Efficient Adeno-associated Virus–mediated Transgenesis in Alveolar Stem Cells and Associated Niches

Konkimalla, Arvind; Elmore, Zachary C.; Konishi, Satoshi; Macadlo, Lauren; Katsura, Hiroaki; Tata, Aleksandra; Asokan, Aravind; Tata, Purushothama Rao

doi:10.1165/rcmb.2022-0424ma

Cited by 5 publications

(1 citation statement)

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“…In the case of AdSCs, delivery of transgenes via electroporation or viruses also has challenges, as, in many cases, these techniques have not been well established or are not efficient. AdSCs may also activate stress pathways and lose their ability to self-renew ( 90 , 91 ). Therefore, more efforts are needed to optimize and benchmark methods for delivery of multiple DNA constructs of varying sizes, achieve efficient gene editing, and determine robust cellular markers to assess cell identities after gene manipulation.…”

Section: Innovative Biological Insights Provided By Organoid Gene Edi...mentioning

confidence: 99%

Recent advances in lung organoid development and applications in disease modeling

Vazquez-Armendariz,

Tata

2023

Journal of Clinical Investigation

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Over the last decade, several organoid models have evolved to acquire increasing cellular, structural, and functional complexity. Advanced lung organoid platforms derived from various sources, including adult, fetal, and induced pluripotent stem cells, have now been generated, which more closely mimic the cellular architecture found within the airways and alveoli. In this regard, the establishment of novel protocols with optimized stem cell isolation and culture conditions has given rise to an array of models able to study key cellular and molecular players involved in lung injury and repair. In addition, introduction of other nonepithelial cellular components, such as immune, mesenchymal, and endothelial cells, and employment of novel precision gene editing tools have further broadened the range of applications for these systems by providing a microenvironment and/or phenotype closer to the desired in vivo scenario. Thus, these developments in organoid technology have enhanced our ability to model various aspects of lung biology, including pathogenesis of diseases such as chronic obstructive pulmonary disease, pulmonary fibrosis, cystic fibrosis, and infectious disease and host-microbe interactions, in ways that are often difficult to undertake using only in vivo models. In this Review, we summarize the latest developments in lung organoid technology and their applicability for disease modeling and outline their strengths, drawbacks, and potential avenues for future development.

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Section: Innovative Biological Insights Provided By Organoid Gene Edi...mentioning

confidence: 99%