l) was also stimulated the formation of callus and proliferation from node, internode and leaf explants of Anoectochilus elatus on Mitra medium (Ahamed Sherif et al 2016).…”