Efficiency of transrectal ultrasonography for pregnancy diagnosis of Ile de France ewes during the first month of gestation

Abstract

The aim of the present study was to test transrectal real-time B-mode ultrasonography for pregnancy diagnosis in ewes from the Ile de France breed on day 16, 23 and 30 after fixed-time artificial insemination. The experiment was carried out with 22 multiparous pure-bred Ile de France ewes aged 2-5 years. Diagnosis of pregnancy was based on the presence of enlarged uterine lumen with amniotic fluid and visualisation of embryo. The accuracy (Ac %), sensitivity (Se %) and specificity (Sp %) of the technique were determined. The gestational phase influenced significantly (P<0.01) the accuracy of the transrectal ultrasonography approach for pregnancy diagnosis. At day 16 the accuracy was 50.0%, whereas at day 23 and at day 30 it reached 72.73% and 90.91% respectively.

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“…The Acc of the STAT1 expression method was better than that of ultrasound in the earlier stage of pregnancy on days 15 and 25. Additionally, the Acc is low before day 23 (50%), but increases by days 23-30 (73 -91%) [39], it is lower than our finding on day 25.…”

“…On days 35 and 50 of pregnancy, low Se, Sp, and Acc were monitored compared to other methods (ultrasonography, progesterone, and PAG) [35,39,42,43], therefore, it is less important than earlier pregnancy periods (15 and 25 PI). These results were approved by a pairwise comparison test of AUC in the ROC curves, it turns out that days 15 and 25 were highly significant in pregnancy detection compared to each days 35 and 50.…”