Efficiency of the RAPD Molecular Indicator to Determine the Genetic Polymorphism of Flax Genotypes

Abstract

An experiment was carried out during the winter season 2022-2023, using ten genotypes of flax (2 Sakiha, 5 Sakiha, 7 Sakiha, 8 Sakiha, 9 Sakiha, Giza 3, Giza 4, Giza 5, Pure line 107, and Blanka). To determine the genetic fingerprint of flax genotypes. RAPD markets were used to evaluate genetic polymorphism ce between 10 genotypes of flax. All 11 primers produced 142 bands, at a rate of 11.83 bands per primer. The number of polymorphism t pieces ranged between 15 for primer UBC556 and 9 for primer A82, with a molecular weight that ranged between 400-2250 bp. The total number and percentage reached For dissimilar pieces, 125 and 88.08%, respectively. The highest percentage of discrepancies reached 100% for primers UBC604 and B24. Based on the binary data, genetic similarity, and the relatedness chart, the flax genotypes were separated into major and secondary groups. Analysis of the groups under the comparison ratio of the combinations within the relatedness chart showed that the variety Sakiha 5 with the structure Giza 4 and the structure Pure line 107 gave the highest genetic dimension, reaching 0.90 and 0.88, respectively. Perhaps this will be reflected in the flax later. The results indicated that RAPD parameters are highly efficient in diagnosing purity and genetic polymorphism between flax genotypes, so efficient selection of subsequent generations is possible.