Efficient accumulation of high-value bioactive substances by carbon to nitrogen ratio regulation in marine microalgae Porphyridium purpureum

Li, Shaohua; Ji, Liang; Chen, Cheng; Shu-xin, Zhao; Sun, Meng; Gao, Zhengquan; Wu, Haizhen; Fan, Jianhua

doi:10.1016/j.biortech.2020.123362

Cited by 66 publications

(37 citation statements)

References 44 publications

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“…Lachmann et al (2019) reported that NO3 grown Chlamydomonas acidophila accumulated more amino acids and protein compared to culture provided with NH +4 . Choi and Lee (2015) and Li, Ji, et al (2020) conducted that nitrogen and carbon compounds per algal cell increased with an increasing N/P ratio of the cultivated media. The chemical composition and fatty acids profile produced by microalgae can be controlled by environmental conditions and nutrient contents (Elshobary et al, 2019; Huerlimann et al, 2010; Li, Ji, et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Choi and Lee (2015) and Li, Ji, et al (2020) conducted that nitrogen and carbon compounds per algal cell increased with an increasing N/P ratio of the cultivated media. The chemical composition and fatty acids profile produced by microalgae can be controlled by environmental conditions and nutrient contents (Elshobary et al, 2019; Huerlimann et al, 2010; Li, Ji, et al, 2020). In the current study, the water source was significantly affected, biomass, biomass productivity and biochemical composition especially fatty acids, and this could be due to the sensitivity and adaptability of each species to the physico‐chemical properties of water.…”

Section: Discussionmentioning

confidence: 99%

“…These fatty acids and other algal components vary depending on the cultured species, light source, temperature and growth stage (Subhash et al, 2014). Available microalgae, such as Nannochloropsis , is rich in n ‐3 oils, which can be commercially used for Rotifer and Artemia enrichment as well as improved feed ingestion and growth (Ashour et al, 2019; Li, Ji, et al, 2020; Sørensen et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Comparative study on growth, survival and pigmentation ofSolea aegyptiacalarvae by using four different microalgal species with emphasize on water quality and nutritional value

et al. 2020

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This study aimed to evaluate appropriate water sources to improve Solea aegyptiaca aquaculture from larval to the juvenile stage using two water sources (Eastern Harbour (EH) and MaxWell (MW)). Firstly, four microalgae's nutritional value (Nannochloropsis salina, N. oculata, Chlorella salina and Tetraselmis chuii) was assessed in both water sources. MW of high nitrate content enhanced the algal biomass and biochemical composition of all species compared to EH. MW-N. salina showed the highest growth and biochemical contents among the investigated species and yielded Artemia franciscana with a considerable amount of arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) compared to EH. Secondly, Artemia enriched MW-N salina was used to improve S. aegyptiaca quality in both water sources from 10-48 days posthatch at a density of 15 larvae L −1. The MW-S. aegyptiaca exhibited a significant increase in morphometric parameters, albino percentages and recorded the highest ARA, EPA and DHA content (35.9, 6.1 and 15.9 µg g −1 , respectively) compared to that reared on EH. The study reports evidence of albinism of MW-S. aegyptiaca due to high dietary and ARA content. Overall, the seawater source has a significant impact on the whole food chain quality from microalgae to S. aegyptiaca.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative study on growth, survival and pigmentation ofSolea aegyptiacalarvae by using four different microalgal species with emphasize on water quality and nutritional value

et al. 2020

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“…Apart from nitrogen, sulphur and phosphorus are also essential (Markou and Nerantzis 2013). In one of the recent reports which was done with culture of Porphyridium purpureum, it was shown that there was accumulation of EPS with the increase in carbon/nitrogen ratio (Li et al 2020). Enhanced EPS production was reported in nitrogen and sulphate deficiency in Rhodella reticulate (Arad et al 1992;Sutherland 1996).…”

Section: Discussionmentioning

confidence: 99%

Scenedesmus acutus extracellular polysaccharides produced under increased concentration of sulphur and phosphorus exhibited enhanced proliferation of peripheral blood mononuclear cells

Patwal

Baranwal

2021

3 Biotech

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Exopolysaccharides (EPS) isolated from microalgae are promising immune cell proliferation agents, that could be potentially used as immunostimulants. In the current study, Scenedesmus acutus ( S. acutus ) was grown under varying nutrient (sulphur and phosphorus) concentrations to enhance the EPS production, and the isolated EPS were assessed for their effect on cell proliferation using peripheral blood mononuclear cells (PBMC). Five different concentrations of MgSO 4 (0, 0.25, 0.5, 1.0 and 1.25 g/L) and K 2 HPO 4 (0, 0.2, 0.6, 0.8 and 1.0 g/L) were taken as compared to the normal culture conditions (0.75 g/L MgSO 4 and 0.4 g/L K 2 HPO 4 ) with the intention to enrich EPS secretion. LC–MS, FTIR and NMR analysis revealed that isolated EPS have the characteristic spectrum of hetero-polysaccharides (octa-saccharides). Immunostimulatory property of EPS was demonstrated by their ability to augment PBMC proliferation as measured by MTT assay. Further, increase in the glucose content and proliferative index was observed for EPS obtained under higher concentrations of MgSO 4 (1 and 1.25 g/L) and K 2 HPO 4 (0.6 and 0.8 g/L) relative to normal culture conditions. Effects of the generated EPS under varying concentration of MgSO 4 ( r = 0.84–0.99) and K 2 HPO 4 ( r = 0.76–0.97) remained strongly correlated with cell count, chlorophyll content, total biomass, glucose, proliferative index and its scavenging activity. Collectively, our data not only showed that EPS generated by S. acutus under higher concentration of K 2 HPO 4 and MgSO 4 possess improved immunostimulatory properties, but also provides convincing evidence towards nutritional optimization of alga for enhanced EPS production with better bioactivities. Supplementary Information The online version contains supplementary material available at 10.1007/s13205-021-02720-z.

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“…This microalga performs photosynthesis by the lamella-shaped thylakoids that host the photosynthetic material such as phycobiliproteins (phycoerythrin, phycocyanin, and allophycocyanin) and chlorophyll. This latter is located inside the thylakoids, and the phycobiliproteins are organized in a hemispherical structure called phycobilisome located outside of the thylakoid membrane [ 1 , 2 ]. P. purpureum is an invaluable source of several natural active compounds.…”

Section: Introductionmentioning

confidence: 99%

Implementation of kLa-Based Strategy for Scaling Up Porphyridium purpureum (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins

et al. 2021

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Porphyridium purpureum is a well-known Rhodophyta that recently has attracted enormous attention because of its capacity to produce many high-value metabolites such as the pigment phycoerythrin and several high-value fatty acids. Phycoerythrin is a fluorescent red protein-pigment commercially relevant with antioxidant, antimicrobial activity, and fluorescent properties. The volumetric mass transfer coefficient (kLa) was kept constant within the different scaling-up stages in the present study. This scaling-up strategy was sought to maintain phycoerythrin production and other high-value metabolites by Porphyridium purpureum, using hanging-bag photobioreactors. The kLa was monitored to ensure the appropriate mixing and CO2 diffusion in the entire culture during the scaling process (16, 80, and 400 L). Then, biomass concentration, proteins, fatty acids, carbohydrates, and phycoerythrin were determined in each step of the scaling-up process. The kLa at 16 L reached a level of 0.0052 s−1, while at 80 L, a value of 0.0024 s−1 was achieved. This work result indicated that at 400 L, 1.22 g L−1 of biomass was obtained, and total carbohydrates (117.24 mg L−1), proteins (240.63 mg L−1), and lipids (17.75% DW) were accumulated. Regarding fatty acids production, 46.03% palmitic, 8.03% linoleic, 22.67% arachidonic, and 2.55% eicosapentaenoic acid were identified, principally. The phycoerythrin production was 20.88 mg L−1 with a purity of 2.75, making it viable for food-related applications. The results of these experiments provide insight into the high-scale production of phycoerythrin via the cultivation of P. purpureum in an inexpensive and straightforward culture system.

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Efficient accumulation of high-value bioactive substances by carbon to nitrogen ratio regulation in marine microalgae Porphyridium purpureum

Cited by 66 publications

References 44 publications

Comparative study on growth, survival and pigmentation ofSolea aegyptiacalarvae by using four different microalgal species with emphasize on water quality and nutritional value

Comparative study on growth, survival and pigmentation ofSolea aegyptiacalarvae by using four different microalgal species with emphasize on water quality and nutritional value

Scenedesmus acutus extracellular polysaccharides produced under increased concentration of sulphur and phosphorus exhibited enhanced proliferation of peripheral blood mononuclear cells

Implementation of kLa-Based Strategy for Scaling Up Porphyridium purpureum (Red Marine Microalga) to Produce High-Value Phycoerythrin, Fatty Acids, and Proteins

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