Efficiency of Piglet Production after Transfer of the Vitrified Embryos at the Blastocyst and the Expanded Blastocyst Stages Collected Individually from Breeding Sows

Omagari, Hideaki; Misumi, Koji; Miyashita, Miho; Nagafuchi, Shigeki; Misawa, Hiroyasu; Yamashita, Shoko; Hoshi, Hiroyuki; Hirayama, Yuri; Yoshioka, Koji

doi:10.5938/youton.52.1

Cited by 1 publication

(5 citation statements)

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“…The survival rate to term of V/W ExB after Ns‐ET without AI was 25.2%, regardless of the semen origin (Table ). These results were equivalent to the reproductive performance of S‐ET using MVAC method reported previously (Misumi et al., 2013; Omagari et al., 2015; Sakagami et al., 2016). Consequently, it is considered that AI prior to Ns‐ET might overcome the disadvantage of Ns‐ET.…”

Section: Discussionsupporting

confidence: 86%

“…In previous studies (Cuello et al., 2005; Gomis et al., 2012; Martinez et al., 2015) of Ns‐ET for V/W blastocysts, asynchronous recipients whose estrous cycles were delayed by 1 day relative to the donors produced living piglets. In addition, piglet production from S‐ET of V/W blastocysts by the MVAC method was demonstrated for recipients whose estrous cycles were asynchronous with those of the donors by a 1‐ or 2‐day delay (Misumi et al., 2013; Omagari et al., 2015; Sakagami et al., 2016). Our results are similar to those reports; therefore, recipients whose estrous cycle are asynchronous with a 1‐ or 2‐day delay are adequate for non‐surgical deep intrauterine transfer of V/W ExBs using the MVAC method.…”

Section: Discussionmentioning

confidence: 99%

“…When the average number of surgically transferred V/W ExBs using the MVAC method is less than 15, the farrowing rate and survival rate to term are more than 70% and 20% respectively (Omagari et al., 2015). However, in the present study, the farrowing rate and survival rate to term after Ns‐ET were markedly inferior to those by S‐ET.…”

Section: Discussionmentioning

confidence: 99%

“…Omagari et al. (2015) and Sakagami et al. (2016) have also achieved acceptable reproductive performance using this approach.…”

Section: Introductionmentioning

confidence: 93%

“…In this context, Misumi et al (2013) have succeeded in producing a living piglet after S-ET of 8-15 V/W expanded blastocysts (ExBs) using the micro volume air cooling (MVAC) method. Omagari et al (2015) and Sakagami et al (2016) have also achieved acceptable reproductive performance using this approach. In addition, it is worth noting that the MVAC method employs chemically defined media for vitrification and warming.…”

mentioning

confidence: 94%

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Insemination of recipient sows improves the survival to term of vitrified and warmed porcine expanded blastocysts transferred non‐surgically

Tajima¹,

Uchikura²,

Kurita³

et al. 2020

Animal Science Journal

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This study was performed to evaluate reproductive performance after non‐surgical embryo transfer (Ns‐ET) of 10–15 porcine expanded blastocysts (ExBs) that had been vitrified and warmed (V/W) using the micro volume air cooling (MVAC) method. The effect of asynchrony between the donor and recipient estrous cycle was investigated. Ns‐ET was conducted in recipients whose estrous cycle was asynchronous to that of donors by a delay of 2, 1, or 0 days. In the 2‐day and 1‐day groups, the similar farrowing rates (27.3% and 25.0%) and survival rates to term (13.9% and 15.7%) were obtained after Ns‐ET of V/W ExBs. None of the recipients in 0‐day group farrowed. Artificial insemination (AI) prior to Ns‐ET was then evaluated. Ten–15 V/W ExBs were transferred non‐surgically to 12 recipients whose estrous cycles were asynchronous to that of donors by a 2‐day delay. All of the recipients produced piglets, and all (100.0%) delivered piglets were derived from the transferred V/W ExBs. The survival rate of V/W ExBs to term was 25.2%. These results demonstrate that Ns‐ET of V/W ExBs using MVAC can facilitate piglet production, even if 10–15 embryos are transferred. Moreover, piglets were obtained stably when AI was performed prior to Ns‐ET.

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Section: Discussionsupporting

confidence: 86%